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Citations for Miltenyi Biotec :
201 - 250 of 492 citations for Transmembrane protein 88 TMEM88 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cell Biology 2024Quote: ... The MicroBeads/cell extracts mixture was then applied to the μColumn followed by gentle wash three times with the lysis buffer used above for protein extraction (Miltenyi Biotec). Pre-heated (95°C ...
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bioRxiv - Cell Biology 2024Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer and incubated with the CD31-APC antibody (Miltenyi Biotec) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated for 15 min with CD11b antibody conjugated magnetic beads (Miltenyi Biotec) before passage through a magnetic bead column (Quadro MACS Separator and LS columns ...
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bioRxiv - Cancer Biology 2022Quote: ... The antibodies chosen for the staining were: anti-GFAP APC (130-124-040, Miltenyi Biotec) for astrocytes ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4, Miltenyi Biotec, Germany). All flow cytometry analyses were assessed using the following antibody clones ...
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bioRxiv - Immunology 2021Quote: ... CD14 cells were purified by anti-CD14 monoclonal antibody (mAb)-conjugated magnetic microbeads (Miltenyi Biotec) and then cultured for 6 days in RPMI 1640 medium (Life Technologies Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibodies were applied: anti-CD3-FITC (1:25, Miltenyi Biotec, #130-098-162), anti-CD14-FITC (1:25 ...
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bioRxiv - Genomics 2020Quote: ... anti-human EPCAM antibody conjugated to PE (1:50 dilution, Miltenyi Biotec #130-091-253), and either Calcein-AM (1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... the third aliquot containing 1 µL of CD45-VioBlue antibody (#130-110-802, Miltenyi Biotec); and the fourth aliquot containing 1 µL each of CD11b-APC antibody and CD45-VioBlue antibody ...
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bioRxiv - Neuroscience 2023Quote: ... the second aliquot containing 1 µL of CD11b-APC antibody (#130-113-793, Miltenyi Biotec); the third aliquot containing 1 µL of CD45-VioBlue antibody (#130-110-802 ...
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bioRxiv - Molecular Biology 2023Quote: ... CH12-F3 cells were stimulated with agonist anti-CD40 antibody (0.5μg/ml; Miltenyi Biotec; FGK45.5), mouse IL-4 (5 ng/μl ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with micro-magnetic beads conjugated Thy1.2 antibody (Miltenyi Biotec, 130-121-278) for 20 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... incubated with magnetic microbeads coupled to GFP antibodies (µMACS™ GFP Isolation Kit, Miltenyi Biotec) and complexes were eluted in 100µl of pre-warmed elution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with cardiac troponin T APC-conjugated antibody (Miltenyi Biotec, 130-120-403) or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec ...
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bioRxiv - Physiology 2024Quote: ... cells underwent magnetic-activated cell sorting using a CD56 antibody (130-050-401; Miltenyi Biotec), producing a CD56+ fraction consisting of muscle stem cells (MuSC ...
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bioRxiv - Microbiology 2024Quote: ... neutrophils were isolated via the use of a MACSxpress negative antibody collection kit (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Cytokines measured from PBMC supernatants were collected after 4 days of incubation with S protein and concentrations were predicted using a standard curve in the LEGENDplex assay (Miltenyi Biotec 741028).
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 150 ul of α-c-HA magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-094-255) as described above ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 30 µl of α-c-Myc magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-091-284) and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... 3 × 105 cells harvested from the spleen or cervical lymph nodes were incubated for 16 h in the presence of a peptide pool derived from the S protein of SARS-CoV-2 (a mixture of PepTivator SARS-CoV-2 Prot_S, S1, and S+; Miltenyi Biotec, Bergisch Gladbach, Germany). After washing the cells with PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant containing the resuspended proteins was used for immunoprecipitation assay using anti-GFP microbeads provided in the μMACS Epitope Tag Protein Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Approximately ...
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bioRxiv - Neuroscience 2024Quote: The assessment of expanded surface protein markers on sEV was carried out using a MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) following the manufacturer’s protocol.44 Thirty seven distinct surface marker antibodies (CD1c ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130-093-387 and 130-093-375) and IL-2 (100 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130- 093-387 and 130-093-375), and IL-2 (100 U/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then trypsinized and incubated with anti-CD146 antibody coated microbeads (Miltenyi Biotec Inc.) for 15 mins at 4°C to obtain the CD140b+CD146+ cells for subsequent experiments ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining (1 ×106 PBMC) was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were stained with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... where Magnetic isolation (MACS) was performed with anti-NG2 antibody conjugated with magnetic beads (Miltenyi Biotec). For the P9 time-point ...
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bioRxiv - Microbiology 2021Quote: ... The antibodies used for FACS analysis were APC-conjugated anti-CD163 (clone REA812, MACS Miltenyi Biotec), PE-conjugated mouse anti-HLA-DR (clone LN3 ...
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bioRxiv - Immunology 2022Quote: Separation was performed using anti-CD3 antibodies conjugated to ferromagnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and directed through a cell separation column containing a magnetic field (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2020Quote: ... Merkel cells were stained with anti-PSA-NCAM-PE antibody (Miltenyi Biotec, Cat. 130-117-394) or with mouse IgM-PE isotype control (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Isolated cells were incubated with fluorochrome conjugated antibodies ACSA-2-PE (1:50; Miltenyi Biotec, REA969), A2B5-488 (1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... Conjugated primary antibodies used was anti-cardiac troponin 2 (TNNT2) PE (Miltenyi Biotec – 130-120-405). Viability marker Zombie Aqua (Biolegend – 423102 ...
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bioRxiv - Neuroscience 2024Quote: Purified single-cells suspensions were stained using antibodies against mouse CD45 (Miltenyi Biotec; 130-117-498), SCA1 (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... streptavidin magnetic beads were first conjugated with the desired biotinylated antibody (anti-CD63, biotin; Miltenyi Biotec) and allowed to incubate with the sample stirring overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The pellets were resuspended in 50 μL of pooled REAfinity antibodies (Miltenyi Biotec, 1:50 dilution) for 40 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were MACs sorted using a biotinylated c-myc antibody (Miltenyi Biotec, 130-124-877) and isolated using the MiniMACS separator and columns (Miltenyi ...
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bioRxiv - Cancer Biology 2024Quote: ... and analyzed with an APC-conjugated anti-His monoclonal antibody (mAb, Miltenyi Biotec, Bergisch Gladbach, Germany) by flow cytometry ...
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bioRxiv - Neuroscience 2024Quote: ... The monocyte population was enriched using a human magnetic antibody cell sorting (MACS) system (Miltenyi Biotec), according to the manufactureŕs procedure ...
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bioRxiv - Genetics 2024Quote: ... A 2x antibody mix containing Anti-TRA-1-60-PE (Miltenyi Biotec, Cat.120-007-552) Alexafluor 488 Oct4 (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was conducted using anti-TNFα-APC and anti-IFN-γ-PE antibodies (Miltenyi Biotec). A VioBlue-live-dead marker (Thermo Fisher ...