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Citations for Miltenyi Biotec :
201 - 250 of 632 citations for Recombinant Human ACVR2A Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was performed on 500µl blood samples from healthy donors with or without Fc fragment receptor blockers (Miltenyi Biotec). Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... FITC/PE anti-human REAfinity™ TCR Vδ1 (REA173) and APC anti-human REAfinity™ TCR Vδ2 (REA771) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany); PE/Cy7 anti-mouse FasL (MFL3) ...
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bioRxiv - Microbiology 2023Quote: ... CD14+ cells were isolated from the PBMCs using human CD14+ micro beads conjugated with anti-human CD14 mAbs (130-118-906, Miltenyi Biotec, Bergisch Gladbach, Germany) LC columns (130-042-401 ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... and Vioblue anti-human CD45 antibodies (REA747, #5180719178 Miltenyi Biotec) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... incubated with human and mouse FcR blocking reagents (Miltenyi Biotec) for 15min ...
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bioRxiv - Microbiology 2022Quote: ... PE-Vio770-conjugated anti-human CCR5 (clone REA245, Miltenyi Biotec), PE-conjugated anti-human CD11b (clone REA713 ...
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bioRxiv - Microbiology 2022Quote: ... and CD81 antibodies (Human Exosome Isolation Kit, Pan, Miltenyi Biotec) followed by EV isolation via magnetic separation and elution ...
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bioRxiv - Genetics 2022Quote: ... Then cells were incubated with human CD34 Microbeads (Miltenyi Biotec) and Fc receptor blocker in MACS buffer (1% BSA/2mM EDTA/PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... we used CD45-VioBlue (human antibody, clone REA747, Miltenyi Biotec) to identify immune cells and in few cases ...
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bioRxiv - Genetics 2021Quote: ... Human CD14+ monocytes Isolation kit (Miltenyi Biotec, 130-050-201) and Human CD19+ B Cell Isolation kit (Miltenyi Biotec,130-050-301 ...
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bioRxiv - Molecular Biology 2021Quote: ... PBMCs were incubated with anti-human CD14 MicroBeads (Miltenyi Biotec) and monocytes were separated on a LS magnetic column on a QuadroMACS separator (Miltenyi Biotec) ...
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bioRxiv - Genomics 2021Quote: ... and APC anti-human CD99 (Miltenyi Biotec, #130-121-096). Cells were incubated for 15 min on ice and protected from light ...
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bioRxiv - Genomics 2021Quote: ... OctoMACS separator and CD19 MicroBeads (human) (all from Miltenyi Biotec) modifying slightly the data sheet protocol according to the experimental needs and the cell types (figure 1).
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bioRxiv - Systems Biology 2023Quote: ... Monocytes were positively selected using Human CD14 MicroBeads (Miltenyi Biotec) and LS Columns ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-human GLAST (ACSA-1) biotinylated antibody (Miltenyi Biotec, Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 100 U/ml human IL-2 (Miltenyi Biotec) and 100 U/ml Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T Cell TransAct (Miltenyi Biotec t# 130-111-160) was used to activate T cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... after treatment with a human FcR blocking reagent (Miltenyi Biotec). Flow cytometric analysis was performed as described above ...
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bioRxiv - Molecular Biology 2024Quote: A MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) was used ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of human FcR blocking reagent (Miltenyi Biotec) and subsequent staining with anti-PE paramagnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec). Cell purity was verified by flow cytometry ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Biochemistry 2021Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... and rinsed once with PBS and blocked in blocking buffer (PBS with 50% FCS, FcR Blocking Reagent 1:150 (Miltenyi Biotec) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were enzymatically dissociated using Human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s protocol (https://www.miltenyibiotec.com/AU-en/products/macs-sample-preparation/tissue-dissociation-kits/tumor-dissociation-kit-human.html#gref) ...
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bioRxiv - Neuroscience 2022Quote: ... human BDNF (100 ng/mL, MACS Miltenyi Biotec, 130-096-286), LM22A4 (5 µM ...
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bioRxiv - Immunology 2022Quote: ... using the human CD4+ T cell isolation kit (MACS Miltenyi Biotec). Isolated T helper cell purity was checked by flow cytometry CD4 staining and they were then cultured in CD3 (5µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). Ten micromolar integrase inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng/ml human M-CSF (130-096-491, Miltenyi Biotec), 10 ng/mL GM-CSF (130-093-862 ...
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bioRxiv - Immunology 2020Quote: Human nTregs were CFSE labelled and cultured in TexMACs (Miltenyi Biotec) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Systems Biology 2019Quote: ... antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec) at 4°C for 15 mins ...
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bioRxiv - Cancer Biology 2019Quote: ... The tissues were dissociated with Human Tumor Dissociated kit (Miltenyi Biotec) and filtered using 100µm SmarterStrainers (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... incubated with human FcR Block (Miltenyi Biotec, Cat#130-059-901) at 1/70 in PBS-FBS 1% for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Mouse anti-human PE anti-CD107a mAb (REA792, from Miltenyi Biotec) was added and cells were cultured at 37°C for one hour ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC with human CD14 MicroBeads (Miltenyi Biotec) MACS isolation using manual separation with LD columns (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). All HTLV-1 infected T-cell clones were maintained in 10 µM of the integrase inhibitor ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Primary cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and mouse-derived samples were additionally blocked with mouse FcR-blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Monocytes were magnetically sorted using human CD14+ microbeads (Miltenyi Biotec, USA) according to manufactures protocol ...