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Citations for Miltenyi Biotec :
201 - 250 of 1240 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Primary murine naïve CD4 T cells were isolated from the spleens and lymph nodes of SLE1.2.3 or control mice using the CD4+CD62L+ T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). CD62L positive selection was routinely ∼98% in purity as determined by flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... a mouse anti-human CD 90-FITC and a mouse anti-human CD73-APC (Miltenyi Biotec, Germany).
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bioRxiv - Plant Biology 2019Quote: ... membranes were incubated overnight with an anti-GFP-HRP antibody (130-091-833, dilution 1:1000, Miltenyi Biotec). For FLAG detection ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with PE-conjugated CD133 antibody (1:50 dilution; Miltenyi Biotec cat. no. 130-113-108) and PerCP-Cy5.5-conjugated CD271 antibody (1:50 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with anti-cytochrome c-APC antibody (Clone#REA702, 1:50) (Cat#130111180, Miltenyi Biotec). Samples were analyzed on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Pathology 2019Quote: ... MDSC and nonMDSC fraction were sorted using biotin-conjugated anti-Gr-1 antibody and streptavidin microbeads (Miltenyi Biotec) based on manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in PBS and stained with anti-CD133 antibodies conjugated with APC fluorophore (Miltenyi Biotec 293C3, 1:50) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Next PBMCs were then activated using 1 µg/mL immobilized CD3 and CD28 antibodies (Miltenyi Biotec, Cambridge, MA). After 3 days ...
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bioRxiv - Neuroscience 2021Quote: ... 4 °C and stained with the APC-labelled ACSA-2 antibody (1:10, Miltenyi Biotec, 130-102-315) in 0.5 % BSA in PBS ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany] ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mg of extract was incubated with µMACS MicroBeads conjugated to an anti-HA monoclonal antibody (Miltenyi Biotec) and beads were then captured on µMACS M-colums ...
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bioRxiv - Developmental Biology 2023Quote: ... germ cells were recovered using an anti-SSEA-1 antibody conjugated to MicroBeads (Miltenyi Biotec, 130-094-530) in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were incubated with APC-conjugated cytochrome c antibody (1: 50 dilution in permeabilisation buffer)(Miltenyi Biotec) and analyesd by FACS on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 minutes at 4°C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... primary ACC from the mouse was dissociated as single cells using the mouse Tumor Dissociation Kit (Miltenyi Biotec). Cells were then serially transplanted into NSG mice for approximately 6 months ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Samples and relevant controls were added with 2 μL of crystallizable fragment receptor blocker (Miltenyi Biotec, cat # 130-092-575) and incubated in the dark with rotation (12min ...
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bioRxiv - Cancer Biology 2023Quote: ... macrodissected HCC nodules and control liver samples were dissociated as single-cell suspensions using the Liver Dissociation kit (Miltenyi Biotec) and the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... mouse (130-092-613) from Miltenyi Biotec GmbH ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-mouse-IgG1 microbeads (Miltenyi Biotec). Naive and germinal center B cell purity (>90% ...
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bioRxiv - Immunology 2020Quote: ... anti-mouse IgG magnetic beads (Miltenyi Biotec) were added ...
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bioRxiv - Immunology 2022Quote: ... mouse” (Miltenyi Biotec Inc, Auburn, CA, USA) were used according to the protocol suggested by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... with CD11c MicroBeads UltraPure mouse (Miltenyi Biotec). CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... mouse (Miltenyi Biotec, Cat: 130-091-041) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then co-incubated with 1 μg of APC-conjugated anti-IgG antibody (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 μg of PE-conjugated anti-Flag antibody (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: ... 30 μg was detected with horseradish peroxidase (HRP)-conjugated antibodies against GFP (Anti-GFP-HRP, 1:10000, Miltenyi Biotec) or anti-tubulin (mouse monoclonal ...
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated with the surface antibody CD34-PE-Vio770 (clone AC136, lot #5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... cells were surface-labelled with an APC-conjugated anti-HA antibody (Miltenyi Biotec, Cat# 130-098-404, 1:50) and sorted by flow cytometry ...
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bioRxiv - Neuroscience 2023Quote: ... antibody labeling and boosting step was performed with the addition of CD11b (1:50) (Miltenyi Biotec, 130-110-611) and GFP-Nanobooster LOT 102 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... added with 40μL of magnetic microbeads conjugated with anti-Thy-1 antibody (Miltenyi Biotec 130–049–101, Auburn, CA), and mixed well ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec #130-091-833), anti-plant actin mouse monoclonal antibody (3T3)-HRP (Abbkine # A01050HRP) ...
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bioRxiv - Bioengineering 2023Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Immunology 2022Quote: ... Mouse hepatic parenchymal cells were digested and non-parenchymal cells were isolated by Mouse Liver Dissociation Kit (Miltenyi Biotec). Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse CAFs were separated from human pancreatic UM2 cancer cells using the Mouse Cell Depletion Kit (MACS Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Germany), resuspended at 106 cells/ml ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Cell Biology 2022Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
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bioRxiv - Evolutionary Biology 2022Quote: ... these control populations were measured after at 24 and 48 time periods using the FSC channel of a Macsquant10 (Miltenyi biotec). Flow cytometry was performed with the same setting used in pairwise fitness assays (see above) ...
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bioRxiv - Cell Biology 2024Quote: ... to serve as a control (i.e., unstimulated cells) or interleukin- 1β (IL-1β; 50ng/mL; Miltenyi Biotec, Cat#130-101-682) plus interferon-γ (IFNγ ...
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bioRxiv - Pathology 2024Quote: ... Isotype controls or fluorescence minus one (FMO) controls were used, and the live/dead marker 7-aminoactinomycin D (7-AAD, PerCP) (Miltenyi Biotec) was added to all samples 10 min before analysis (10 μl to 1 ml of cell suspension).
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bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
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bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
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bioRxiv - Systems Biology 2021Quote: ... these mice were euthanized and the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (130-104-075, Miltenyi Biotec), resuspended in a concentration of 106/ml ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Cancer Biology 2023Quote: Tumor samples harvested from mouse mammary glands were dissociated using the Mouse Tumor Dissociation Kit (Miltenyi Biotec,130-096-730), gentleMACS C tubes (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Immunology 2021Quote: ... Mouse FRCs required sorting using MACS (Miltenyi Biotec), with anti-mouse CD45 and CD31 magnetic beads ...