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Citations for Miltenyi Biotec :
201 - 250 of 1513 citations for Goat Anti Mouse IgG Fc FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... followed by incubation with anti-mouse FcR blocking reagent (130-092-575, Miltenyi Biotec) in PBS 2% FBS for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Primary resting mouse splenic B-cells were isolated using anti-CD43 microbeads (Miltenyi Biotec) and activated with 25 ug/mL LPS (Sigma) ...
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bioRxiv - Immunology 2024Quote: Resting B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was examined by flow cytometry using Annexin V-FITC Kit (Miltenyi Biotec, Italy), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Remaining cells were stained with 10 µl CD45-FITC (Miltenyi Biotec, 130-080-202) and 1 µl of Hoechst stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... and fluorescein isothiocyanate (FITC)-conjugated LNGFR antibody (Cat# 130-112-605, Miltenyi Biotec, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
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bioRxiv - Microbiology 2021Quote: ... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
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bioRxiv - Immunology 2020Quote: ... with 20% FCS or a whole skin digestion kit from Miltenyi Biotec (Cambridge, U.K.). Single cell suspensions were obtained and subsequently stained with the following antibodies against ...
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bioRxiv - Microbiology 2023Quote: ... Recovered cells were incubated with Fc block (Miltenyi Biotec, Bergusch Gladbach, Germany, 1:100), vortexed ...
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bioRxiv - Immunology 2020Quote: ... and IgG APC IS11-3B2.2.3 (Miltenyi Biotec). Data of labelled cells were acquired using a Beckman Coulter Gallios instrument and FlowJo ™ software (BD ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Systems Biology 2019Quote: ... cell suspensions were stained with anti-mouse CD45-PE-Vio770 (Miltenyi Biotec, 130-110-661), CD326-BV421 (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812, MACS Miltenyi Biotec), 0.14 µg/mL PE-conjugated mouse anti-HLA-DR (clone LN3 ...
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bioRxiv - Immunology 2021Quote: Lung epithelial cells separated by magnetic bead: the anti-mouse CD45 magnetic bead (Miltenyi Biotec) was used to enrich the epithelial cells in single-cell suspension ...
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bioRxiv - Immunology 2022Quote: ... mouse splenocytes were subjected to ACK lysis followed by anti-CD4 positive selection (Miltenyi Biotec). Naive CD4+ T cells (CD4+CD25-CD44-CD62L+ ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... + 5 µL DNase + 0.5% heat-inactivated FCS) with 10 µL FcR blocking reagent (Miltenyi Biotec), 10 µL biotin labeled anti-CD45 antibody (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... the leukocyte fraction was enriched for CD8+ T cells using anti-mouse CD8a microbeads (Miltenyi Biotec) and autoMACS equipment (Miltenyi Biotec ...
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bioRxiv - Genetics 2020Quote: ... CD43 positive cells were first depleted using anti-mouse CD43 (Ly-48) MicroBeads (MACS Miltenyi Biotec). Donor derived cells were then isolated by FACS sorting using fluorochrome-labelled antibodies CD45.2-FITC ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with anti-mouse CD45 MicroBeads and subjected to negative depletion (Miltenyi Biotec Inc). Cells were either used fresh for ex vivo experiments or preserved in CryoStor® CS10 freezing media (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... CD34+ cells were enriched via immunoselection using anti-mouse IgM microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and the anti-CD34 hybridoma clone 12.8 (a murine IgM anti-human CD34 cross-reactive with RM CD34) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 20 min on ice with anti-mouse ACSA2-APC (Miltenyi Biotec, USA). Astrocytes were sorted based on ACSA2 positive ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were subsequently stained with IL-17-FITC (1:20, rat, Miltenyi Biotec, 130-102-262), CD-3 APC/cy7 (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... We incubated with 5 µL of Annexin V-conjugated FITC (MACS Miltenyi Biotec, Lab Systems SA) at room temperature in darkness for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended and incubated in MACS buffer containing Fc blocker and CD11c MicroBeads (Miltenyi Biotec) according to manufacturer instruction ...
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bioRxiv - Developmental Biology 2019Quote: ... NeutrAvidin-functionalized devices were incubated with either (1) 2 μg/ml biotinylated mouse anti-human anti-SSEA-5 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) (2 ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were depleted of mature hematopoietic cells by anti-mouse antibody-labeled magnetic bead separation (Miltenyi Biotec) before fixation in formalin for 20 min at 4 °C.
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bioRxiv - Immunology 2020Quote: ... where mouse neutrophils were enriched from BM by positive magnetic selection with anti–CD11b microbeads (Miltenyi Biotec) before labelling with antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained with mouse anti-human CD19 antibody (Miltenyi Biotec, cat# 130-113-730; 1:50) in the dark for 25 minutes ...
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bioRxiv - Immunology 2019Quote: ... The diluted blood was labeled with antibodies directed against Ter119 (FITC, TER-119 1/30 Miltenyi Biotec), CD71 (PE ...
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bioRxiv - Immunology 2020Quote: ... each sample was divided into two tubes for either cytokine detection with IFN-γ-FITC (Miltenyi Biotec), IL-2-PE (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... and primary antibodies against CD45-FITC (12μl/sample, Cat nb. 130-114-567, Clone 5B1, Miltenyi Biotec), CD31-FITC (4μl/sample ...
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bioRxiv - Cancer Biology 2023Quote: ... To detect CD4, an antibody already labelled with FITC (130-113-775, clone REA623) from Miltenyi biotec was used at a concentration of 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Cell Biology 2021Quote: ... either MNCs or CD34 enriched cells were thawed and washed and stained with FC block (Miltenyi Biotec) for 10 min and washed again ...
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bioRxiv - Immunology 2023Quote: ... Livers sections were washed again and blocked using 1% Fc Block (MACS, Miltenyi Biotec, Auburn, CA, USA) and 5% BSA in HBSS during 1 hour at RT ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were purified from mouse bone marrow using anti-Ly6G MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). Purity checks and cell counts were performed using a BD Accuri C6 flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... and stained with 100 μl PE-conjugated mouse anti-human IgA (1:40; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...