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Citations for Miltenyi Biotec :
201 - 223 of 223 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cell Biology 2024Quote: ... The MicroBeads/cell extracts mixture was then applied to the μColumn followed by gentle wash three times with the lysis buffer used above for protein extraction (Miltenyi Biotec). Pre-heated (95°C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein purity was verified by protein gel electrophoresis followed by coomassie staining and western blotting using anti-His (HRP) antibody (Miltenyi Biotec). Signals were generated by chemiluminescence using Clarity ECL Western Blotting Substrate (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
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bioRxiv - Cell Biology 2024Quote: ... proteins from the native gel were transferred to the PVDF membrane and probed with anti-CD95 antibody (Miltenyi Biotec, clone DX2).
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Cytokines measured from PBMC supernatants were collected after 4 days of incubation with S protein and concentrations were predicted using a standard curve in the LEGENDplex assay (Miltenyi Biotec 741028).
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 150 ul of α-c-HA magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-094-255) as described above ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 30 µl of α-c-Myc magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-091-284) and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Microbiology 2022Quote: ... 3 × 105 cells harvested from the spleen or cervical lymph nodes were incubated for 16 h in the presence of a peptide pool derived from the S protein of SARS-CoV-2 (a mixture of PepTivator SARS-CoV-2 Prot_S, S1, and S+; Miltenyi Biotec, Bergisch Gladbach, Germany). After washing the cells with PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant containing the resuspended proteins was used for immunoprecipitation assay using anti-GFP microbeads provided in the μMACS Epitope Tag Protein Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Approximately ...
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bioRxiv - Neuroscience 2024Quote: The assessment of expanded surface protein markers on sEV was carried out using a MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) following the manufacturer’s protocol.44 Thirty seven distinct surface marker antibodies (CD1c ...
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bioRxiv - Immunology 2020Quote: The exosomes extracted from SVV-green fluorescence protein (GFP)-infected cells and normal cells were purified using an exosome purification kit (Miltenyi Biotec, Bergisch Gladbach, Germany), and the copy number of SVV was detected ...
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bioRxiv - Immunology 2022Quote: We stimulated the spleen and lung cells from immunized and control animals to detect nucleocapsid protein-specific CD8+ T cell responses with an N protein-peptide pool at a concentration of 1.5 μg/mL/peptide (Miltenyi Biotec; 130-126-699, Germany) at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029 ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...