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Citations for Miltenyi Biotec :
201 - 250 of 430 citations for 7 methyloct 3 en 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Immunology 2023Quote: ... and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec) and irradiated donor-mismatched PBMCs (all CAR-T cells ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10 ng/ml hFGF-2 (Miltenyi Biotec, 130-104-925). The media was further supplemented with 5µM CHIR99021 (StemMACS ...
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bioRxiv - Immunology 2022Quote: ... and 100IU/ml recombinant human IL-2 (Miltenyi Biotec, Bergisch Gladbach, Germany) were added ...
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bioRxiv - Neuroscience 2022Quote: ... microbeads containing antibodies directed against ACSA-2 (130-097-678, Miltenyi Biotec) were used.
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike PepTivator® protein pools (Miltenyi Biotec, Gladbach, GER) were used to test T cell responses against full spike proteome ...
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bioRxiv - Microbiology 2023Quote: ... 100 U/ml IL-2 (Miltenyi Biotec, Cat. No. 130-097-745) and 10 µM raltegravir (Selleck Chemicals ...
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bioRxiv - Microbiology 2023Quote: ... followed by IL-2 treatment (100U/mL Miltenyi Biotec 130-097-746). All experiments involving primary cells were performed at least 4 days post-activation.
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bioRxiv - Biochemistry 2023Quote: ... 2 μL of conjugated antibody (anti CD119-PE, Miltenyi Biotec cat # 130125874) or isotype control (IgG1-PE ...
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bioRxiv - Genomics 2024Quote: ... with 2 mL Nuclei Extraction Buffer (Miltenyi Biotec cat. #130-128-024) supplemented with 0.2 U/µL RNase Inhibitor (NEB cat ...
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bioRxiv - Neuroscience 2020Quote: ... single cell iPSCs were seeded at 3×105 cells/cm2 in StemMAC iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, DE) in presence of 10 µM Rock-Inhibitor Y-27632 (Cell Guidance Systems ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Neuroscience 2021Quote: ... and astrocytes magnetically labelled with ACSA-2 microbeads (Miltenyi Biotec, 130-097-678), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Livers were collected in 2 mL autoMACS Rinsing Solution + 0.5% BSA (Miltenyi Biotec). Tubes were then rocked for 30 min at room temperature to reduce epithelial cell proportions ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 µL of Cd45-VioBlue antibody (REA737, #130-110-802, Miltenyi Biotec). After mixing well ...
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745) for 2 days prior to flow cytometry analysis.
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745). HEK293FT cells were a gift from the Salk viral vector core and were cultured in DMEM (high glucose ...
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bioRxiv - Immunology 2020Quote: ... anti-CD8α (Ly-2)- and anti-CD90.2-MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany) and then separated over an auto-MACS column using the DepleteS program.
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bioRxiv - Immunology 2020Quote: ... by adding anti-CD45RA biotin (Miltenyi Biotec, Clone REA1047, 2 uL per stain) to the antibody cocktail ...
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bioRxiv - Neuroscience 2021Quote: ... 2 µL of Cd11b-APC antibody (M1/70, #130-113-793, Miltenyi Biotec), and 2 µL of Cd45-VioBlue antibody (REA737 ...
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bioRxiv - Genomics 2019Quote: Kidneys were dissociated using the Multi-tissue dissociation kit 2 from Miltenyi Biotec [130-110-203] as per manufacturers’ instruction ...
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bioRxiv - Neuroscience 2022Quote: ... and astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi Biotec). RNA-sequencing was performed using TrueSeq Stranded Total RNA V2 library preparation along with next-generation sequencing on the Illumina Novaseq 6000 platform with an approximate read-depth of 90 million reads per sample ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... gradient and cells were sorted using Anti-ACSA-2+ MicroBead Kit (Miltenyi Biotec). Cells were seeded onto 14 mm diameter glass coverslips in 24-well plates at a density of 20.000 cells/well for immunocytochemistry and in 6-well plates at a density of 500.000/well for qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... and APC-conjugated anti-ACSA-2 (Miltenyi Biotec, 130-116-245, 1:100) antibodies for 30 min at 4℃ ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured at 2×106 cells/ml in TexMACS medium (Miltenyi Biotec) supplemented with 10 ng/ml of IL-7 and IL-15 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 2×104 events were collected using a MACSQuant (Miltenyi Biotec) and analyzed using FlowJo v10.6.1 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Microbiology 2020Quote: ... in complete RPMI-1640 supplemented with 80 U/ml IL-2-IS (Miltenyi Biotec) and cultured in 96-well V-bottom plates ...
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bioRxiv - Cell Biology 2020Quote: ... Following astrocyte staining with ACSA-2-APC (Clone IH3-18A3, 1:200, Miltenyi Biotec), Hoechst to discriminate between cells and debris (10 µg/ml ...
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bioRxiv - Immunology 2020Quote: NK cells were expanded by culturing with IFN-α and IL-2 (Miltenyi Biotec) in cIMDM for 48 hours to induce priming ...
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bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
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bioRxiv - Immunology 2021Quote: ... then positively selected using anti-ACSA-2 MicroBeads (Miltenyi Biotec, Cat# 130-097-678). Total RNA was prepared using TRI Reagent (Millipore-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte ACSA-2+ cell sorting was performed using an autoMACS Pro Separator (Miltenyi Biotec). After isolation ...
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bioRxiv - Neuroscience 2023Quote: ... followed by ACSA-2 MicroBeads (cat # 130-097-678, MACS Miltenyi Biotec, Auburn, CA) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stimulated with IL-2 (10 ng/ml) (Miltenyi Biotec #130-097-745) and with anti-CD3/CD28 beads (Gibco #11132D ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-photon imaging was also performed with a TrimScope Matrix II from Miltenyi Biotec using an Olympus 25X W/NA 1.05 objective at a wavelength of 850 nm owing to Spectra Physics Insight X3+ laser.
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bioRxiv - Bioengineering 2024Quote: ... at a ratio of 1:2 in NK MACS media (Miltenyi Biotec, Gaithersburg, MD) supplemented with 5% human AB serum (Millipore Sigma ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Microbiology 2024Quote: Splenocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... The flow-through was pelleted at 1250 rpm in a swinging bucket centrifuge set to 6°C then resuspended in 3 ml cold AutoMACs Running Buffer (AMB, Miltenyi Biotec). After gentle resuspension ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...