Labshake search
Citations for Miltenyi Biotec :
201 - 250 of 458 citations for 7 Chloro quinazoline 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: Kidneys were dissociated using the Multi-tissue dissociation kit 2 from Miltenyi Biotec [130-110-203] as per manufacturers’ instruction ...
-
bioRxiv - Neuroscience 2022Quote: ... and astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi Biotec). RNA-sequencing was performed using TrueSeq Stranded Total RNA V2 library preparation along with next-generation sequencing on the Illumina Novaseq 6000 platform with an approximate read-depth of 90 million reads per sample ...
-
Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... gradient and cells were sorted using Anti-ACSA-2+ MicroBead Kit (Miltenyi Biotec). Cells were seeded onto 14 mm diameter glass coverslips in 24-well plates at a density of 20.000 cells/well for immunocytochemistry and in 6-well plates at a density of 500.000/well for qPCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... and APC-conjugated anti-ACSA-2 (Miltenyi Biotec, 130-116-245, 1:100) antibodies for 30 min at 4℃ ...
-
bioRxiv - Immunology 2024Quote: ... Cells were cultured at 2×106 cells/ml in TexMACS medium (Miltenyi Biotec) supplemented with 10 ng/ml of IL-7 and IL-15 (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2024Quote: ... A total of 2×104 events were collected using a MACSQuant (Miltenyi Biotec) and analyzed using FlowJo v10.6.1 ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec). All iPS lines were genotyped using an RFLP at the relevant important loci and the sequence was confirmed by Sanger sequencing ...
-
bioRxiv - Immunology 2021Quote: ... cut into 4 pieces and enzymatically digested by using the whole skin dissociation kit from Miltenyi biotec without enzyme P for 5 hours at 37°C under agitation (500 rpm) ...
-
bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
-
bioRxiv - Immunology 2021Quote: ... in the presence of 50 ng/ml GM-CSF and 20 ng/ml IL-4 (Miltenyi Biotec). Untouched peripheral blood cDC1 and cDC2 (cDCs ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec). Human cells were then cytospinned with Shandon cytofunnels (Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were washed with HBSS and spun at 300 g for 5min at 4 °C (Miltenyi Biotec). The pellet was resuspended and incubated with 80 µL of MACS buffer and 20 µL of anti-IgM microbeads (Miltenyi Biotec ...
-
bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
-
bioRxiv - Genetics 2019Quote: ... The mixture was incubated at 4°C for 30 min with 800 μL of Streptavidin MicroBeads (Miltenyi Biotec), which were then purified through an autoMACS Pro Cell Separator using the posseld2 program (UVA Flow Cytometry Core Facility) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... in complete RPMI-1640 supplemented with 80 U/ml IL-2-IS (Miltenyi Biotec) and cultured in 96-well V-bottom plates ...
-
bioRxiv - Cell Biology 2020Quote: ... Following astrocyte staining with ACSA-2-APC (Clone IH3-18A3, 1:200, Miltenyi Biotec), Hoechst to discriminate between cells and debris (10 µg/ml ...
-
bioRxiv - Immunology 2020Quote: NK cells were expanded by culturing with IFN-α and IL-2 (Miltenyi Biotec) in cIMDM for 48 hours to induce priming ...
-
bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
-
bioRxiv - Immunology 2021Quote: ... then positively selected using anti-ACSA-2 MicroBeads (Miltenyi Biotec, Cat# 130-097-678). Total RNA was prepared using TRI Reagent (Millipore-Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... astrocyte ACSA-2+ cell sorting was performed using an autoMACS Pro Separator (Miltenyi Biotec). After isolation ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by ACSA-2 MicroBeads (cat # 130-097-678, MACS Miltenyi Biotec, Auburn, CA) for 15 min ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were stimulated with IL-2 (10 ng/ml) (Miltenyi Biotec #130-097-745) and with anti-CD3/CD28 beads (Gibco #11132D ...
-
bioRxiv - Bioengineering 2024Quote: ... at a ratio of 1:2 in NK MACS media (Miltenyi Biotec, Gaithersburg, MD) supplemented with 5% human AB serum (Millipore Sigma ...
-
bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2-photon imaging was also performed with a TrimScope Matrix II from Miltenyi Biotec using an Olympus 25X W/NA 1.05 objective at a wavelength of 850 nm owing to Spectra Physics Insight X3+ laser.
-
bioRxiv - Microbiology 2024Quote: Splenocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
-
bioRxiv - Genetics 2020Quote: ... following the manufacturer’s instruction for 30 minutes (4 °C) before being passed successively through two LS Columns (Miltenyi Biotec) with three MACs buffer washes ...
-
bioRxiv - Microbiology 2020Quote: ... and the medium on the monocytes was replenished with X-vivo 15 + L-glutamine containing interleukin-4 (Miltenyi Biotec) and granulocyte-macrophage colony-stimulating factor (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2019Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
-
bioRxiv - Neuroscience 2021Quote: ... sliced into 4 sagittal sections and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec, San Diego, CA), and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937 ...
-
bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
-
bioRxiv - Cancer Biology 2022Quote: ... and 37°C incubator and passaged every 4-5 days in small clusters using StemMACS Dissociation reagent (Miltenyi Biotec). Colon organoids were differentiated as previously described[46] with minor modifications ...
-
bioRxiv - Physiology 2023Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
-
Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... at 4°C for 10 minutes and then isolated using MACs LS (Miltenyi Biotec; Catalog no. 130-042-401) columns following the manufacturer’s instructions ...
-
bioRxiv - Pathology 2019Quote: ... and blocked in FACS buffer (PBS, 2% BSA) containing an FcR blocking reagent (Miltenyi Biotec). Samples were stained with fluorophore-conjugated antibody cocktails ...
-
bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were washed twice with 1× PBS + 2% FBS and fed to autoMACS (Miltenyi Biotec) to enrich for CD117+ cells ...
-
bioRxiv - Immunology 2022Quote: ... Cells were seeded 2 or 3 days before transfection experiment by MACSfectin reagent (Miltenyi Biotec), to achieve cell density recommended by the supplier.
-
bioRxiv - Neuroscience 2022Quote: ... Astrocyte fraction was enriched using Anti-ACSA-2 MicroBead Kit (Miltenyi Biotec, #130-097-679).
-
bioRxiv - Neuroscience 2021Quote: ... cell pellets were resuspended in 1 mL FACS buffer (DPBS with 2% BSA, Miltenyi Biotec #130-091-376 ...
-
bioRxiv - Immunology 2020Quote: ... anti-Dectin-2 PE (REA1001) and anti-CD64 APC (REA286) were purchased from Miltenyi Biotec; rat anti-Dectin-2 (D2.11E4 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and stimulated for activation for 3 days supplemented with 20ng/μL IL-2 (Miltenyi Biotec) and CD3/CD28 Dynabeads Human T-Activator (Gibco ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by addition of 150 μL Anti-ACSA-2 microbeads (Miltenyi Biotec 130-097-678). The sample was placed on a rotating wheel at 4°C for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... and the membrane glycoprotein (PepTivator® SARS-CoV-2 Prot_M) were obtained from Miltenyi Biotec. The PepTivator® Peptide Pools are constituted by peptides of 15 amino acid length with 11 amino acid overlap ...
-
bioRxiv - Neuroscience 2023Quote: ... 2) 4.17 mM GadoSpin-P (n=6; MW=200 kDa; Miltenyi Biotec, Bergisch Gladbach, Germany); and 3 ...
-
bioRxiv - Neuroscience 2023Quote: ... pre-filled with 2 mL ice-cold Nuclei Extraction Buffer (#130-128-024, Miltenyi Biotec) supplemented with 0.2 U/μL RNase inhibitor (#2489605 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...