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Citations for Miltenyi Biotec :
201 - 250 of 554 citations for 7 Chloro 9 methyl 3 4 dihydro 2H benzo b oxepin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: B cells were purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec) and subsequently expanded for 10-14 days in RPMI-1640 supplemented with 10% human serum (Bavarian Red Cross) ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were neutralized with Ca2+/Mg2+ free DPBS with 0.5% BSA (Miltenyi Biotec, 130-091-376) and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... in combination with or without recombinant interleukin-4 (IL-4, Miltenyi Biotec, catalogue number: 130-094-061) for 24h ...
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bioRxiv - Immunology 2021Quote: ... The isolated T cells were then stimulated with IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany) at a concentration of 25μg/2L bag of TexMACS media with 3% human AB serum (Innovative Research ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 and 4 cytokines were added as followed: 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 100 ng/ml GM-CSF (#130-093-864 ...
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bioRxiv - Immunology 2021Quote: ... Naïve mature B cells were isolated from spleen cells by immunomagnetic negative selection with anti-CD43 (Miltenyi Biotec). B cells were cultured in RPMI-1640 medium (Corning ...
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bioRxiv - Cancer Biology 2020Quote: For CSR experiments spleen naive B cells were isolated by immunomagnetic depletion using anti-CD43 beads (Miltenyi Biotec). Purified naïve B cells were cultured at 1.2 x 106 cells/ml in complete RPMI medium supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD19+ B cells were magnetically removed from freshly isolated PBMCs using CD19 Microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s recommendations (depleted condition) ...
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bioRxiv - Molecular Biology 2021Quote: Nave B lymphocytes were purified from mouse spleens by negative selection using CD43 (Ly48) magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions. ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was achieved using the 37_C Multi_tissue_dissociation B program on the gentleMACS octo-dissociator (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... Total splenic B cells (CD19+) were purified using CD19 MicroBeads (130-121-301, Miltenyi Biotec, San Jose, CA), and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862 ...
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bioRxiv - Cell Biology 2022Quote: ... and B cells and CD8+ T cells were isolated from these using the human CD19 Microbeads (Miltenyi Biotec) or the human CD8+ isolation kit (Miltenyi Biotec).
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bioRxiv - Molecular Biology 2023Quote: Splenic naïve B-cells were prepared using the CD43 negative selection commercial kit (Miltenyi Biotec.; 130-090-862). Spleens were pulled from 2-3 sacrificed sex- and age- matched animals mechanically disintegrated and filtered through a 70 µm cell strainer to prepare a single cell suspension essential for microbead separation ...
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bioRxiv - Immunology 2023Quote: ... and B cells were isolated by negative selection directly from whole blood using MACSxpress® separator (Miltenyi Biotec) with Whole Blood B Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T lymphocytes and CD20+ B lymphocytes were sorted from PBMCs using CD3+ and CD20+ microbeads (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... Th2: 20ng/ml rmIL-4 (Miltenyi Biotec). Intracellular cytokine staining was performed after incubation for 4-6h with Cell Stimulation cocktail plus Golgi transport inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ng/mL BMP-4 (Miltenyi Biotec), and 10 µM Y-27632 (Abcam ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Molecular Biology 2022Quote: ... Plated neurospheres were maintained for 7 days on N2B27 basal medium supplemented with Dual Smad inhibitors (Miltenyi Biotec (SB), Tocris (DM)) ...
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bioRxiv - Genomics 2023Quote: ... TB6505 and TB6545 (1 givinostat and 7 vehicle slices) were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) with modifications ...
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bioRxiv - Neuroscience 2019Quote: ... and equilibrated with 3 mL Separation Buffer (Miltenyi Biotec). Microbead-coated mitochondria were applied onto the LS column ...
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bioRxiv - Immunology 2019Quote: ... 30ng/ml anti-CD3 antibody (OKT-3) (Miltenyi Biotec) and 3000IU/ml IL-2 ...
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bioRxiv - Developmental Biology 2022Quote: ... using the Multi Tissue Dissociation Kit 3 (Miltenyi Biotec), and pooled ...
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bioRxiv - Molecular Biology 2021Quote: Resting primary B lymphocytes (wild-type and Tp53bp1-/-) were purified from mouse spleens using anti-CD43 microbeads (Miltenyi Biotec). One million cells were stimulated to proliferate with a cytokine cocktail containing 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Genetics 2022Quote: ... Peripheral T- and B-cells were isolated from whole spleen using Pan T Cell (Miltenyi Biotec,130-095-130) or Pan B Cell (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Naïve B cells (CD43-B220+CD3-) were obtained by negative selection (mouse CD43 Ly-48 MicroBeads; MACS Miltenyi Biotec) from spleens of WT and Lrba-/- mice with 95% purity ...
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bioRxiv - Immunology 2019Quote: ... Either PBMC or B cells magnetically sorted using the AutoMACS Pro cell separator and CD19+ microbeads (both Miltenyi Biotec), were lysed in RLT buffer (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... B cells were first isolated by magnetic cell sorting using the human CD19 MicroBeads (Miltenyi Biotec, San Diego, CA) and the AutoMACS magnetic cell separator ...
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bioRxiv - Immunology 2022Quote: ... the complete sequence of B.1.1.7 D614G Spike mutant (alpha) (JPT Peptide Technologies) or wildtype (WT) S-protein (Miltenyi Biotec) or left untreated as a control for 16 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were filtered to remove dead cells and B-cell depletion was performed using human CD19 microbead (Miltenyi Biotec) labeling according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ splenic B cells from SWHEL donor mice were purified using anti-mouse CD43 (Ly-48) MicroBeads (Miltenyi Biotec). If not specified ...
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bioRxiv - Biophysics 2023Quote: ... and resting mouse B-cell isolation kit (Cat# 130-090-862) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Oligonucleotides were obtained from Integrative DNA Technologies (Coralville ...
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bioRxiv - Immunology 2023Quote: We prepared B cell tetramers of biotinylated S2hlx-Ex19 or FP-10 by mixing with Streptavidin-VB515 (Miltenyi Biotec) and Streptavidin-AlexaFluor 647 (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 1 × 10∧7 cells were processed for dead cell depletion using Dead Cell Removal Kit (Miltenyi Biotec # 130-090-101) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei pellets were washed tree times with one filtration step by MACS pre-separation filter 20μm (Miltenyi Biotec). Nuclei pellets were resuspended in 100 μL of staining buffer (DPBS BSA 2% ...
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bioRxiv - Immunology 2019Quote: ... NKM-PE (16-2-4) from Miltenyi Biotec, Vγ7-FITC (kindly provided by P ...
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bioRxiv - Systems Biology 2020Quote: ... and 250 IU/mL IL-4 (Miltenyi Biotec) on days 1 ...
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bioRxiv - Immunology 2020Quote: ... interleukin 4 (IL4) (Miltenyi Biotec, Bergisch-Gladbach, Germany) was added to some wells at 10 ng/ml.
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bioRxiv - Immunology 2021Quote: ... truncated hCD4 gene (pMACS 4-IRES.II; Miltenyi Biotec) and 3’ loxP site flanked with arms of homology to exon 5 of the Il22 gene was recombineered into RP24-227B3 (see Figure S1A) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 100ng/mL Human IL-4 (Miltenyi Biotec) for 5 days only adding media once in between ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50ng/mL Human IL-4 (Miltenyi Biotec). Monocytes were plated into 6-well plates (5×105 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 ng/ml anti-CD3 antibody (OKT-3) (Miltenyi Biotec) and 3000 IU/ml hrIL-2 ...
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bioRxiv - Cell Biology 2022Quote: ... and dissociated with MACS MultiTissue Dissociation Kit 3 (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 3) Astrocytic (ACSA2-APC (REA969, #130-116-245, Miltenyi Biotec)) ...
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bioRxiv - Immunology 2024Quote: ... 30 ng/ml anti-CD3 antibody (OKT-3) (Miltenyi Biotec) and 3000 IU/ml hrIL-2 as previously reported12 ...
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bioRxiv - Immunology 2023Quote: ... Cells were depleted of CD4+ T cells by staining with CD4 PE-conjugated antibody (RM4-4) for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec.) as described above ...
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bioRxiv - Immunology 2020Quote: Splenic B cells of both genotypes were CD43-depleted using magnetic beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany) and taken in culture in the presence of 1μg/ml anti-CD40 antibody (102901 ...
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bioRxiv - Microbiology 2021Quote: ... Non-T-cells containing monocytes and B-cells were prepared by T-cell depletion using anti-biotin MicroBeads (Miltenyi Biotec), biotin-labeled anti-CD4 mAb (OKT4 ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were depleted of B and NK cells via CD19+ and CD56+ microbeads according to the manufacturer’s protocol (Miltenyi Biotec). M1- and M2- macrophages were generated from blood monocytes in vitro ...