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Citations for Miltenyi Biotec :
2301 - 2350 of 2522 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Cell sorting was performed on Control and EIF4G2KD HEC-1A cells following magnetic beads separation of CD133+ and CD133-(CD133 Microbead Kit, Miltenyi Biotec, 130-097-049) and LS-columns (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... isolated mononuclear cells were positively selected for CD34 using the CD34+ Microbead kit Ultrapure (Miltenyi Biotec, San Diego, CA, USA, cat.: 130-100-453). Cells were cultured at 1.5×105–2.5×105 cells/mL in CellGenix® GMP Stem Cell Growth Medium (SCGM ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Cancer Biology 2024Quote: ... while the resulting Lin- (lineage-negative) fraction was then enriched for c-Kit expression using CD117 MicroBeads (Miltenyi Biotec, Catalog no: 130-091-224). These cKit-enriched cells were washed ...
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bioRxiv - Genomics 2024Quote: ... The cells were centrifuged at 300 x g for 10 minutes and further submitted to live cell sorting with the Magnetic Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101), according to the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated into a single cell suspension and the debris were removed using the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) and gentleMACS program 37C_ABDK_02 according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were transcardially perfused with cold PBS and cortical regions were quickly dissected and enzymatically digested using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) in the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: Freshly enriched EVs from WJ-MSC WT and CD81-Snorkel-tag cell supernatants were immunoprecipitated either with hCD81 exosome isolation kit (Miltenyi biotec; 130-110-914) or µMACS™ HA Isolation Kit (Miltenyi biotec ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected in free RPMI medium and then dissociated for single-cell suspension by the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells counts were determined by flow cytometer Novocyte (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... The diluted blood was labeled with antibodies directed against Ter119 (FITC, TER-119 1/30 Miltenyi Biotec), CD71 (PE ...
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bioRxiv - Cancer Biology 2019Quote: ... The transfected TI lymphocytes were stimulated with the anti-CD3 antibody (1 μg/mL, OKT3, Miltenyi Biotec) coated on the plate for 84 h ...
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bioRxiv - Cancer Biology 2022Quote: ... After several washing with 1×DPBS containing 0.04% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376), gastric tumor tissues single cells were acquired with digestive enzymes containing 200 μL Enzyme H ...
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bioRxiv - Neuroscience 2021Quote: ... aliquots of cells were stained with: 1) Microglial (Cd11b-APC (M1/70, #130-113-793, Miltenyi Biotec) / Cd45-Vioblue (REA737 ...
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bioRxiv - Cell Biology 2020Quote: ... ESCs culture medium consists of N2B27 medium (Life technology) supplemented with PD0325901 (PD, 1 μM, Miltenyi Biotec), CHIR99021 (CH ...
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bioRxiv - Cell Biology 2021Quote: ... dissociated cells were incubated with CD90 microbeads (Miltenyi Biotec, 130-096-253; 1 μl per 107 cells) to enrich CD90+ NPCs.
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bioRxiv - Developmental Biology 2021Quote: ... and positively selected using 10 uL PDGFRα antibody-coated microbeads (1:10; Miltenyi Biotec #130-101-547). PDGFRα+ stoma was placed into magnetic separation MS columns (Miltenyi Biotec #130-042-021 ...
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bioRxiv - Immunology 2022Quote: ... Cells were resuspended in PBS containing 1% FBS and incubated with mouse FcR blocking reagent (Miltenyi Biotec) for 10 minutes at 4°C before antibody staining with 1:50 dilution for 30 minutes at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Livers sections were washed again and blocked using 1% Fc Block (MACS, Miltenyi Biotec, Auburn, CA, USA) and 5% BSA in HBSS during 1 hour at RT ...
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bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Neuroscience 2020Quote: ... brain stem and cerebellum were removed and the remaining cerebrum was freed from meninges and dissociated by enzymatic digestion using the Neural Kit P (Miltenyi Biotec; Cat#130-092-628). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... CD8 T cells were isolated from single-cell splenocyte suspensions using the Miltenyi CD8a+ T cell isolation kit (Miltenyi Biotec, Inc. Cat.130-104-075) according the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Intestinal lymphocytes were isolated from RAG1-/- recipients using a lamina propria dissociation kit according to the manufacturer’s instructions (Miltenyi Biotec, Cat. No.: 130-097-410). Briefly ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow was flushed from femurs and tibias and HSCs were isolated by negative selection with the Direct Lineage Depletion Kit (Miltenyi Biotec, Cat#130-110-470) using MACS technology ...
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bioRxiv - Developmental Biology 2022Quote: ... The right ventricle was perfused with ice cold PBS and the lungs were harvested quickly and homogenized and dissociated using the Miltenyi MACS lung dissociation kit (Miltenyi Biotec, cat # 130-095-927), using the GentleMacs program m-Lung-1 on the AUTO MaCs ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi tissues were dissociated to single-cell suspension by enzymatic degradation using a MACS Technology neural tissue dissociation kit (Miltenyi Biotec, Cat# 130-092-628) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Hearts were cut in small pieces and dissociated at 37°C with the commercially available enzyme mix (Neonatal Heart Dissociation Kit, mouse and rat, Miltenyi Biotec GmbH; # 130-098-373) followed by tissue homogenization with the genteMACS™ Dissociator (Miltenyi Biotec GmbH ...
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bioRxiv - Immunology 2023Quote: Spleens from four- to six-week-old mice were processed to single cell suspensions and CD4+ T cells were enriched using mouse CD4 (L3T4) Microbeads kit (Miltenyi Biotec Cat # 130-117-043) according to manufacturer instructions ...
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bioRxiv - Pathology 2023Quote: Splenic CD4 T cells from WT mice were enriched using naive untouched CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec, cat. No. 130-104-453). Isolated CD4 T cells (1 x106 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... The right ventricle was perfused with ice cold PBS and the lungs were harvested quickly and homogenized and dissociated using the Miltenyi MACS lung dissociation kit (Miltenyi Biotec, cat # 130-095-927), using the GentleMacs program m-Lung-1 on the AUTO MaCs ...
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bioRxiv - Cell Biology 2023Quote: Murine nCMs were isolated from the ventricles of 1-2 days old wildtype C57BL/6 and Lmnaflox/flox mouse pups using MACS neonatal heart dissociation kit according to the manufacturer (Miltenyi Biotec cat# 130-098-373). Following digestion ...
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bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was incubated with RBC lysis buffer for 5 min on ice to remove red blood cells (RBC) and then used for Treg isolation with a CD4+CD25+ Treg isolation kit (Miltenyi Biotec, cat # 130-091-041), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were incubated overnight with an anti-GFP-HRP antibody (130-091-833, dilution 1:1000, Miltenyi Biotec). For FLAG detection ...
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bioRxiv - Cell Biology 2020Quote: ... Stro-1 positive cells were collected by magnetic-activated cell sorting (MACS) according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... Right superior and inferior lobes were homogenized in 1 ml sterile PBS using gentleMACS Octo Dissociator (Miltenyi Biotec). A 100 μl aliquot of homogenate was reserved for bacteria cfu assay ...
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bioRxiv - Immunology 2021Quote: ... or overnight with 1 μg/peptide per well of spike associated (Cat: 130-127-951, Miltenyi Biotec Peptivator) peptides at 37°C ...
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bioRxiv - Genetics 2021Quote: Fresh G-CSF mobilized peripheral blood cells from healthy donor 1 were obtained from Miltenyi Biotec (Auburn, CA). CD34+ HSPCs were isolated using CliniMACS® CD34 reagent (Miltenyi ...
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bioRxiv - Cancer Biology 2022Quote: ... and then blocked in FACS buffer (PBS, 2% BSA) containing FcR blocking reagent at 1:50 (Miltenyi Biotec) for 15 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with PE-conjugated CD133 antibody (1:50 dilution; Miltenyi Biotec cat. no. 130-113-108) and PerCP-Cy5.5-conjugated CD271 antibody (1:50 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with anti-cytochrome c-APC antibody (Clone#REA702, 1:50) (Cat#130111180, Miltenyi Biotec). Samples were analyzed on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Pathology 2019Quote: ... MDSC and nonMDSC fraction were sorted using biotin-conjugated anti-Gr-1 antibody and streptavidin microbeads (Miltenyi Biotec) based on manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in PBS and stained with anti-CD133 antibodies conjugated with APC fluorophore (Miltenyi Biotec 293C3, 1:50) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... EpSCs were isolated using magnetic-associated cell sorting using Anti-Sca-1 microbeads (Miltenyi Biotec, 130-106-641) and a MACS MultiStand system (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... in C tubes and then processed using program 37C_m_LDK_1 (for LUAD tumors) or 37C_m_TDK1_1 (for PDAC tumors) on a gentleMACS Octo dissociator with heaters (Miltenyi Biotec). Spleens were placed in 3 ml of PBS supplemented with 2% FBS in C tubes and dissociated using program m_spleen_01 on a gentleMACS Octo dissociator with heaters (Miltenyi Biotec) ...