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Citations for Miltenyi Biotec :
2201 - 2250 of 2473 citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Primary NK cells were enriched from normal human peripheral blood mononuclear cells (PBMCs) (Zenbio Inc.) using NK cell isolation kit (Miltenyi Biotec, 130-092-657). The purity of isolated NK cells was confirmed by staining of APC conjugated anti-human CD56 antibody and PE conjugated anti-human CD16 antibody ...
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bioRxiv - Bioengineering 2022Quote: The cell pellet was then incubated in a 4 °C fridge with rocking for 15 minutes in 100 µL of MACS buffer and 25 µL of lineage depletion microbeads from the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Mononuclear cells were isolated by density gradient centrifugation and CD34+ cells were subsequently isolated using the MACS CD34 MicroBead kit and the autoMACS (Miltenyi Biotec, Amsterdam, The Netherlands). CB-MLL-AF9 (here mBlueberry2 ...
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bioRxiv - Cancer Biology 2019Quote: Half of each tumor was dissociated using the Human Tumor Dissociation Kit and Gentle MACS Tissue Dissociator with Heaters (Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s instructions prior to enumeration of live cells using trypan blue.
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bioRxiv - Immunology 2020Quote: ... The supernatant was removed and the pellet resuspended and processed as directed by the Multi-tissue Dissociation Kit I (Miltenyi Biotec, 130-110-201). Brains were enzymatically digested into a single cell suspension by rotating at 37°C for approximately 25 minutes with trituration halfway through incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Gt(ROSA26)SorCreERT2/+ mice as described (Hatsell et al., 2015)for tamoxifen regime using the Skeletal Muscle Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: Brain tissue samples including the cortex and hippocampus obtained from experimental mice that did or did not receive the mCRP treatment were gently dissociated into single-cell suspensions using the Adult Brain Dissociation kit (#130107677, Miltenyi Biotec, Auburn, CA, USA). Single-cell isolation and characterization of CD31+ cells were performed as previously described with a minor modification 10 ...
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bioRxiv - Bioengineering 2021Quote: ... The Lin− fraction of the CB cells and the AML cells were further enriched for CD34 with the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, 130-100-453), which contains microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected into single lobes before being dissociated into single cell suspension using gentleMACS octo-dissociator and Miltenyi lung dissociation kit (Miltenyi Biotec, 130-095-927). Red blood cells (RBCs ...
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bioRxiv - Bioengineering 2021Quote: ... and was then dissociated with an enzymatic and mechanical method using a MACS Neural Tissue Dissociation Kit for postnatal neurons (Miltenyi Biotec, 130-094-802) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... Lung was collected in RPMI and was dissociated into single-cell suspension using the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) as per the company’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Fully de-identified human CD34 cells enriched blood samples were obtained from Bone Marrow and Transplantation Laboratory at UCSF and were MACS sorted using CD34 MicroBead Kit (Miltenyi Biotec, 130-046-702) and incubated with CD3 antibody (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: Purified milk and serum EVs were subjected to multiplex bead-based analysis by flow cytometry using the human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813). EV samples were processed overnight in the MACSPlex Filter Plate as described in the manufacturer’s instructions and in a previously published paper on a systematic evaluation of the assay19 ...
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bioRxiv - Genomics 2022Quote: 3ug of biotinylated RNA and 60pg of in vitro transcribed spike-in RNA (ERCC-00048) in 200ul water was mixed with 100ul of beads from the uMACS Streptavidin kit (Miltenyi Biotec 130-074-101) on a thermoblock at 24°C at 800 RPM for 15 minutes ...
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bioRxiv - Immunology 2022Quote: Splenic B cells from 24-week old MRL/lpr mice were isolated with a Pan B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). B cells were cultured with RPMI-1640 medium (Lonza ...
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bioRxiv - Physiology 2023Quote: Single cardiomyocytes were isolated from the ventricles of C57BL/6J WT mouse neonates aged from postnatal day 0 to 3 by enzymatic and mechanical dissociation in a semi-automated procedure by using the Neonatal Heart Dissociation kit and the GentleMACS™ dissociator (Miltenyi Biotec, Gaithersburg, MD). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Systems Biology 2022Quote: Naïve CD8+ T cells were isolated from the peripheral blood of two healthy donors using the Naive CD8+ T Cell Isolation kit (Miltenyi Biotec, 130-093-244) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The tissue pieces remaining after enzymatic treatment and filtration of the fresh sample were processed using a human tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions to obtain a single-cell suspension ...
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bioRxiv - Cell Biology 2023Quote: MuSCs were isolated from the hindlimb muscles of 11–13-week-old mice following MACS using a Satellite Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and anti-integrin α7 microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... Tregs were isolated from fresh PBMCs by magnetic separation using the CD4+CD25+CD127dim/- human Treg isolation kit (Miltenyi Biotec, Cat: 130-094-775). Tregs were then used directly for analysis or assays.
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bioRxiv - Immunology 2024Quote: ... The remaining submucosal tissue was placed in a conical tube containing fresh RPMI-1640 supplemented with digestion enzymes from the human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) and chopped into small pieces using dissection scissors ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were harvested 48 h post-electroporation and dissected brains were treated with Neural Tissue Dissociation Kit P (Macs Miltenyi Biotec, 130-092-628). Genomic DNA from FAC-sorted RFP+ cells upon in-utero electroporation was obtained by Qiagen QIAmp DNA Minikit (# 157050681 ...
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bioRxiv - Bioengineering 2024Quote: ... The cell pellet was resuspended in 80 µL of FACS buffer and 20 µL of the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268). The cells were incubated for 15 min at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: For primary cardiac myofibroblast enriched cultures cells were first isolated from adult mice hearts using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) and gentleMACS homogenizer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic cell dissociation and debris and red blood cell removal were performed using the Adult Brain Dissociation Kit (130-107-677; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T lymphocytes were purified from total PBMC by Naïve CD8+ T Cell Isolation Kit and MACS® separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Primary CD4+ T lymphocytes were isolated from peripheral blood mononuclear cells and purified by negative selection using the CD4+ T cell isolation kit (Miltenyi Biotec, 130-096-533). Cells were phenotypically analyzed and activated using anti- CD3/antiCD28 coated plates (Ref BE0001-2 and Ref BE0248 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched CD4+ T cells were stained with fluorescent antibodies against CD4 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
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bioRxiv - Immunology 2023Quote: To assess immune cells and endothelia from the lung left lobe of lung was digested using a GentleMACS lung digestion kit (Miltenyi Biotec #130-095-927). In brief ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of splenocytes from individual mice were prepared and B cell enrichment was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). Plasmablast cell sorting was performed using a FACSAria (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... and later monocytes were enriched from the total bone marrow cells using the Monocyte Isolation Kit (BM) from Miltenyi Biotec (Cat: 130-100-629) through a negative selection process following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... dead cells were removed from the culture via a Miltenyi Biotec Dead Cell Removal Kit and MiniMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Untouched T cells were isolated from PBMCs by negative Magnetic-activated cell sorting (MACS) using the human pan T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The ventricles were sectioned into small cubes and processed using mechanical dissociation (gentleMACS) and enzymatic degradation (neonatal heart dissection kit; Miltenyi Biotec, Bergisch Gladbach, Germany). The cell suspension was then filtered and the cells were plated onto glass-bottom dishes (MatTek Corp. ...
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bioRxiv - Microbiology 2023Quote: ... immune cells from the colonic lamina propria were extracted using the Lamina Propria Dissociation Kit for mice and gentleMACS dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Cancer Biology 2023Quote: We isolated human umbilical cord blood (hUCB) CD34+ cells from two patients using the Miltenyi CD34 Microbead Kit and MACS separation column (Miltenyi Biotec, Boston, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cell sorting was performed on Control and EIF4G2KD HEC-1A cells following magnetic beads separation of CD133+ and CD133-(CD133 Microbead Kit, Miltenyi Biotec, 130-097-049) and LS-columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... isolated mononuclear cells were positively selected for CD34 using the CD34+ Microbead kit Ultrapure (Miltenyi Biotec, San Diego, CA, USA, cat.: 130-100-453). Cells were cultured at 1.5×105–2.5×105 cells/mL in CellGenix® GMP Stem Cell Growth Medium (SCGM ...