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Citations for Miltenyi Biotec :
2201 - 2250 of 2886 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens by using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... CD8+ T cells were isolated by negative selection using magnetic beads following the instructions from the manufacturer (Miltenyi Biotec). The purified CD8+ T cells were incubated with APC-conjugated HLA-A2/YFV NS4b (LLWNGPMAV ...
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bioRxiv - Cell Biology 2022Quote: ... complete DMEM (with FBS) was added to the cell suspension and sorted using MACS LS columns (Miltenyi Biotec GmBH). The columns were washed two times with 1 ml of cold DMEM each ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor and matched normal samples were dissociated into single cell suspension using human tumor dissociation kit (Miltenyi Biotec) and gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell sorting was performed using the FACSAria (Becton Dickinson) or by using magnetic sorting (MACS MicroBead Technology, Miltenyi Biotec) and anti-APC MicroBeads (Miltenyi Biotech) ...
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bioRxiv - Microbiology 2020Quote: ... Mononuclear cells (MNC) were isolated from spleen and lung tissue samples using an OctoMACS tissue dissociation device (Miltenyi Biotec). Lung tissue samples were dissected into approximately 5mm3 pieces and incubated for one hour in a solution of 772.8 U/ml collagenase + 426 U/ml DNase (both from Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... cells were surface-labelled with an APC-conjugated anti-HA antibody (Miltenyi Biotec, Cat# 130-098-404, 1:50) and sorted by flow cytometry ...
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bioRxiv - Developmental Biology 2022Quote: ... Unlabeled cells which passed through the LS column loading CD45 microbeads and LD column (Miltenyi Biotec #130-042-901) retaining weakly labeled cells were defined as the CD45- fraction ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were sorted from pooled spleens of WT C57BL/6 mice by using mouse CD11c MicroBeads (Miltenyi Biotec). Then ...
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bioRxiv - Molecular Biology 2019Quote: Mitochondria were isolated from WT and NSUN2 KO HEK293T cells (1 × 107) using the Mitochondria Isolation Kit (Miltenyi Biotec). Steady-state levels of subunit proteins of mitochondrial respiratory chain complexes were analyzed by immunoblotting with Total OXPHOS Rodent WB Antibody Cocktail (1:250 ...
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bioRxiv - Systems Biology 2019Quote: Cells were washed once with PBS and stained with CD11b-APC or isotype control (Rat IgG2b-APC, Miltenyi Biotec) antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... and TCRβ+CD138+ and TCRβ+CD138− cells were further separated with anti-PE magnetic MicroBeads (Miltenyi Biotec, Auburn, CA). After three washes with PBS ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then collected and processed for intracellular staining with anti-IFN-γ-FITC monoclonal antibody (Miltenyi Biotec, Germany). Changes in the subset of lymphocyte cells and cytokine production (IFN-γ ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) from healthy donors (HD) were used to isolate monocytes (using CD14+ microbeads, Miltenyi Biotec) and to sort CD1c+ DC and slanDCs using FACS (Aria IIIu ...
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bioRxiv - Cancer Biology 2020Quote: ... Positive selection was performed to isolate CD8+ T cells from primary tumors and spleens using CD8a microbeads (Miltenyi Biotec), an LS column ...
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bioRxiv - Cell Biology 2021Quote: T lymphocytes were isolated from healthy donor’s PBMCs via Pan T Cell Isolation Kit (Miltenyi Biotec,130-096-535) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Electroporated cells were seeded on Laminin 521 (Biolamina, Sundbyberg, Sweden) coated plates in StemMACS iPS-Brew XF (Miltenyi Biotec) supplemented with 1x CloneR (Stemcell Technologies ...
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Cell Biology 2021Quote: ... CD11b myeloid cells were purified from PBMCs by positive selection using the anti-CD11b microbeads and columns (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQUANT Analyzer (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... human peripheral blood mononuclear cells were activated with 50□ng/mL OKT3 (Cat. No. 130-093-387, Miltenyi Biotec) and harvested for retroviral transduction on day 2 ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQuant Analyzer (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... cDC2s were further enriched from single cell suspensions by positive isolation with CD11c Microbeads (Miltenyi Biotec; per manufacturer’s instructions) and FACS sorting (MHCII+ CD11c+ CD64- F4/80- CD172a+ XCR1- ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Genomics 2022Quote: ... and processed into single-cell suspensions using the Whole Skin Dissociation Kit (Miltenyi Biotec, Cat No.130-101-540) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2022Quote: CD3+/CD4+ T cells were purified by positive selection using magnetic separation with a CD4+isolation kit (Miltenyi Biotec) using PBMCs from three donors as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Tregs were isolated from peripheral blood mononuclear cells (PBMCs) by negative selection of CD4 (Miltenyi Biotec, Bergisch Gladbach, Germany) followed by positive CD25 selection (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... brain cells were isolated using a 30%/80% Percoll gradient after homogenizing tissue on a gentleMACS dissociator (Miltenyi Biotec). Single cell suspensions were then incubated with purified anti-CD16/32 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: For single-cell sequencing experiments samples were additionally run on a gentleMACS™ Dissociator (Miltenyi Biotec, #130-093-235) with the custom program h_skin_01 (Duration ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were pelleted and resuspended in TMRE buffer and subjected to flow cytometry on a MACSQuant Analyzer (Miltenyi Biotec). For mitochondrial superoxide measurements ...
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bioRxiv - Immunology 2024Quote: ... Spleen qPCR samples were prepared by depletion of CD19+ TCRβ+ cells using biotinylated antibodies and Streptavidin MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the unbound complexes were washed and the cells processed through magnetic columns (Miltenyi Biotec, Catalog No. 130-042-401) following manufacturer instruction ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... CD34+ cells were positively selected through two consecutive runs using immune magnetic beads kits (Miltenyi Biotec, Bergisch-gladbach, Germany) and cryopreserved ...
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bioRxiv - Immunology 2024Quote: ... synchronisation at trophozoite or schizont stage was performed by utilizing magnetic activated cell sorting (Miltenyi Biotec MACS LD columns).
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bioRxiv - Immunology 2024Quote: ... blood and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-APC (130-123-207 ...
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bioRxiv - Cancer Biology 2024Quote: ... Depletion of CD45+ cells in NSCLC samples was performed using CD45 MicroBeads (Miltenyi Biotec, cat. no. 130-045-801) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... The following antibodies were used for cell surface staining: IgA (clone IS11-8E10) (Miltenyi Biotec, cat#: 130-114-002), IgD (clone IA6-2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then pelleted and resuspended in 5 mL PBSF and sorted using a MidiMACS Separator magnet (Miltenyi Biotec) in combination with an LS column (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were enriched as previously described on a CliniMACS Prodigy according to the manufacturer’s instructions (Miltenyi Biotec). Nonhuman primate CD34+ cells were harvested ...
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bioRxiv - Cancer Biology 2022Quote: ... and dead cell removal following the manufacturer’s protocols (Miltenyi Biotec, 130-095-929, 130-104-694, 130-090-101). Suspensions of single cells were processed on a Fluidigm C1 instrument using a C1 Single-Cell mRNA Seq HT IFC and Reagent Kit v2 (Fluidigm ...
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions from tumor tissues were prepared using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: hCD8+ T cells were isolated from commercially available sources (buffy coats from ZenBio Inc. and leukopaks from Miltenyi Biotec) using magnetic separation (positive selection ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were isolated from whole blood by hypotonic lysis of erythrocytes using Red Blood Cell Lysis Solution (Miltenyi Biotec) followed by ice cold PBS/1% BSA/2mM EDTA washes ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor cells were incubated 24 hours in R10 medium (i.e. supplemented or not with 200 ng·mL-1 IFNγ (Miltenyi Biotec)) ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Immunology 2023Quote: ... Further purification of immune cells was done by magnetic enrichment of the CD45+ fraction (130-052-301, Miltenyi Biotec). All cell suspensions were kept in PEB buffer (0.5% BSA and 2 mM EDTA in PBS).