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Citations for Miltenyi Biotec :
2051 - 2100 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... CD4+CD25− Th cells were purified by positive selection with CD4-specific microbeads (Miltenyi Biotec). Memory T-cell subsets were sorted to more than 97% purity as CD4+CD45RA− RO+CD127+CD25− using APC-780-conjugated anti-CD45RA Ab (eBioscience) ...
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bioRxiv - Immunology 2021Quote: ... CD14 cells were purified by anti-CD14 monoclonal antibody (mAb)-conjugated magnetic microbeads (Miltenyi Biotec) and then cultured for 6 days in RPMI 1640 medium (Life Technologies Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... and the BM was depleted of T cells by autoMACS (Miltenyi Biotec, Bergisch Gladbach, Germany) utilizing CD90.2 microbeads (#130-121-278 ...
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bioRxiv - Immunology 2020Quote: ... The percentage of microglia among cells was determined by CD45 (Miltenyi Biotec #130-110-632) and CX3CR1 (Biolegend #149039 ...
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bioRxiv - Immunology 2021Quote: Lung epithelial cells separated by magnetic bead: the anti-mouse CD45 magnetic bead (Miltenyi Biotec) was used to enrich the epithelial cells in single-cell suspension ...
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bioRxiv - Immunology 2020Quote: ... and single-cell suspensions were prepared according to the manufacturer’s instructions (Miltenyi Biotec GmbH, 2008). Cells were blocked with CD16/CD32 Fc block (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: Glial cells and neurons were isolated according to the adult brain dissociation protocol (Miltenyi biotec) form corpus callosum and/or cortex ...
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bioRxiv - Immunology 2022Quote: ... CD14+ cells were isolated from PBMNCs using a magnetic micro-bead CD14+ kit (Miltenyi Biotec), LS columns (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were loaded to a MACSQuant Tyto HS Cartridge (#130-121-549, Miltenyi Biotec) and sorted according to the instrument instructions until 1 x 107 positive events had been collected ...
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bioRxiv - Immunology 2022Quote: ... samples were first positively selected for CD11c+ cells using Mouse UltraPure CD11c Microbeads (Miltenyi Biotec) prior to surface marker staining ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were positively selected with CD4 (L3T4) magnetic microbeads (Miltenyi Biotec, Auburn, CA), pooled and suspended in PBS ...
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bioRxiv - Immunology 2022Quote: ... ECs were purified by magnetic-activated cell sorting (MACS) using anti-CD146 MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... single cells were passed through a 70μm MACS SmartStrainer (Miltenyi Biotec, Cat# 130-098-462) that was pre-wet with 3mL of 20% FBS in PBS into a 15mL conical tube on ice ...
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bioRxiv - Microbiology 2022Quote: ... CD4(+) T cells were purified from PBMCs using CD4 MicroBeads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The cell suspension was first incubated with FcR blocking reagent (Miltenyi Biotec, 130-092-575) for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with cardiac troponin T APC-conjugated antibody (Miltenyi Biotec, 130-120-403) or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... CD4+ T cells were isolated from PBMCs by positive selection using CD4+ microbeads (Miltenyi Biotec) or FACS sorting and subsequently expanded through a REP.
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bioRxiv - Cancer Biology 2022Quote: B cells were isolated from cryopreserved PBMCs by positive selection using CD19+ microbeads (Miltenyi Biotec) and expanded through CD40-CD40L stimulation by culturing cells for 4-5 days with irradiated NIH3T3 feeder cells constitutively expressing CD40L at 37°C in 5% CO2 in B cell medium ...
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bioRxiv - Cancer Biology 2024Quote: ... Cd11b+ (Mac-1+) cells were isolated from unfractionated bone marrow using Cd11b MicroBeads (Miltenyi Biotec) following the manufacturers’ instructions.
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and purified on LS-columns (Miltenyi Biotec) according to the manufacturer’s instructions for positive and negative selections.
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bioRxiv - Cell Biology 2024Quote: ... MEF feeder cells were removed using magnetic feeder removal microbeads (Miltenyi Biotec, 130-095-531) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... cells were re-plated with 10 uM StemMACSTM Y-27632 ROCK inhibitor (ROCKi; Miltenyi Biotec). Media was changed every 24 hours.
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bioRxiv - Molecular Biology 2023Quote: ... CH12-F3 cells were stimulated with agonist anti-CD40 antibody (0.5μg/ml; Miltenyi Biotec; FGK45.5), mouse IL-4 (5 ng/μl ...
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bioRxiv - Genomics 2024Quote: ... we further filtered the cells using a 30μm MACS SmartStrainer (Miltenyi Biotec 130-098-458). Cell counts and viabilities were measured using an automated cell counter (NanoEnTek EVE) ...
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bioRxiv - Cell Biology 2023Quote: ... but with final elution from the MiniMACSTM Large Cell Column (Miltenyi BioTec Ltd, Bisley, UK) into 150μL STM.
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bioRxiv - Immunology 2024Quote: ... for 24hrs and subjected to a Dead Cell Removal kit via magnetic separation (Miltenyi Biotec) and live M- or G-MDSCs were subsequently used for in vitro experiments.
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with either PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec) or S recombinant protein (Sino Biological ...
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bioRxiv - Genomics 2024Quote: ... Edu positive cells were detected using a MACSQuant® Analyzer 10 Flow Cytometer (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells from PBMCs were separated using CD3 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cell medium was replaced with fresh medium supplemented with IFN-γ1b (1ng/ml, Miltenyi Biotec) and TNFα (1ng/ml ...
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bioRxiv - Immunology 2024Quote: Naive T cells from spleens and lymph nodes were purified by MACS microbeads (Miltenyi Biotec) and seeded in RPMI-1640 containing 10% FBS ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... and CD8 T cells were purified using a magnetic bead separation kit (Miltenyi Biotec, Germany). CD8 T cells were cultured in 96 well plates “U” bottom with 5μg/mL of plate-bound anti-CD3 (clone 145-2C11 ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... and CD8 T cells were purified using a magnetic bead separation kit (Miltenyi Biotec, Germany). Three million CD8 T cells were transferred intravenously into RAG-/- mice subsequently infected with L ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the tissue suspension was passed through a 100 µm cell strainer (Miltenyi Biotec, Gaithersburg, MD). Fixed tissue was washed ...
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bioRxiv - Immunology 2023Quote: ... Next we applied the Dead Cell Removal Kit (catalog no. 130-090-101; Miltenyi BioTec) to the remaining cell pellet ...
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bioRxiv - Immunology 2023Quote: ... followed by depletion using magnetic anti-biotin beads (30 µl per 107 cells, Miltenyi Biotec) and the iMag (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... CD4+ T cells were purified using a negative-sorting CD4+ isolation kit (Miltenyi Biotec, Inc.). CD4+ T cell isolation was done following the manufacturer’s instructions and were consistently found to be > 90% pure ...
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bioRxiv - Immunology 2022Quote: ... cells were isolated by positive selection using an LS Column and MidiMACS Separator (Miltenyi Biotec). Cells were resuspended in full DMEM and plated onto collagen I-coated glass or polyacrylamide gels in FluoroDish cell culture dishes (10-mm well ...
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bioRxiv - Genomics 2023Quote: ... we passed the solution through a 30μm cell straining filter (Miltenyi Biotec, Bergisch Gladbach, Germany), which captured the scales (>100 μm length ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with MACS buffer and MACS was performed using an AutoMACS (Miltenyi Biotec) running the program possel_s ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with CD11b Microbeads (1:10, cat # 130-049-601, Miltenyi Biotec) and microglia were isolated using a magnetic MACS separator ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were stained using CD117-APC (Miltenyi Biotec, 130-098-207, 1:50) and CD133-PE (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2023Quote: ... FLs were depleted of hematopoietic cells (Ter119+CD45+Kit+CD71+) using MACS columns (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently activated using the T Cell Activation/Expansion Kit (Miltenyi Biotec, 130-091-441). From this point forward ...
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bioRxiv - Neuroscience 2023Quote: ... The cell nuclei were then resuspended in ice-cold MACS buffer (Miltenyi Biotec, Gaithersburg, MD) for anti-GFP MACS microbeads incubation ...
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bioRxiv - Microbiology 2023Quote: ... Single cell suspensions were obtained by filtering through MACS® SmartStrainers (100 μm, Miltenyi Biotec), centrifuged at 500×g for 10 min (4°C ...
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bioRxiv - Microbiology 2023Quote: ... Single cell suspensions were obtained by filtering through MACS® SmartStrainers (100 μm, Miltenyi Biotec), centrifuged at 300×g for 10 min (4°C ...
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bioRxiv - Immunology 2023Quote: ... The numbers of viable cells were determined using propidium iodide and a MACSQuant (Miltenyi Biotec). Before cell sorting ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Immunology 2023Quote: ... CD45+ cells were isolated by CD45 magnetic beads isolation kit (130-052-301, Miltenyi Biotec).