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Citations for Miltenyi Biotec :
1951 - 2000 of 2221 citations for Rat Oligodendrocyte transcription factor 1 OLIG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic cell dissociation and debris and red blood cell removal were performed using the Adult Brain Dissociation Kit (130-107-677; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: To assess immune cells and endothelia from the lung left lobe of lung was digested using a GentleMACS lung digestion kit (Miltenyi Biotec #130-095-927). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Primary CD4+ T lymphocytes were isolated from peripheral blood mononuclear cells and purified by negative selection using the CD4+ T cell isolation kit (Miltenyi Biotec, 130-096-533). Cells were phenotypically analyzed and activated using anti- CD3/antiCD28 coated plates (Ref BE0001-2 and Ref BE0248 ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of splenocytes from individual mice were prepared and B cell enrichment was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). Plasmablast cell sorting was performed using a FACSAria (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... and later monocytes were enriched from the total bone marrow cells using the Monocyte Isolation Kit (BM) from Miltenyi Biotec (Cat: 130-100-629) through a negative selection process following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... dead cells were removed from the culture via a Miltenyi Biotec Dead Cell Removal Kit and MiniMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Untouched T cells were isolated from PBMCs by negative Magnetic-activated cell sorting (MACS) using the human pan T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T lymphocytes were purified from total PBMC by Naïve CD8+ T Cell Isolation Kit and MACS® separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The ventricles were sectioned into small cubes and processed using mechanical dissociation (gentleMACS) and enzymatic degradation (neonatal heart dissection kit; Miltenyi Biotec, Bergisch Gladbach, Germany). The cell suspension was then filtered and the cells were plated onto glass-bottom dishes (MatTek Corp. ...
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bioRxiv - Cancer Biology 2023Quote: We isolated human umbilical cord blood (hUCB) CD34+ cells from two patients using the Miltenyi CD34 Microbead Kit and MACS separation column (Miltenyi Biotec, Boston, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... immune cells from the colonic lamina propria were extracted using the Lamina Propria Dissociation Kit for mice and gentleMACS dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Cancer Biology 2023Quote: Cell sorting was performed on Control and EIF4G2KD HEC-1A cells following magnetic beads separation of CD133+ and CD133-(CD133 Microbead Kit, Miltenyi Biotec, 130-097-049) and LS-columns (Miltenyi Biotec ...
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bioRxiv - Genomics 2024Quote: ... The cells were centrifuged at 300 x g for 10 minutes and further submitted to live cell sorting with the Magnetic Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101), according to the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... and pDCs were purified using a negative selection MACS beads-based assay (Plasmacytoid Dendritic Cell Isolation Kit II human, Miltenyi Biotec, Bergisch Gladbach, GER), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were sacrificed at predetermined timepoints and human CD45+ cells were isolated from bone marrow and spleen using the mouse cell depletion kit (Miltenyi Biotec, #130-104-694) to achieve >90% purity of human cells.
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bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated into a single cell suspension and the debris were removed using the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) and gentleMACS program 37C_ABDK_02 according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... while the resulting Lin- (lineage-negative) fraction was then enriched for c-Kit expression using CD117 MicroBeads (Miltenyi Biotec, Catalog no: 130-091-224). These cKit-enriched cells were washed ...
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Freshly enriched EVs from WJ-MSC WT and CD81-Snorkel-tag cell supernatants were immunoprecipitated either with hCD81 exosome isolation kit (Miltenyi biotec; 130-110-914) or µMACS™ HA Isolation Kit (Miltenyi biotec ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were transcardially perfused with cold PBS and cortical regions were quickly dissected and enzymatically digested using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) in the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected in free RPMI medium and then dissociated for single-cell suspension by the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells counts were determined by flow cytometer Novocyte (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... The diluted blood was labeled with antibodies directed against Ter119 (FITC, TER-119 1/30 Miltenyi Biotec), CD71 (PE ...
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bioRxiv - Cancer Biology 2019Quote: ... The transfected TI lymphocytes were stimulated with the anti-CD3 antibody (1 μg/mL, OKT3, Miltenyi Biotec) coated on the plate for 84 h ...
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bioRxiv - Cancer Biology 2022Quote: ... After several washing with 1×DPBS containing 0.04% bovine serum albumin (BSA, Miltenyi Biotec, 130-091-376), gastric tumor tissues single cells were acquired with digestive enzymes containing 200 μL Enzyme H ...
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bioRxiv - Immunology 2022Quote: ... 100,000 cells per well were activated with anti-human TransAct (1:100, Miltenyi Biotec 130-111-160) in T cell medium (10 % FCS ...
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bioRxiv - Neuroscience 2021Quote: ... aliquots of cells were stained with: 1) Microglial (Cd11b-APC (M1/70, #130-113-793, Miltenyi Biotec) / Cd45-Vioblue (REA737 ...
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bioRxiv - Cell Biology 2020Quote: ... ESCs culture medium consists of N2B27 medium (Life technology) supplemented with PD0325901 (PD, 1 μM, Miltenyi Biotec), CHIR99021 (CH ...
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bioRxiv - Cell Biology 2021Quote: ... dissociated cells were incubated with CD90 microbeads (Miltenyi Biotec, 130-096-253; 1 μl per 107 cells) to enrich CD90+ NPCs.
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bioRxiv - Developmental Biology 2021Quote: ... and positively selected using 10 uL PDGFRα antibody-coated microbeads (1:10; Miltenyi Biotec #130-101-547). PDGFRα+ stoma was placed into magnetic separation MS columns (Miltenyi Biotec #130-042-021 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with PE/Cy7 anti-human CD44 antibody (Miltenyi Biotec, 130-113-904, 1:50) and CD338-PE (ABCG2-PE ...
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bioRxiv - Immunology 2022Quote: ... Cells were resuspended in PBS containing 1% FBS and incubated with mouse FcR blocking reagent (Miltenyi Biotec) for 10 minutes at 4°C before antibody staining with 1:50 dilution for 30 minutes at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: PBMCs were activated in plates coated with 1 μg/mL anti-CD3 mAb (clone: OKT3, Miltenyi Biotec) and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone ...
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bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE-Vio® 770 anti-human CD14 (clone TÜK4, dilution 1/50, Miltenyi Biotec, Paris, France) antibodies were used for macrophages phenotyping ...
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bioRxiv - Immunology 2023Quote: ... Livers sections were washed again and blocked using 1% Fc Block (MACS, Miltenyi Biotec, Auburn, CA, USA) and 5% BSA in HBSS during 1 hour at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained with mouse anti-human CD19 antibody (Miltenyi Biotec, cat# 130-113-730; 1:50) in the dark for 25 minutes ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Neuroscience 2020Quote: ... brain stem and cerebellum were removed and the remaining cerebrum was freed from meninges and dissociated by enzymatic digestion using the Neural Kit P (Miltenyi Biotec; Cat#130-092-628). Subsequently ...