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Citations for Miltenyi Biotec :
1951 - 2000 of 2056 citations for Mouse Protein DEK DEK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Biotinylated RNA was incubated with constant rotation for 15 minutes at room temperature with μMACs streptavidin beads and was isolated using μMACs columns (μMACs Strepavidin Kit, Miltenyi Biotec 130-074-101) that had been previously equilibrated with wash buffer (100mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533). Per 1 × 107 total cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was obtained by mechanical resuspension followed by filtration through a 40-μm-pore-size cell strainer and dead cel;ls were removed using Dead Cell Removal Kit (Miltenyi Biotec 130-090-101). Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+ ...
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bioRxiv - Cancer Biology 2020Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was enzymatically and mechanically dissociated into a single-cell suspension using the magnetic activated cell sorting (MACS) Neural Tissue Dissociation Kit (Miltenyi Biotec 130-093-231). Myelin was removed using the MACS Myelin Removal Beads (Miltenyi Biotec 130-096-733) ...
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bioRxiv - Pathology 2021Quote: Single cells from hamstring muscles were isolated using a skeletal muscle dissociation kit (Miltenyi Biotech) and GentleMACS Dissociator tissue homogenizer (Miltenyi Biotec, Macquarie Park, Australia) as previously described20 ...
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bioRxiv - Immunology 2020Quote: ... OT-I CD8+ T cells were then purified by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec: 130-104-075). For cell division or microscopy studies ...
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bioRxiv - Cancer Biology 2021Quote: Control and constricted MDA-MB-231 cells were cocultured with human NK cells sorted from peripheral blood using the NK cell isolation Kit (Miltenyi Biotec 130-092-657) at the ratio of 1:20 ...
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bioRxiv - Immunology 2022Quote: ... and used for eosinophil purifications by negative selection on an AutoMACS Pro separator using the Eosinophil Purification Kit (Cat. No. 130-092-010, Miltenyi Biotec, Bergisch Gladbach, Germany). Eosinophil purity was >98% for all samples as determined by counting a minimum of 300 cells on cytospin slides stained with Shandon™ Diff-Kwik™ stains (Cat ...
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bioRxiv - Cancer Biology 2022Quote: B cells were purified by negative selection from single-cell suspensions from spleen using magnetic separation B cell isolation kit (Miltenyi Biotec 130-090-862). Purified cells were cultured for 4 days in RPMI (Corning ...
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bioRxiv - Neuroscience 2021Quote: Mice were perfused with cold PBS and cortical and hippocampal tissues were dissected and dissociated into a single cell suspension using the Adult Brain Dissociation Kit (Miltenyi Biotec 130-107-677), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining cortex from ipsilateral and contralateral sides were dissected and stored in ice-cold Hibernate E (Brain Bits) until being subjected to enzyme digestion to generate single cell suspensions according to the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677). Myelin was removed from the samples using myelin removal beads (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... The whole brain samples were removed and prepared into cell suspension using brain tumor dissociation kit (#130-095-942, Miltenyi Biotec, Bergisch Gladbach, Germany) and gentleMACSTM Dissociator (#130-093-235 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed by Annexin V exposure and PI incorporation using the FITC-Annexin V/PI kit from Miltenyi Biotec (#130-092-052) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... T cells were enriched from spleen homogenate by magnetic-activated cell sorting (MACS) using Miltenyi Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Stefano Rivella) and CD34+ cells were isolated by positive selection using the human CD34 Microbead kit from Miltenyi Biotec (Catalog No.130-046-702) as per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Single cell suspensions were obtained by adapting the protocol described in (Cowan et al., 2020) and using a papain-based dissociation kit (Miltenyi Biotec, 130-092-628). Briefly ...
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions from liver and BM were depleted of lineage-positive (Lin+) cells using a Direct Lineage Cell Depletion Kit (Miltenyi Biotec, 130-110-470) and stained for the following HSPCs ...
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bioRxiv - Immunology 2022Quote: ... MCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, The Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Immunology 2022Quote: Primary NK cells were enriched from normal human peripheral blood mononuclear cells (PBMCs) (Zenbio Inc.) using NK cell isolation kit (Miltenyi Biotec, 130-092-657). The purity of isolated NK cells was confirmed by staining of APC conjugated anti-human CD56 antibody and PE conjugated anti-human CD16 antibody ...
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bioRxiv - Bioengineering 2022Quote: The cell pellet was then incubated in a 4 °C fridge with rocking for 15 minutes in 100 µL of MACS buffer and 25 µL of lineage depletion microbeads from the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Mononuclear cells were isolated by density gradient centrifugation and CD34+ cells were subsequently isolated using the MACS CD34 MicroBead kit and the autoMACS (Miltenyi Biotec, Amsterdam, The Netherlands). CB-MLL-AF9 (here mBlueberry2 ...
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bioRxiv - Cancer Biology 2019Quote: Half of each tumor was dissociated using the Human Tumor Dissociation Kit and Gentle MACS Tissue Dissociator with Heaters (Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s instructions prior to enumeration of live cells using trypan blue.
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bioRxiv - Immunology 2020Quote: ... The supernatant was removed and the pellet resuspended and processed as directed by the Multi-tissue Dissociation Kit I (Miltenyi Biotec, 130-110-201). Brains were enzymatically digested into a single cell suspension by rotating at 37°C for approximately 25 minutes with trituration halfway through incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Gt(ROSA26)SorCreERT2/+ mice as described (Hatsell et al., 2015)for tamoxifen regime using the Skeletal Muscle Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: Brain tissue samples including the cortex and hippocampus obtained from experimental mice that did or did not receive the mCRP treatment were gently dissociated into single-cell suspensions using the Adult Brain Dissociation kit (#130107677, Miltenyi Biotec, Auburn, CA, USA). Single-cell isolation and characterization of CD31+ cells were performed as previously described with a minor modification 10 ...
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bioRxiv - Bioengineering 2021Quote: ... The Lin− fraction of the CB cells and the AML cells were further enriched for CD34 with the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, 130-100-453), which contains microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected into single lobes before being dissociated into single cell suspension using gentleMACS octo-dissociator and Miltenyi lung dissociation kit (Miltenyi Biotec, 130-095-927). Red blood cells (RBCs ...
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bioRxiv - Bioengineering 2021Quote: ... and was then dissociated with an enzymatic and mechanical method using a MACS Neural Tissue Dissociation Kit for postnatal neurons (Miltenyi Biotec, 130-094-802) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... Lung was collected in RPMI and was dissociated into single-cell suspension using the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) as per the company’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Fully de-identified human CD34 cells enriched blood samples were obtained from Bone Marrow and Transplantation Laboratory at UCSF and were MACS sorted using CD34 MicroBead Kit (Miltenyi Biotec, 130-046-702) and incubated with CD3 antibody (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: Purified milk and serum EVs were subjected to multiplex bead-based analysis by flow cytometry using the human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813). EV samples were processed overnight in the MACSPlex Filter Plate as described in the manufacturer’s instructions and in a previously published paper on a systematic evaluation of the assay19 ...
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bioRxiv - Genomics 2022Quote: 3ug of biotinylated RNA and 60pg of in vitro transcribed spike-in RNA (ERCC-00048) in 200ul water was mixed with 100ul of beads from the uMACS Streptavidin kit (Miltenyi Biotec 130-074-101) on a thermoblock at 24°C at 800 RPM for 15 minutes ...
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bioRxiv - Immunology 2022Quote: Splenic B cells from 24-week old MRL/lpr mice were isolated with a Pan B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). B cells were cultured with RPMI-1640 medium (Lonza ...
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bioRxiv - Physiology 2023Quote: Single cardiomyocytes were isolated from the ventricles of C57BL/6J WT mouse neonates aged from postnatal day 0 to 3 by enzymatic and mechanical dissociation in a semi-automated procedure by using the Neonatal Heart Dissociation kit and the GentleMACS™ dissociator (Miltenyi Biotec, Gaithersburg, MD). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Systems Biology 2022Quote: Naïve CD8+ T cells were isolated from the peripheral blood of two healthy donors using the Naive CD8+ T Cell Isolation kit (Miltenyi Biotec, 130-093-244) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The tissue pieces remaining after enzymatic treatment and filtration of the fresh sample were processed using a human tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions to obtain a single-cell suspension ...
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bioRxiv - Cell Biology 2023Quote: MuSCs were isolated from the hindlimb muscles of 11–13-week-old mice following MACS using a Satellite Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and anti-integrin α7 microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... Tregs were isolated from fresh PBMCs by magnetic separation using the CD4+CD25+CD127dim/- human Treg isolation kit (Miltenyi Biotec, Cat: 130-094-775). Tregs were then used directly for analysis or assays.
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bioRxiv - Immunology 2024Quote: ... The remaining submucosal tissue was placed in a conical tube containing fresh RPMI-1640 supplemented with digestion enzymes from the human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) and chopped into small pieces using dissection scissors ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were harvested 48 h post-electroporation and dissected brains were treated with Neural Tissue Dissociation Kit P (Macs Miltenyi Biotec, 130-092-628). Genomic DNA from FAC-sorted RFP+ cells upon in-utero electroporation was obtained by Qiagen QIAmp DNA Minikit (# 157050681 ...
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bioRxiv - Bioengineering 2024Quote: ... The cell pellet was resuspended in 80 µL of FACS buffer and 20 µL of the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268). The cells were incubated for 15 min at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: For primary cardiac myofibroblast enriched cultures cells were first isolated from adult mice hearts using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) and gentleMACS homogenizer (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... The cerebral cortex was cleaned of meninges and the entire cell populations were isolated by enzymatic digestion and mechanical dissociation using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, Bergisch Gladbach, GER) according to manufacturer instructions followed by a 30% density gradient centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic cell dissociation and debris and red blood cell removal were performed using the Adult Brain Dissociation Kit (130-107-677; Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T lymphocytes were purified from total PBMC by Naïve CD8+ T Cell Isolation Kit and MACS® separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...