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Citations for Miltenyi Biotec :
151 - 200 of 1535 citations for Rabbit Anti Human IgG Fc Alexa Fluor 680 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... human and mouse (Miltenyi Biotec). Anti-human CD41 directly conjugated to Alexa Fluor 488 (Clone ...
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bioRxiv - Immunology 2024Quote: ... human FcR Blocking (Miltenyi Biotec), and 5% mouse serum (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... and MACSiBead Particles pre-coated with anti-human CD335 (NKp46) and CD2 antibodies based on manufacturer guidelines (Miltenyi Biotec). Primary adult CD34+ HSCs were cultured at 0.5 x 106 cells per mL in SFEMII medium supplemented with CC110 cytokine cocktail (StemCell).
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec) and used directly for further differentiation into macrophages (Dominguez-Soto et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... with 20% FCS or a whole skin digestion kit from Miltenyi Biotec (Cambridge, U.K.). Single cell suspensions were obtained and subsequently stained with the following antibodies against ...
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bioRxiv - Microbiology 2023Quote: ... Recovered cells were incubated with Fc block (Miltenyi Biotec, Bergusch Gladbach, Germany, 1:100), vortexed ...
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bioRxiv - Immunology 2020Quote: ... and IgG APC IS11-3B2.2.3 (Miltenyi Biotec). Data of labelled cells were acquired using a Beckman Coulter Gallios instrument and FlowJo ™ software (BD ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Immunology 2021Quote: ... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... human” (Miltenyi Biotec, Bergisch Gladbach, Germany) following the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... human (Miltenyi Biotec, Bergisch Gladbach, Germany)].
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bioRxiv - Cell Biology 2022Quote: ... human (Miltenyi Biotec, 130-107-086). 1 μg/ml puromycin (Sigma ...
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bioRxiv - Genomics 2022Quote: ... human (Miltenyi Biotec #130-094-131). Unstimulated naïve CD4+ T cells were cultured in IMDM (Gibco ...
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bioRxiv - Immunology 2019Quote: ... Human FcR-Block (Miltenyi Biotec, Germany) was included to reduce unspecific staining ...
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bioRxiv - Cell Biology 2020Quote: ... human (130-091-379, Miltenyi Biotec), from the negative fraction of the CD14-positive fraction.
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bioRxiv - Cell Biology 2020Quote: ... human (130-050-201, Miltenyi Biotec). The positive fraction was used as CD14-positive monocytes ...
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bioRxiv - Immunology 2022Quote: ... human positive selection kit (Miltenyi Biotec) and total RNA was extracted from hCD45+ cells using NucleoSpin® RNA kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2023Quote: ... human (Miltenyi Biotec, 130-095-929). Removal of debris and red blood cells was performed for tumor resections as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Flt3-ligand (Miltenyi Biotec), recombinant human IL-6 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human IL-6 (Miltenyi Biotec), recombinant human TPO (PeproTech) ...
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bioRxiv - Cancer Biology 2022Quote: ... the human CD19 MicroBeads (Miltenyi Biotec) were used for depletion of CD19+ cells and the CD19 expression of transfected Raji cells were detected using PE-labeled anti-CD19 (Santa Cruz).
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bioRxiv - Immunology 2023Quote: ... human (Miltenyi Biotec 130-096-495) and stimulated with Dynabeads Human T-Activator CD3/CD28 (Miltenyi Biotec 111.31D ...
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bioRxiv - Pathology 2024Quote: ... FcR human blocking reagent (Miltenyi Biotec) and CD14 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... + 5 µL DNase + 0.5% heat-inactivated FCS) with 10 µL FcR blocking reagent (Miltenyi Biotec), 10 µL biotin labeled anti-CD45 antibody (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained for 30 min at 4°C with the following anti-human antibodies: PerCP-Vio700-conjugated REAfinity recombinant anti-CD31 (Miltenyi Biotec, clone REA730, #130-110-673, 1/20), BV421-conjugated anti-Tissue Factor/CD142 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Human T cells were enriched from PBMCs using the EasySep Negative Human T Cell kit (Miltenyi Biotec) according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed with PBS 1X and stained for 30 minutes using CD38 PE (IB60) Mouse Anti-Human (Cat.#130-092-260, Miltenyi Biotec)/CD38 FITC Multiepitope (Cat ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched using human anti-CD8 MicroBeads and the automated magnetic activated cell sorting (autoMACS) system (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor samples and sections were enzymatically and mechanically digested using the human Tumor Dissociation Kit (Miltenyi Biotec) to obtain single-cell suspension ...
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bioRxiv - Cell Biology 2023Quote: ... human CD45+ cells were purified from the remaining fresh murine BM cells using human CD45 MicroBeads (Miltenyi Biotec), and subsequently transplanted into twelve- to thirteen-week-old low-dose busulfan conditioned NBSGW female mice ...
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bioRxiv - Neuroscience 2020Quote: ... cells dissociated as above were immunopurified with mouse monoclonal antibody to Thy-1 (BSJ-1: French and Jeffrey, 1986) using goat anti-mouse IgG conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) (Yamagata et al. ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended and incubated in MACS buffer containing Fc blocker and CD11c MicroBeads (Miltenyi Biotec) according to manufacturer instruction ...
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bioRxiv - Immunology 2021Quote: ... human (Miltenyi Biotec, Bergisch Gladbach, NRW, Germany). Blood CD4+ T cells were purified with a Naïve CD4+ T cell isolation kit II ...
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bioRxiv - Synthetic Biology 2020Quote: ... human (#130-118-342, Miltenyi Biotec, Germany). Samples were subsequently incubated at 25°C for 1 hour with shaking 1000 rpm (Thermo Mixer C ...