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Citations for Miltenyi Biotec :
151 - 200 of 300 citations for CD14 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... monocytes were purified from human PBMC by using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were purified by CD14 selection using MACS magnetic beads (Miltenyi Biotec, Bergisch Glabach, Germany) and cultured in Roswell Park Memorial Institute medium-1640 (RPMI ...
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bioRxiv - Immunology 2022Quote: Monocytes were purified from PBMCs by CD14 positive magnetic selection (Miltenyi Biotec, Bergisch Gladbach, Germany) and plated at 2.106 cells/ml in RPMI 1640 medium containing 10% fetal calf serum (FCS) ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were positively selected from PBMCs with CD14 MicroBeads (Miltenyi Biotec, Cat# 130-050-201). Mo-DCs and macrophages were differentiated from monocytes in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2021Quote: ... human recombinant IL-2 (Miltenyi Biotec) was added to the medium every two days from day 2 on (100 U/ml) ...
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bioRxiv - Immunology 2020Quote: ... or recombinant IFN-α (Miltenyi Biotec), recombinant IL-12 (R&D systems and Peprotech ...
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bioRxiv - Immunology 2020Quote: ... or recombinant IL-15 (Miltenyi Biotec) as described in the text and figure legends ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Flt3-ligand (Miltenyi Biotec), recombinant human IL-6 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human IL-6 (Miltenyi Biotec), recombinant human TPO (PeproTech) ...
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bioRxiv - Microbiology 2020Quote: ... or by magnetic-activated cell sorting (MACS) separation using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) as described previously 46 ...
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bioRxiv - Immunology 2022Quote: ... Pure MOs were then isolated from PBMCs by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotec). Purity was verified by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... Monocytes were isolated from PBMCs with anti-CD14 conjugated magnetic microbeads (130-050-201, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... moDCs were obtained by isolating monocytes from PBMCs using CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), following Miltenyi Biotec’s protocol for generation of moDCs ...
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bioRxiv - Immunology 2021Quote: ... Pure MOs were then isolated from PBMCs by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotec). Purity was verified by flow cytometry ...
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bioRxiv - Immunology 2020Quote: CD14pos cells were isolated from peripheral blood mononuclear cells (PBMC) using CD14 micro-beads (Miltenyi Biotec) with the autoMACS Pro Separator according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Immature human moDCs were generated from PBMCs using CD14 Microbeads (Miltenyi Biotec cat. 130-050-201) and cultured for 6 days with recombinant GM-CSF (R&D Systems cat ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were then purified from freshly isolated PBMCs using a CD14+ selection MACS purification (Miltenyi Biotec) followed by final re-suspension in chemotaxis buffer (Dulbecco’s modified Eagles medium (DMEM ...
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bioRxiv - Immunology 2023Quote: ... CD14+ cells were isolated (>95% pure) using magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). Monocytes were treated with GM-CSF (50 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were positively purified from PBMCs with anti-CD14 magnetic beads (Miltenyi Biotec Inc, Auburn, CA). For in vitro trained immunity experiments ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated with anti-αβTCR Biotin mAb (Miltenyi Biotec) followed by incubation with anti-Biotin mAb microbeads to deplete αβT-cells by magnetic separation using AUTOMACS under depleteS protocol (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Monocytes were isolated from freshly collected PBMCs using CD14 antibody-conjugated microbeads and magnetic columns (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The purity of isolated monocytes was analyzed by flow cytometry using an anti-CD14 antibody (Miltenyi Biotec). The purified monocytes were ready-to-be-used for the Transwell migration and 3D chemotaxis assays as described below.
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bioRxiv - Immunology 2021Quote: ... Monocytes were positively separated from PBMCs with anti-CD14 magnetic microbeads (Miltenyi Biotec Inc., Auburn, CA, USA).
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bioRxiv - Immunology 2020Quote: ... Monocytes were purified by positive sorting using anti-CD14-conjugated magnetic microbeads (Miltenyi Biotec, Bergisch Gladsbach, Germany). The recovered cells were >98% CD14+ as determined by flow cytometry with anti-CD14 Ab (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Physiology 2022Quote: ... The SVF pellet was resuspended in FACS buffer and incubated with anti-CD14 magnetic microbeads (Miltenyi Biotec) and F4/80 to isolate adipose tissue macrophages (ATM) ...
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bioRxiv - Biophysics 2023Quote: ... Human primary classical monocytes (CD14+CD16-) were further isolated using the Classical Monocyte Isolation Kit (Miltenyi Biotec) by magnetic-activated cell sorting (MACS) ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE-Vio® 770 anti-human CD14 (clone TÜK4, dilution 1/50, Miltenyi Biotec, Paris, France) antibodies were used for macrophages phenotyping ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against CD14 (#130-110-583) and HLA-DR (#130-111-943) were obtained from Miltenyi Biotec. Dapi (#422801 ...
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bioRxiv - Immunology 2023Quote: ... Plasmacytoid dendritic cells and monocytes were isolated using anti-BDCA4 or anti-CD14 magnetic microbeads (Miltenyi Biotec) respectively ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant BMP4 was obtained from Miltenyi Biotec and SB431542 was obtained from Tocris Bioscience ...
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bioRxiv - Immunology 2020Quote: ... Mouse anti-human PE anti-CD107a mAb (REA792, from Miltenyi Biotec) was added and cells were cultured at 37°C for one hour ...
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bioRxiv - Immunology 2022Quote: ... Isolated monocytes were analyzed by FACS using an anti CD14+ antibody (catalog 130-113-147, Miltenyi Biotec, Germany) to check their purity ...
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bioRxiv - Immunology 2022Quote: Monocyte isolation was carried out by using Anti-CD14+ Micro Beads (catalog 130-050-201, Miltenyi Biotec, Germany) from PBMCs separated from the blood samples ...
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bioRxiv - Immunology 2019Quote: ... CD14+ alveolar macrophages were purified by positive selection using antibody-coated magnetic beads following manufacturer’s instructions (Miltenyi Biotec). Purity was checked by flow cytometry (Figure S1A ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes were isolated by magnetic labeling with StraightFrom Whole Blood CD14 Microbeads and AutoMacs-directed selection (Miltenyi Biotec). Primary human macrophages were differentiated ex vivo from monocytes by culturing the monocytes in IMDM supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were isolated using the CD14 positive selection method of MACS technology (Miltenyi Biotec, Cat#130-050-201) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Monocyte-derived dendritic cells and monocytes isolated with the CD14 MACS kit from Miltenyi biotec (130-050-201) were collected in a V-bottom plate at 105 cells/well (Thermo scientific # 10462012 ...
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bioRxiv - Immunology 2023Quote: ... according to the manufacturer’s suggestions and monocytes purified using positive selection with paramagnetic CD14 (human) MicroBeads (Miltenyi Biotec). CD14+ monocytes were differentiated into macrophages using 100 ng/mL of recombinant human M-CSF (Immunotools ...
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bioRxiv - Immunology 2024Quote: ... CD14+/CD16+ monocytes were isolated by negative selection magnetic bead column purification (Pan Monocyte Isolation Kit, Miltenyi Biotec). For chromatin accessibility (ATAC-seq) ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked with 0.5μg/ml of anti-CD40 mAb (Miltenyi Biotec) for 15min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... recombinant anti-DC-SIGN (clone REA617, Miltenyi Biotec), mouse anti-CD1a (Novus Biologicals) ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) from healthy donors (HD) were used to isolate monocytes (using CD14+ microbeads, Miltenyi Biotec) and to sort CD1c+ DC and slanDCs using FACS (Aria IIIu ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (Miltenyi Biotec; Cat. No. 130-050-201) and LS Columns (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... purified mAbs were biotin-labelled using One-Step Antibody Biotinylation Kit (Miltenyi Biotec) following the manufacturer’s recommendations and purified using 40K MWCO Desalting Column (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... CD19 (1D3) and CD11b (M1/70) mAbs and anti-biotin microbeads (Miltenyi Biotec), followed by CD4 staining (RM4.5 ...
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bioRxiv - Microbiology 2020Quote: ... purified mAbs were biotin-labeled using One-Step Antibody Biotinylation Kit (Miltenyi Biotec) following manufacturer recommendations and purified using 40K MWCO Desalting Column (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ cells were isolated by MACS using biotinylated anti-CD14+ antibody and anti-biotin microbeads according to the manufacture’s protocol (Miltenyi Biotec). Monocytes were cultured in complete RPMI plus 20 ng/ml recombinant human M-CSF for 7 days to promote differentiation into hMDMs ...