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Citations for Miltenyi Biotec :
151 - 187 of 187 citations for 7 Amino 4 carboxymethylcoumarin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cell suspension was spun down for 5 min at 300 × g and 4°C and processed for MACS using the CD11b/c microbeads according to the manufacturers protocol (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Immunology 2021Quote: ... Cytokines measured from PBMC supernatants were collected after 4 days of incubation with S protein and concentrations were predicted using a standard curve in the LEGENDplex assay (Miltenyi Biotec 741028).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were spun at 400 g for 3 minutes at 4°C and washed in MACS Buffer (Miltenyi Biotec, 130-091-222) supplemented with 4% FBS (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in PBS with 0.5% of Bovine Serum Albumin (BSA, pH 7.2) and incubated for 15 min at 4 °C with myelin removal beads II (Miltenyi Biotec, Cologne, Germany) for myelin and debris removal ...
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bioRxiv - Microbiology 2023Quote: ... Neutrophils were isolated from 4 different donors using the MACSxpress Neutrophil Isolation and MACSxpress Erythrocyte Depletion kits (Miltenyi Biotec Inc., Auburn, CA) and suspended in RPMI 1640 (without phenol red ...
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Immunology 2020Quote: ... and the remaining adherent monocytes were cultured for 48h in the presence of IL-4 (300 U/ml) and GM-CSF (450 U/ml) (both from Miltenyi Biotec, Madrid, Spain) in X-VIVO 15 (BioWhittaker ...
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bioRxiv - Cancer Biology 2022Quote: Samples were processed immediately after surgery and temporarily stored for transport at 4°C in tissue storage solution (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously described ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2, Miltenyi Biotec, 130-117-701) or corresponding IgG1-APC isotype (clone IS5-21F5 ...
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bioRxiv - Bioengineering 2024Quote: ... were obtained after transduction of Jurkat T cells with a serial dilution of viral supernatants and transduction efficiency evaluated after 3-4 days by monitoring cell surface dNGFR expression using an anti-CD271-APC antibody (Miltenyi Biotec., Paris, France) or HLA-A*02 Dextramer-APC (Immudex ...
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bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were adjusted to 5 mg/mL protein concentration and incubated for 1 h at 4 °C with 100 μL of anti-GFP magnetic beads (Miltenyi Biotec, #130-091-125). Bound proteins were analyzed by immunoblotting with anti-GFP (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Immunology 2019Quote: ... centrifuged at 500g for 10 min at 4 °C and resuspended in 0XTD supplemented with 100 ng/ml each of Stem Cell Factor (SCF, Miltenyi Biotec, 130-096-695), Flt3 Ligand (Flt3L ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
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bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec, catalog# 130-042-201).
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bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Nylon wool-enriched splenic T cells from naive or immunized mice were incubated sequentially for 10 min at 4°C with FITC-conjugated anti-mouse γδ TCR or αβ TCR Abs and 15 min at 4°C with anti-FITC Microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained for 30 min at 4°C with the following anti-human antibodies: PerCP-Vio700-conjugated REAfinity recombinant anti-CD31 (Miltenyi Biotec, clone REA730, #130-110-673, 1/20), BV421-conjugated anti-Tissue Factor/CD142 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...