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Citations for Miltenyi Biotec :
151 - 200 of 738 citations for ± trans 1 Boc 4 4 pyridinyl pyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Each cerebral hemisphere was then cut in 4 sagittal slides before being enzymatically and mechanically digested using a gentleMACSTM Dissociator (#130-093-235, Miltenyi Biotec, 37C_ABDK_01 program) and the Adult Brain Dissociation kit (#130-107-677 ...
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bioRxiv - Immunology 2020Quote: ... and anti-TIM-3 VioBright FITC (clone REA602, Miltenyi Biotec, Cat. 130-109-711, 1:50). Cells were washed in PBS and analysed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
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bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
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bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Developmental Biology 2024Quote: ... centrifuged (200 g for 3 min at 4°C) and resuspended in a solution composed by 80% FACS buffer and 20% anti-rat IgG MicroBeads (Miltenyi Biotec, 130-048-501). Cells were incubated in the mixture for 15 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Immunology 2022Quote: ... Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec, catalog# 130-042-201).
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bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 μM GSK3i (Miltenyi Biotec) and 10 μM of ROCKi (Y-27632 ...
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bioRxiv - Cancer Biology 2024Quote: ... stained cells were washed 3 times with MACS Buffer (1:20 in PBS, Miltenyi Biotec, 130-091-376) to remove any remaining unbound antibody ...
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Nylon wool-enriched splenic T cells from naive or immunized mice were incubated sequentially for 10 min at 4°C with FITC-conjugated anti-mouse γδ TCR or αβ TCR Abs and 15 min at 4°C with anti-FITC Microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
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bioRxiv - Developmental Biology 2020Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec), together abbreviated as 2i ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were added to the medium ...
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bioRxiv - Developmental Biology 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec). Cells were maintained at 37°C with 6% CO2.
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bioRxiv - Cell Biology 2020Quote: ... CHIR99021 (CH, 3 μM, Miltenyi Biotec) and leukemia inhibitory factor (LIF ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM CHIR 99021 (Miltenyi Biotec) and 150 μM LAAP (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were also added to the medium ...
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bioRxiv - Molecular Biology 2024Quote: ... MDSL and TF-1 were additionally supplemented with 25ng/mL of recombinant human IL-3 (Miltenyi Biotec, 130-095-069), and MDS-L with 50nM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 25 ng/ml IL-3 (Miltenyi Biotec), 2 mM Glutamax (Invitrogen Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... 25 ng/ml IL-3 (Miltenyi Biotec), 100 ng/ml M-CSF (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec). Cultures were maintained in HPLM media with 5% dialyzed FBS (Sigma Aldrich ...
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Genomics 2020Quote: ... and passed through 3 LS Columns (Miltenyi Biotec). Mouse embryonic stem cells (ESC ...
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bioRxiv - Developmental Biology 2022Quote: ... using the Multi Tissue Dissociation Kit 3 (Miltenyi Biotec), and pooled ...
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bioRxiv - Cell Biology 2024Quote: ... 3 μM of StemMACS™ CHIR99021 (CHIR; Miltenyi Biotec) were also added ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 ng/ml anti-CD3 antibody (OKT-3) (Miltenyi Biotec) and 3000 IU/ml hrIL-2 ...
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bioRxiv - Cell Biology 2022Quote: ... and dissociated with MACS MultiTissue Dissociation Kit 3 (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 3) Astrocytic (ACSA2-APC (REA969, #130-116-245, Miltenyi Biotec)) ...
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bioRxiv - Immunology 2024Quote: ... 30 ng/ml anti-CD3 antibody (OKT-3) (Miltenyi Biotec) and 3000 IU/ml hrIL-2 as previously reported12 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... liver and brain samples were homogenised in PBS (1-3 mL, adjusted to tissue weight) using gentleMACS™ M tubes (Miltenyi Biotec Ltd, Bisley, Surrey) and gentleMACS™ octo dissociator using the standard protein program ...
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bioRxiv - Cell Biology 2021Quote: ... and FLT3-L (Fms-related tyrosine kinase 3 ligand; Miltenyi Biotec). Cells were cultured at 37°C ...
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bioRxiv - Genomics 2023Quote: ... 10 µg/ml NT-3 (Miltenyi Biotec, cat# 130-093-973), and 1 µg/ml laminin (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/ml NT-3 (Miltenyi Biotec, cat# 130-093-973), 100 µM β-mercaptoethanol and 2 µg/ml Dox ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 ng/mL NT-3 (Miltenyi Biotec, #130-093-973) from day 34 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3 ng/ml IL-15 (#130-093-955, Miltenyi Biotec) for T cells ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3 ng/ml IL-15 (#130-093-955, Miltenyi Biotec).
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bioRxiv - Physiology 2024Quote: ... cardiomyocytes were dissociated using the Multi-dissociation kit 3 (Miltenyi Biotec) and sorted using the Pluripotent Stem-Cell (PSC)-derived Cardiomyocyte Isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... The library was selected for 3 rounds with streptavidin beads (Miltenyi Biotec) loaded with biotinylated TCR and then for 1-2 additional rounds with tetramers of TCR and anti-AlexaFluor647 microbeads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... (3) Chondrogenic medium: StemMACSTM ChondroDiff Medium from Miltenyi Biotec (Bergisch Gladbach, Germany). MSCs were cultured in 12-well plates ...
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bioRxiv - Immunology 2024Quote: ... freshly harvested tissues were incubated with appropriate enzymes (Miltenyi Biotec, Table 3) followed by mechanical dissociation (gentleMACS ...