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Citations for Miltenyi Biotec :
1851 - 1900 of 2241 citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The CD4+ T cells were then enriched by negative selection using a CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were isolated by magnetic cell separation with a NK cell isolation kit and an AutoMACS Pro (Miltenyi Biotec, San Jose, CA). NK cells were expanded and activated for 14 days using mouse 10 ng/ml IL-15/IL-15Rα conjugate ...
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bioRxiv - Microbiology 2024Quote: ... human monocytes were isolated by negative selection with magnetic microbeads according to the manufacturer’s instructions (Pan Monocyte Isolation Kit) (Miltenyi Biotec, Bergisch Gladbach, Germany). A total of 3 x 106 monocytes/mL were seeded in complete RPMI medium in hydrophobic Lumox dishes (Sarstedt ...
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bioRxiv - Immunology 2024Quote: ... to obtain monocyte-derived macrophages which were purified with magnetic bead separation (CD14 negative isolation protocol, Monocyte Isolation Kit II. Miltenyi Biotec, Auburn, CA). Isolated CD14+ monocyte-derived macrophages (MDMs ...
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bioRxiv - Immunology 2024Quote: ... from a cell suspension and harvested from the crushed spleens of OT-I mice using a CD8a+ T-Cell Isolation Kit and magnetic-associated cell sorting (MACS) according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Similarly ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... we further isolated the CD34+ hematopoietic stem cells (HSCs) using magnetic-activated cell sorting according to the manufacturer’s instructions (CD34 MicroBead Kit, Miltenyi Biotec #130-046-702). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... and processed into a single cell suspension according to the manufacturers’ protocol using the adult brain dissociation kit (Miltenyi Biotec 130-107-677). Cells were Fc blocked (Biolegend #101320 ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... the negative fraction of CD8+ T cells selection was collected and incubated with the Tumor Cell Isolation Kit (Miltenyi Biotec, #130-108-339) for 15 min incubation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: CD3+ T cells were enriched from the BM of 30 MM patients using the Pan T cell isolation kit with MS columns (both Miltenyi Biotec, Bergisch, Germany). 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector to target ratio ranging from 7.75:1 to 9:1 ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary NK cells were isolated from the spleens of mice (8 weeks old) by using the NK cell isolation kits according to the manufacturer’s instructions (Miltenyi Biotec #130-090-864). Primary NK cells were cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2023Quote: T2 and T3 cells were then depleted for CD45+ cells using the Miltenyi LD column prep kit (Miltenyi Biotec Inc., Charlestown, MA, USA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Plasmacytoid dendritic cells were isolated by negative selection from prepared PBMCs with the Human Plasmacytoid Dendritic Cell Isolation Kit-II from Miltenyi Biotec (Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were isolated from the spleens of 4T1 tumor-bearing mice using a Mouse MDSC Isolation Kit (Miltenyi Biotec, Cat# 130-094-538) and plated in RPMI1640 supplemented with 10% FBS and antibiotics ...
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bioRxiv - Immunology 2022Quote: B cells from PBMCs of uninfected controls or of COVID-19 convalescent individuals were enriched using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Cancer Biology 2023Quote: Separations were performed on Control and EIF4G2KD HEC-1A cells using magnetic beads for CD133 (CD133 Microbead Kit, Miltenyi Biotec, 130-097-049), or CD44 (CD44 Microbeads Kit ...
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bioRxiv - Cancer Biology 2024Quote: Mouse cells were depleted from the xenograft tumor to enrich for human cells using Mouse Cell Depletion Kit (Miltenyi Biotec, 130-104-694) as previously described24 ...
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bioRxiv - Cancer Biology 2024Quote: Primary human monocytes were isolated from PBMCs from three donors using a negative selection classical monocyte isolation kit (Miltenyi Biotec 130-117-337) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD34+ HPCs were isolated using a CD34 MicroBead kit according to manufacturer’s instructions (magnetically activated cell sorting or MACS; Miltenyi Biotec, San Diego, CA). Pure populations of CD34+ HPCs were infected with TB40/E-WT or TB40/E-ΔUL138STOP HCMV (MOI = 2 ...
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bioRxiv - Immunology 2024Quote: ... Primary monocytes and neutrophils were isolated from mouse bone marrow using negative isolation kits (Miltenyi Biotec, 130-100-629 and 130-097-658). Cells were cultured in RPMI 1640 media (monocytes ...
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bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was incubated for 1 h on ice with anti-GFP magnetic beads (Miltenyi Biotec), and beads were washed 4 times with extraction buffer on the magnetic field ...
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bioRxiv - Neuroscience 2020Quote: ... ~100 μg Fe / 1 × 106 cells (40 μl Miltenyi Biotec FeraTrack SPIO / 2 ml culture medium) was chosen according to cell viability and manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining (1 ×106 PBMC) was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin (v/v) and 50 ng/ml M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) and seeded at a density of 5 x 105 cells per well of a 6-well plate ...
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bioRxiv - Cell Biology 2019Quote: Reprogramming intermediates were labelled with Anti-SSEA-1 (CD15) MicroBeads (Cat.#130-094-530, Miltenyi Biotec) and magnetically isolated according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The cell suspension was then labeled for oligodendrocyte marker 1:50 anti-O4-PE (Miltenyi Biotec), endhothelial cell marker 1:50 anti-CD31-PE (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were stained with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 min on ice ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The pellet was resuspended in 100μl of 1:10 dilution of FcR blocking reagent (Miltenyi Biotec) in DPBS and incubated at 4°C for 10 minutes in sterile FACS tubes ...
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bioRxiv - Physiology 2019Quote: ... 1×105 cells were stained in triplicates to evaluate SLAMF6 expression using anti-SLAMF6 (Miltenyi Biotec) or the corresponding isotype control ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with Anti-FITC MicroBeads (Miltenyi Biotec, Cat. No. 130-048-701, 1:10) for 15 min and separated on a magnetic column (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended in 1 mL 0.5% BSA in D-PBS (#130-091-376, Miltenyi Biotec) and filtered through a 35 µm filter (#352235 ...
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bioRxiv - Cell Biology 2021Quote: ... washed once using PBS+BSA (1%) solution and resuspended with MACS MicroBeads (Miltenyi Biotec, Waltham, MA). Dead cells were removed using MS Columns (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... Gonadal cells suspension was incubated with Anti-SSEA-1 (CD15) Microbeads (Miltenyi Biotec, 130-094-530). After washing ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 1 ml FACS buffer (autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) and used for cell counting ...
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bioRxiv - Immunology 2020Quote: ... and anti-TIM-3 VioBright FITC (clone REA602, Miltenyi Biotec, Cat. 130-109-711, 1:50). Cells were washed in PBS and analysed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 1 ml FACS buffer (autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) and used for cell counting.
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bioRxiv - Molecular Biology 2022Quote: ... cells were resuspended in 1% PBA before being analyzed in the MACSQuant Analyzer 10 (Miltenyi Biotec). FITC signal (LGR5+ cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... and APC-Vio770-conjugated anti-CD11b/c (1:100, Miltenyi Biotec Cat# 130-121- 054, RRID:AB_2752215) for15 minutes at 4°C in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... CAR-T cells were stained using anti-CD90.1-VioBlue (Miltenyi Biotec, 130-112-878, 1:50), anti-c-myc-biotin and anti-biotin-PE (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... OT-1 or Thy1.1 OT1 cells were purified via CD8a (Ly-2) MACS microbeads (Miltenyi Biotec), unless otherwise indicated ...
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bioRxiv - Cancer Biology 2024Quote: ... a minimum of 1×104 events were collected per sample on a MACSQuant Analyzer (Miltenyi Biotec) and analyzed using FlowJo v.10 (Becton ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue ...
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bioRxiv - Cancer Biology 2023Quote: ... packed HD cells were diluted 1:10 with autoMACS® Running Buffer (MACS buffer, Miltenyi Biotec) at 4°C and distributed at 5 mL per LS column (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 1 mL 0.5% BSA in D-PBS (#130-091-376, Miltenyi Biotec) and filtered through a 35 µm filter (#352235 ...