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Citations for Miltenyi Biotec :
1751 - 1800 of 1811 citations for Rat Aflatoxin B1 Aldehyde Reductase Member 3 AKR7A3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
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bioRxiv - Immunology 2023Quote: To assess immune cells and endothelia from the lung left lobe of lung was digested using a GentleMACS lung digestion kit (Miltenyi Biotec #130-095-927). In brief ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of splenocytes from individual mice were prepared and B cell enrichment was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). Plasmablast cell sorting was performed using a FACSAria (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... and later monocytes were enriched from the total bone marrow cells using the Monocyte Isolation Kit (BM) from Miltenyi Biotec (Cat: 130-100-629) through a negative selection process following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... dead cells were removed from the culture via a Miltenyi Biotec Dead Cell Removal Kit and MiniMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Untouched T cells were isolated from PBMCs by negative Magnetic-activated cell sorting (MACS) using the human pan T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The ventricles were sectioned into small cubes and processed using mechanical dissociation (gentleMACS) and enzymatic degradation (neonatal heart dissection kit; Miltenyi Biotec, Bergisch Gladbach, Germany). The cell suspension was then filtered and the cells were plated onto glass-bottom dishes (MatTek Corp. ...
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bioRxiv - Microbiology 2023Quote: ... immune cells from the colonic lamina propria were extracted using the Lamina Propria Dissociation Kit for mice and gentleMACS dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Cancer Biology 2023Quote: We isolated human umbilical cord blood (hUCB) CD34+ cells from two patients using the Miltenyi CD34 Microbead Kit and MACS separation column (Miltenyi Biotec, Boston, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cell sorting was performed on Control and EIF4G2KD HEC-1A cells following magnetic beads separation of CD133+ and CD133-(CD133 Microbead Kit, Miltenyi Biotec, 130-097-049) and LS-columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... isolated mononuclear cells were positively selected for CD34 using the CD34+ Microbead kit Ultrapure (Miltenyi Biotec, San Diego, CA, USA, cat.: 130-100-453). Cells were cultured at 1.5×105–2.5×105 cells/mL in CellGenix® GMP Stem Cell Growth Medium (SCGM ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Cancer Biology 2024Quote: ... while the resulting Lin- (lineage-negative) fraction was then enriched for c-Kit expression using CD117 MicroBeads (Miltenyi Biotec, Catalog no: 130-091-224). These cKit-enriched cells were washed ...
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bioRxiv - Immunology 2024Quote: ... and pDCs were purified using a negative selection MACS beads-based assay (Plasmacytoid Dendritic Cell Isolation Kit II human, Miltenyi Biotec, Bergisch Gladbach, GER), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were sacrificed at predetermined timepoints and human CD45+ cells were isolated from bone marrow and spleen using the mouse cell depletion kit (Miltenyi Biotec, #130-104-694) to achieve >90% purity of human cells.
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bioRxiv - Genomics 2024Quote: ... The cells were centrifuged at 300 x g for 10 minutes and further submitted to live cell sorting with the Magnetic Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101), according to the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was dissociated into a single cell suspension and the debris were removed using the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) and gentleMACS program 37C_ABDK_02 according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were transcardially perfused with cold PBS and cortical regions were quickly dissected and enzymatically digested using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) in the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: Freshly enriched EVs from WJ-MSC WT and CD81-Snorkel-tag cell supernatants were immunoprecipitated either with hCD81 exosome isolation kit (Miltenyi biotec; 130-110-914) or µMACS™ HA Isolation Kit (Miltenyi biotec ...
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bioRxiv - Immunology 2024Quote: ... The lungs were collected in free RPMI medium and then dissociated for single-cell suspension by the Miltenyi Lung Digest Kit and Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells counts were determined by flow cytometer Novocyte (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Neuroscience 2020Quote: ... brain stem and cerebellum were removed and the remaining cerebrum was freed from meninges and dissociated by enzymatic digestion using the Neural Kit P (Miltenyi Biotec; Cat#130-092-628). Subsequently ...
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bioRxiv - Immunology 2019Quote: ... CD8 T cells were isolated from single-cell splenocyte suspensions using the Miltenyi CD8a+ T cell isolation kit (Miltenyi Biotec, Inc. Cat.130-104-075) according the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... supernatants were collected and stored at −80 °C until analysis for cytokine secretion using the human MACSplex cytokine 12-kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... We magnetically isolated all CD4+ T cells from each sample using a human CD4+ T cell negative isolation kit (Miltenyi biotec, Cat #130-096-533) per manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were enriched by positive selection for c-Kit expression using anti-CD117 immunomagnetic beads (130-091-224, Miltenyi Biotec Inc. 1:5 dilution). Second ...
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bioRxiv - Immunology 2021Quote: Intestinal lymphocytes were isolated from RAG1-/- recipients using a lamina propria dissociation kit according to the manufacturer’s instructions (Miltenyi Biotec, Cat. No.: 130-097-410). Briefly ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow was flushed from femurs and tibias and HSCs were isolated by negative selection with the Direct Lineage Depletion Kit (Miltenyi Biotec, Cat#130-110-470) using MACS technology ...
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bioRxiv - Developmental Biology 2022Quote: ... The right ventricle was perfused with ice cold PBS and the lungs were harvested quickly and homogenized and dissociated using the Miltenyi MACS lung dissociation kit (Miltenyi Biotec, cat # 130-095-927), using the GentleMacs program m-Lung-1 on the AUTO MaCs ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi tissues were dissociated to single-cell suspension by enzymatic degradation using a MACS Technology neural tissue dissociation kit (Miltenyi Biotec, Cat# 130-092-628) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Spleens from four- to six-week-old mice were processed to single cell suspensions and CD4+ T cells were enriched using mouse CD4 (L3T4) Microbeads kit (Miltenyi Biotec Cat # 130-117-043) according to manufacturer instructions ...
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bioRxiv - Pathology 2023Quote: Splenic CD4 T cells from WT mice were enriched using naive untouched CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec, cat. No. 130-104-453). Isolated CD4 T cells (1 x106 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cut along the coronal plane with a razor into three pieces and digested into cell suspensions using a Multi Tissue Dissociation kit 1 (Miltenyi Biotec, Germany; #130-110-201) and a gentleMACS™ OctoDissociator with heaters (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2023Quote: ... The right ventricle was perfused with ice cold PBS and the lungs were harvested quickly and homogenized and dissociated using the Miltenyi MACS lung dissociation kit (Miltenyi Biotec, cat # 130-095-927), using the GentleMacs program m-Lung-1 on the AUTO MaCs ...
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bioRxiv - Cell Biology 2023Quote: Murine nCMs were isolated from the ventricles of 1-2 days old wildtype C57BL/6 and Lmnaflox/flox mouse pups using MACS neonatal heart dissociation kit according to the manufacturer (Miltenyi Biotec cat# 130-098-373). Following digestion ...
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bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
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bioRxiv - Cancer Biology 2023Quote: ... the pellet was incubated with RBC lysis buffer for 5 min on ice to remove red blood cells (RBC) and then used for Treg isolation with a CD4+CD25+ Treg isolation kit (Miltenyi Biotec, cat # 130-091-041), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Biophysics 2024Quote: Primary human NK cells from healthy donors were isolated from blood samples provided by the EFS (Marseille, France) by negative selection using the MACSxpress Whole Blood human NK cell isolation kit (Miltenyi Biotec cat. 130-098-185), according to the manufacturer protocol ...