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Citations for Miltenyi Biotec :
1701 - 1750 of 1905 citations for Rat Hepatitis A virus cellular receptor 2 homolog HAVCR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Epithelial cells were isolated from colon samples in the pre-digestion buffer using the Lamina Propria Dissociation Kit (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... A purified monocyte fraction was isolated from the thawed cells using the negative selection MACS® Pan Monocyte Isolation Kit (Miltenyi Biotec) with LS columns according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... liver tissues were cut into small pieces and dissociated on the gentleMACS Dissociator with the Mouse Liver Dissociation Kit (Miltenyi Biotec, 130105807). Leukocytes were enriched by centrifugation with 40% Percoll ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic-activated cell sorting (MACS) with the CD34 Microbead Kit (catalog number #130-046-702, Miltenyi Biotec, Germany) and further purified by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Developmental Biology 2023Quote: ... single-cell suspensions were generated by dissociation of the organoids with a papain-based neural dissociation kit (Miltenyi Biotec, 130-092-628). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T cells were magnetically separated by negative selection with a Human Pan T cell isolation Kit (#130-096-535, Miltenyi Biotec, Germany) and stored long-term in Bambanker serum-free cell freezing medium (#BB01 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Lamina propria lymphocytes were isolated using a lamina propria dissociation kit in combination with a gentleMACS tissue dissociator according to the manufacturer’s instructions (Miltenyi Biotec, Gaithersburg, MD). In brief ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from RBC-depleted splenocytes using the Miltenyi CD8+ negative selection kit (Miltenyi Biotec, Cat # 130-104-075). Reporter-expressing CD8+ T cells were sorted using a BD Aria III ...
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bioRxiv - Immunology 2023Quote: ... naïve 8.3 CD8 T cells from the LNs of non-diabetic NY8.3 NOD mice were purified using two rounds of the naïve CD8 T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
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bioRxiv - Cancer Biology 2023Quote: ... RBCs and debris of dead cells in single cell suspensions were respectively removed using Red Blood Cell Lysis Solution (10x) Kit (Miltenyi Biotec, Germany) and Dead Cell Removal Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Cell Biology 2023Quote: ... we purified some of the myoblast cultures using the mouse Satellite Cell Isolation Kit (130-104-268, Miltenyi Biotec, Bergisch-Gladbach, Germany) when necessary due to higher fractions of fibroblasts (e.g. ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were dissected from the limb and dissociated by shaking for 45 min at 37°C in an enzyme mix from the tumor dissociation kit (Miltenyi Biotec, 130096730). Cell suspension was then strained through a 40 µm filter ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Immunology 2023Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...
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bioRxiv - Microbiology 2023Quote: ... Neutrophils were isolated from 4 different donors using the MACSxpress Neutrophil Isolation and MACSxpress Erythrocyte Depletion kits (Miltenyi Biotec Inc., Auburn, CA) and suspended in RPMI 1640 (without phenol red ...
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bioRxiv - Physiology 2023Quote: ... CD4+ T cells were then purified from the isolated PBMCs by negative selection using humanCD4+ T cell isolation kits (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Mouse CD4+ T cells were purified by negative selection using a CD4+ T cell isolation kit (Miltenyi Biotec, cat. # 130-104-454). For CD8 depletion ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brains from Control and Serpina3n cKO mice were processed using the Neural Tissue Dissociation Kit (P) (Cat# 130-092-628, Miltenyi Biotec, Germany) in conjunction with the gentleMACS Dissociator (Cat# 130-093-235 ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from Day15 B16.F10 melanomas of C57BL/6 mice or α-SMAcreAtg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). 5 x 104 cells CAFs were seeded on poly-L-Lysine coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: ... melanomas of α-SMAcreAtg5fl/fl and Atg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). RNA was extracted with the Macherey-Nagel NucleoSpin® RNA kit ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: Neonatal mouse cardiac myocytes (NMCMs) were isolated using the MACS cell separation kit (Miltenyi Biotec: Catalog #130-100-825 and #130-098-373). Briefly ...
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bioRxiv - Immunology 2021Quote: ... were isolated by gradient centrifugation and then enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638).
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Immunology 2021Quote: B cells from spleens and lymph node of naïve mice were purified by magnetic separation using a pan-B cell isolation kit (Miltenyi Biotec #130-095-813), then incubated overnight with 30 μg/ml LPS (Sigma #L2630 ...
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bioRxiv - Neuroscience 2021Quote: ... using the Adult Brain Dissociation Kit (Miltenyi, 130-107-677) on program 37C_ABDK_01 of the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427) allowing for simultaneous enzymatic and mechanical tissue disruption ...
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bioRxiv - Neuroscience 2021Quote: ... meninges removed and brains of two mice pooled in a C-tube for tissue dissociation with the Neural Tissue Dissociation Kit (Miltenyi, 130-092-628) on program 37C_NTDK_1 of the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427) allowing for simultaneous enzymatic and mechanical tissue disruption ...
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bioRxiv - Immunology 2019Quote: ... Cells were counted and CD8 T cells isolated using a magnetic activated cell sorting negative selection (MACS) kit according to the manufacturer’s instructions (Miltenyi Biotec, #130-104-075). 96 well plates were coated over night at 4°C with 1µg/mL anti CD3e (BD ...
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bioRxiv - Neuroscience 2019Quote: ... The lysate was cleared by centrifugation incubated with anti-GFP/myc antibody coated magnetic beads (MultiMACS GFP Isolation Kit #130-094-253 or Miltenyi Biotec GmbH, Germany). The co-immunoprecipitation was done following the manufacturer’s protocol (Mitenyibiotec ...
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bioRxiv - Cancer Biology 2021Quote: ... Freshly resected tumor tissue was dissociated into single cell suspensions using a tumor dissociation kit (130-096-730, Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... were isolated from the spleen of naive Rdh10f/f Cd4Cre Rag1-/- OT-I CD45.1+ CD45.2+ (Rdh10CKO) or Rdh10f/f Rag1-/- OT-I CD45.1+ (control) mice using the Pan T Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany). For the adoptive transfer of memory T cells ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant was removed and dead cells were removed using the Dead Cells Removal Kit according to the manufacturer’s instruction (Miltenyi Biotec, 130-090-101). The collected effluent was spun (5 min ...
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bioRxiv - Microbiology 2019Quote: ... cells were either sub-cultured (Uns-C) or supplemented with kynurenic acid buffer and sorted according the manufacturer’s instructions using the Microbead Kit (Miltenyi Biotec #130-095-826). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... The resected tumour samples were processed to a single-cell suspension using the commercial Tumour Dissociation Kit (30095929, Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... MCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Neuroscience 2022Quote: Dissociation of cortex and corpus callosum from mice brain was done using neural tissue dissociation kit (P) (Miltenyi Biotec; ref 130–093-231). Briefly ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... T cells were magnetically separated from other splenocytes using the Mouse Pan-T Cell Isolation Kit II (Miltenyi Biotec, Cat# 130-095-130), labeled with CellTrace™ CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The DG was microdissected and the tissue was enzymatically dissociated using the Neural Tissue Dissociation Kit (Miltenyi Biotec, Germany, cat# 130-092-628) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Mobilized PBMCs or cord blood were enriched for CD34+ cells using magnetic beads according to manufacturer’s protocol (CD34 MicroBead Kit, Miltenyi Biotec, Bergisch Gladbach, Germany). CD3 depletion and CD34 enrichment were validated by flow cytometry unless specified otherwise ...
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bioRxiv - Cancer Biology 2022Quote: ... excised tumors (200 mg) were dissociated in RPMI media containing enzyme cocktail mix from the mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA) and placed into gentleMACS dissociators per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Dead cell removal was performed if the viability was lower than 80% using Dead Cell Removal Kit (Miltenyi Biotec, Cat#130-090-101) as per manufacturer’s recommendations.
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bioRxiv - Immunology 2019Quote: Peripheral blood neutrophils from human blood were purified using the Miltenyi Biotech MACSxpress Neutrophil Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, #130-104-434) and residual red blood cells were lysed using MACSxpress Erythrocyte Depletion Kit (Miltenyi Biotec ...