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Citations for Miltenyi Biotec :
1651 - 1700 of 3316 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... slides were incubated for 1 h at room temperature in the dark with a cardiac troponin-T antibody conjugated to APC (130-120-543; Miltenyi Biotec) prepared at a 1:100 dilution in PBS ...
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bioRxiv - Plant Biology 2022Quote: ... The blots were then incubated at room temperature with the monoclonal α-GFP antibody (1:5000) (Miltenyi Biotec, 130-091-833), α-RFP antibody (1:5000 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA) and stained with 1 μl of an APC-conjugated CD21 specific antibody (clone: HB5; 130-101-739, Miltenyi Biotec). The cells were incubated at 4 °C in the dark for 20 min and washed with and resuspended in 200 μl FACS staining buffer ...
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bioRxiv - Immunology 2020Quote: ... For CECs or mock depletion samples were stained using anti-CD71 or isotype control biotin-conjugated antibody and fractioned using streptavidin linked magnetic beads (Miltenyi Biotec) according to our previous reports 24–26,31,34 ...
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bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs) for either the 8-color MACSQuant Analyzer (Miltenyi Biotec), or the 14-color LSRFortessa™ X-20 flow-cytometers (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
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bioRxiv - Immunology 2024Quote: CAR+ T-cells were purified using a biotin-conjugated AffiniPure Goat Anti-Mouse IgG F(ab’)₂ fragment specific antibody and anti-biotin microbeads (Miltenyi Biotec). In the case of dual knock-in TET2-TRAC CAR T-cells ...
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bioRxiv - Microbiology 2024Quote: ... Samples were divided into equal parts (250 μL each) and incubated with 50 μL of magnetic microparticles coated with monoclonal HA or MYC antibodies (MACS purification system, Miltenyi Biotec) at 4°C for 1 hour under rotation (10 rpm) ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25- Tcon were isolated from the CD25- fraction obtained from the same samples by negative selection using FITC-conjugated antibodies and anti-FITC Microbeads (Miltenyi Biotec) as described above (T cell isolation) ...
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bioRxiv - Immunology 2023Quote: ... at a final concentration of 1 µg/mL in the presence of a blocking CD40 antibody (1 µg/mL; Miltenyi Biotec) and CD28 antibody (1 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Magnetic enrichment was carried out on an AutoMACS using microbeads conjugated to antibodies recognizing biotin or mouse CD117 (all from Miltenyi Biotec). HSCs (CD45.2+ ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were enriched by MACS using an in-house biotinylated anti-EGFR antibody (Cetuximab, Eli Lilly and Company, Indianapolis, IN, USA) and anti-Biotin Microbeads (Miltenyi Biotec). Purity of enriched CAR modified T cells was analyzed as described above ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Negative controls were incubated with fluorochrome-labeled irrelevant isotype control antibody (REA Control APC, mouse cod. 130-113-446 Miltenyi Biotec).
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bioRxiv - Cell Biology 2024Quote: ... 100 μL of each sample was removed and set aside as representative “crude mitochondrial fraction.” The remaining 300 μL was diluted to 2 mL total with ICB and combined with 60 μL of superparamagnetic microbeads coated with mouse IgG1 antibodies against GFP (#130-091-125; Miltenyi Biotec). These samples were then placed on a rotating wheel at +4°C for 1 hr ...
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bioRxiv - Plant Biology 2024Quote: ... Protein purity was verified by protein gel electrophoresis followed by coomassie staining and western blotting using anti-His (HRP) antibody (Miltenyi Biotec). Signals were generated by chemiluminescence using Clarity ECL Western Blotting Substrate (BioRad ...
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bioRxiv - Immunology 2024Quote: ... Surface staining was performed for 15 min in the presence of 1 mg/ml of Beriglobin (CSL Behring) with the following fluorochrome-conjugated antibodies titrated to their optimal concentrations: anti-CD3-FITC (Miltenyi Biotec), anti-CD4-VioGreen (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... a 24-well plate was pre-coated with anti-CD3 (OKT3) and anti-CD28 antibodies (15E8) (Miltenyi Biotec, Macquarie Park, Australia) at 1µg mL-1 on day 0 ...
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bioRxiv - Neuroscience 2024Quote: ... day 27 cultures were first enriched for motor neurons by using antibodies against PSA-NCAM and L1CAM (both from Miltenyi Biotec) [39] ...
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bioRxiv - Cell Biology 2024Quote: ... proteins from the native gel were transferred to the PVDF membrane and probed with anti-CD95 antibody (Miltenyi Biotec, clone DX2).
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bioRxiv - Cell Biology 2024Quote: ... The bead-EV complexes were resuspended in 100 µL/sample of filtered PBS and incubated with the following conjugated monoclonal antibodies from Miltenyi Biotec: mouse anti-CD9-APC antibody (Clone REA1071) ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Neuroscience 2024Quote: ... In most cases microglial cells were removed using a mini MACS separator based on an anti-rat CD11b/c antibody conjugated to magnetic microbeads according to manufacturer instructions (Miltenyi Biotec, CA) (Marek et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in SFM with 10 ng/ml VEGF (Miltenyi Biotec), 10 ng/ml BMP4 (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... Satellite cells were purified with LD columns (Miltenyi Biotec, 130-042-901) by negative selection with antibodies against CD31-PE ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Anti-TRA-1-60 MicroBeads (Miltenyi Biotec, Germany) before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... then washed twice with magnetic-activated cell sorter (MACS) buffer (Miltenyi Biotec). Cells were incubated with FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... and filtered through a 30μm cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were reconstituted in calcium- and magnesium-free 1X PBS containing 400μg/mL non-acetylated BSA at concentration of ~1000 cells/μL in 2mL LoBind Eppendorf tubes (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Adult astrocytes were isolated using Magnetic-Activated Cell Sorting (MACS; Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and CD45+ cell count on a MACSQuant VYB Flow Cytometer (Miltenyi Biotec). Mice were treated with 12mg/kg MTMox32E or vehicle (5% Kolliphor EL ...
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bioRxiv - Genomics 2020Quote: ... Cells were activated with soluble human anti-CD3 (clone OKT3, Miltenyi Biotec) and human anti-CD28 (clone 15E8 ...
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bioRxiv - Immunology 2021Quote: ... cell suspensions were counted and stained with anti-CD45 microbeads (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: ... using the untouched CD4+ T cell isolation kit II (Miltenyi Biotec, Germany). More than 98% of purified cells were routinely CD4+ ...
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bioRxiv - Cancer Biology 2020Quote: B cells were isolated from splenocyte suspensions using CD43 microbeads (Miltenyi Biotec), and plated at a density of 1×106/mL in B-cell media (DMEM high glucose with Glutamax (Gibco) ...
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bioRxiv - Physiology 2020Quote: ... ECs cells were labelled with rat anti-mouse CD31 microbeads (Miltenyi Biotec). Labelled ECs and attached cells were then isolated magnetically ...
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bioRxiv - Immunology 2020Quote: Naïve splenic CD4+ T cells were purified by magnetic separation (Miltenyi Biotec). T cells were activated by α-CD28 (5 ug/ml ...
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bioRxiv - Immunology 2021Quote: ... Lineage Cell Detection Cocktail-Biotin (Miltenyi Biotec, #130-092-613, 1:100), Anti-Biotin MicroBeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... and cell suspension was loaded onto a MACS LS Column (Miltenyi Biotec) and subjected to magnetic separation ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were passed in MS Columns (Miltenyi Biotec, Bergisch Gladbach, Germany), and only the CD11c positives cells stay attached in the columns ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were passed in MS Columns (Miltenyi Biotec, Bergisch Gladbach, Germany), and only the ACSA-1 positives cells stay attached in the columns ...
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bioRxiv - Cancer Biology 2021Quote: Autologous patient derived T cells were isolated using CD3 MicroBeads (Miltenyi Biotec) according to manufacturer instructions ...