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Citations for Miltenyi Biotec :
1601 - 1650 of 2313 citations for Mouse Nucleoredoxin like protein 1 NXNL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... CD4+ T-cells were first isolated from HLA-DP4+ asymptomatic carriers using the Pan T-cell Isolation Kit (Miltenyi Biotec) and biotin-labeled anti-CD8 mAb (RPA-T8 ...
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bioRxiv - Genomics 2021Quote: ... BMMNC were either cryopreserved in FBS with 10% of DMSO or previously enriched for CD34+ (CD34 MicroBead Kit, Miltenyi Biotec) before cryopreservation.
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bioRxiv - Immunology 2021Quote: ... Naïve murine CD8+ T cells were isolated using negative selection (Naïve CD8 T Cell Isolation Kit, Cat no. 130-096-543, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the sample was subjected to a “clean-up” step using Dead Cell Removal kit (Miltenyi Biotec, Catalog #130-090-101). Cells were washed again and resuspended in 500 ul of 0.04% BSA/PBS and counted ...
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bioRxiv - Immunology 2020Quote: ... we modified the Pan T cell negative Isolation magnetic-activated cell sorting (MACSR) kit (Miltenyi Biotec, Cat #130-096-535) by adding anti-CD45RA biotin (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: CAR-and ΔLNGFR-positive T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Monocyte-derived dendritic cells (MDDCs) were generated from plastic adherent or directly isolated monocytes (Monocyte Isolation Kit II, Miltenyi Biotec) through culture for 6 days in Roswell Park Memorial Institute medium (RPMI-1640 ...
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bioRxiv - Immunology 2019Quote: ... and T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Naïve antigen presenting cells were isolated from an unimmunized (naïve ...
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bioRxiv - Immunology 2021Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... naïve splenic CD43− B cells were magnetically isolated using the B cell isolation kit (Miltenyi Biotec, Inc.; 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: NK cell effectors were enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion Kit (Miltenyi Biotec). Target and effector cells were plated at E:T ratio of 2:1 in a 96 well round bottom plate ...
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bioRxiv - Physiology 2021Quote: ... Dead cells were removed by using a dead cell removal kit and MS columns (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Single-cell RNA-seq libraries were made with the 10x Genomics Chromium Next GEM Single Cell 3’ Library Kit (v3.1 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed with magnetic cell-sorting (MACS) buffer (PBS supplemented with 0.5% BSA and 5 mM EDTA) and incubated with CD117 (KIT) MicroBeads (Miltenyi Biotec) on ice for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... or for scRNAseq following an additional step of dead cell removal using the MACS Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Primary murine monocytes were isolated from C57BL/6J bone marrow using the Monocyte Isolation Kit (Miltenyi Biotec, 130-100-629) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD34+ HSCs were isolated from the cord blood and perfused placental tissue by using the CD34 Microbead Kit (Miltenyi Biotec) and stored at −150°C ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were enriched magnetically by their expression of H2Kk using a MACSelect Transfected Cell Selection kit (Miltenyi Biotec, Sunnyvale, CA). Cas9-mediated indel formation at the Acvr1 or Bmpr1a locus was confirmed by T7E1 mismatch analysis on the enriched cell populations (T7E1 Nuclease – New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used immediately for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec). For plasma cell differentiation ...
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bioRxiv - Immunology 2022Quote: Splenic and mLN naïve B cells were purified using negative B cell isolation kits yielding enriched populations of 95%–98% (Miltenyi Biotec). Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec) ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Genomics 2022Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... CD8+ SP thymocytes were isolated using the CD8a+ (Ly-2) positive selection kit per manufacturer’s instructions (Microbeads, #130-117-044; Miltenyi Biotec). Flow-through resulted in the DN population ...
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bioRxiv - Neuroscience 2022Quote: ... followed by dissociation and isolation using the CD11b isolation kit according to the manufacturer’s protocol (130-092-628 and 130-126-725, Miltenyi Biotec). Primary microglia purity was examined by flow cytometry after staining by Cd11b-FITC (130-113-243 ...
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bioRxiv - Immunology 2022Quote: Treg and non-Treg cells were enriched using MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) from PBMCs of healthy donors and plated (1 × 105 cells per well ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were enriched using a magnetic-based commercial F4/80 macrophage positive selection kit (Miltenyi Biotec, MD, USA; Supplemental 3B) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated into a single cell suspension using a neural tissue dissociation kit (Miltenyi Biotec; 130-092-628). Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea ...
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bioRxiv - Microbiology 2023Quote: ... NK cells were then re-purified by negative selection using purification kit and magnetic columns according to manufacturer’s instructions to reach purities of approximately 98% (Miltenyi Biotec, MS columns and isolation kit ref no.130-115-818) ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8 T cells were isolated from the spleen of pmel mice (having CD8 T cells bearing T cell receptors specific for the gp100 antigen) by magnet-assisted cell sorting (Naïve CD8+ T cell isolation kit, Miltenyi Biotec) and stained with Cell Trace Violet (CTV ...
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bioRxiv - Molecular Biology 2023Quote: ... Hearts were mashed with pestles for 2 min and cell isolation performed according to manufacturer’s instructions (Neonatal Heart Dissociation Kit, Miltenyi Biotec). Cell suspension was centrifuged for 5 min at 600 x g and cells were resuspended in cell culture medium and plated in 22.1 mm plates ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec) into complete RPMI media ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion and tissue dissociation of the left lateral liver lobe was performed using a GentleMACS Octo Dissociator with heaters and the liver perfusion kit for mice according to the manufacturer’s protocol (Miltenyi Biotec). Hepatocytes were separated from lymphocytes by centrifugation at 50× g for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells were then obtained using a negative magnetic bead-based selection kit for pan T-cell isolation following the manufacturer’s protocol (Miltenyi Biotec). A negative selection was chosen to minimize any activation of T-cells during the selection process ...
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bioRxiv - Developmental Biology 2023Quote: ... The flow-through was then subjected to MuSC enrichment using the satellite cell isolation kit (Miltenyi Biotec 130-104-268). Once each population was isolated ...
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bioRxiv - Microbiology 2023Quote: ... Cells from the colonic lamina propria (cLP) were isolated using a Lamina Propria Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) on the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD4 naïve T cells were isolated from pooled lymph nodes and spleens using the naïve CD4 T cell isolation kit (130-104-453, Miltenyi Biotec) and stained with BioTracker 655 Red Cytoplasmic Membrane Dye (SCT108 ...
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then depleted of dead cells using a dead cell removal kit (catalog: 130-090-101, Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were strained (40 μm filters) and dead cells removed using a dead cell removal kit according to the manufacturer’s instructions (Miltenyi Biotec). Cell viability was determined using trypan blue staining and cells were counted using a haemocytometer ...
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bioRxiv - Immunology 2023Quote: OT-I cells were isolated from spleen of CD90.1 OT-I mice using Naive CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).