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Citations for Miltenyi Biotec :
1551 - 1600 of 1728 citations for Rat Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... hMCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, Cat. Nr.: 130-098-185). ADCC was measured using LDH release assay (Cytotoxicity Detection Kit (LDH ...
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bioRxiv - Systems Biology 2021Quote: ... Single-cell suspensions were obtained by enzymatically digesting the excised tumors using the Tumor Dissociation Kit and gentleMACS C system (Miltenyi Biotec, Auburn, CA). In addition to following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Primary cardiac fibroblasts and cardiomyocytes were isolated from the hearts of P2-P3 neonatal mice using a commercially available dissociation kit following manufacturer’s instructions (Miltenyi Biotec, 130-098-373). In brief ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Immunology 2021Quote: Murine naïve B cells were purified from spleen by magnetic negative selection by using Pan B Cell Isolation Kit (Miltenyi Biotec, 130-095-813) according to the manufacturer’s instructions and maintained in appropriate culture medium ...
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bioRxiv - Immunology 2020Quote: PBMCs were enriched for B cells by negative selection using a pan B cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1 X Phosphate-buffered Saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... PDX tumor chunks were minced then enzymatically digested into single cells by using a tumor dissociation Kit (Miltenyi biotec Cat# 130-095-929). Mouse and human cells were separated through magnetic separation ...
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bioRxiv - Cell Biology 2022Quote: ... After an additional 24h co-cultured cells were trypsinized and endothelial cells were purified using CD31 microbead kit (Miltenyi Biotec #130-091-935) according to manufacturer instructions.
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bioRxiv - Immunology 2022Quote: ... Single cells were depleted from both the CD4+ double positive (DP) and CD4+ single positive (SP) cells using a CD4+ (L3T4) positive selection kit (Microbeads; #130-117-043; Miltenyi Biotec, Gaithersburg, USA) flow-through (this contains both double-negative [DN] and CD8+SP cells) ...
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bioRxiv - Immunology 2022Quote: ... were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Microbiology 2022Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector:target ratio of 9:1 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples then underwent cellular isolation via CD11b magnetic bead sorting using the MACS Adult Brain Dissociation kit (130- 107-677; Miltenyi Biotec, Gaithersburg, MD) per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lung single cell suspensions were subjected to CD4+ cell positive enrichment according to kit manufacturer’s instructions (Miltenyi Biotec, cat. no. 130-097-048) before fluorochrome staining ...
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bioRxiv - Immunology 2023Quote: ... The CD4+ T cells were then enriched by negative selection using a CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2024Quote: ... NK cells were isolated by magnetic cell separation with a NK cell isolation kit and an AutoMACS Pro (Miltenyi Biotec, San Jose, CA). NK cells were expanded and activated for 14 days using mouse 10 ng/ml IL-15/IL-15Rα conjugate ...
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bioRxiv - Microbiology 2024Quote: ... human monocytes were isolated by negative selection with magnetic microbeads according to the manufacturer’s instructions (Pan Monocyte Isolation Kit) (Miltenyi Biotec, Bergisch Gladbach, Germany). A total of 3 x 106 monocytes/mL were seeded in complete RPMI medium in hydrophobic Lumox dishes (Sarstedt ...
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bioRxiv - Immunology 2024Quote: ... to obtain monocyte-derived macrophages which were purified with magnetic bead separation (CD14 negative isolation protocol, Monocyte Isolation Kit II. Miltenyi Biotec, Auburn, CA). Isolated CD14+ monocyte-derived macrophages (MDMs ...
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bioRxiv - Immunology 2024Quote: ... from a cell suspension and harvested from the crushed spleens of OT-I mice using a CD8a+ T-Cell Isolation Kit and magnetic-associated cell sorting (MACS) according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Similarly ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... we further isolated the CD34+ hematopoietic stem cells (HSCs) using magnetic-activated cell sorting according to the manufacturer’s instructions (CD34 MicroBead Kit, Miltenyi Biotec #130-046-702). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... and processed into a single cell suspension according to the manufacturers’ protocol using the adult brain dissociation kit (Miltenyi Biotec 130-107-677). Cells were Fc blocked (Biolegend #101320 ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... the negative fraction of CD8+ T cells selection was collected and incubated with the Tumor Cell Isolation Kit (Miltenyi Biotec, #130-108-339) for 15 min incubation at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: CD3+ T cells were enriched from the BM of 30 MM patients using the Pan T cell isolation kit with MS columns (both Miltenyi Biotec, Bergisch, Germany). 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector to target ratio ranging from 7.75:1 to 9:1 ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary NK cells were isolated from the spleens of mice (8 weeks old) by using the NK cell isolation kits according to the manufacturer’s instructions (Miltenyi Biotec #130-090-864). Primary NK cells were cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2023Quote: T2 and T3 cells were then depleted for CD45+ cells using the Miltenyi LD column prep kit (Miltenyi Biotec Inc., Charlestown, MA, USA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Plasmacytoid dendritic cells were isolated by negative selection from prepared PBMCs with the Human Plasmacytoid Dendritic Cell Isolation Kit-II from Miltenyi Biotec (Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were isolated from the spleens of 4T1 tumor-bearing mice using a Mouse MDSC Isolation Kit (Miltenyi Biotec, Cat# 130-094-538) and plated in RPMI1640 supplemented with 10% FBS and antibiotics ...
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bioRxiv - Immunology 2022Quote: B cells from PBMCs of uninfected controls or of COVID-19 convalescent individuals were enriched using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Developmental Biology 2023Quote: The fetal lung samples were dissociated into single-cell suspension using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec; cat # 130-110-201) and 37C Multi_tissue_dissociation B program ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant containing the resuspended proteins was used for immunoprecipitation assay using anti-GFP microbeads provided in the μMACS Epitope Tag Protein Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Approximately ...
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bioRxiv - Cancer Biology 2023Quote: Separations were performed on Control and EIF4G2KD HEC-1A cells using magnetic beads for CD133 (CD133 Microbead Kit, Miltenyi Biotec, 130-097-049), or CD44 (CD44 Microbeads Kit ...
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bioRxiv - Neuroscience 2024Quote: The assessment of expanded surface protein markers on sEV was carried out using a MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) following the manufacturer’s protocol.44 Thirty seven distinct surface marker antibodies (CD1c ...
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bioRxiv - Cancer Biology 2024Quote: Mouse cells were depleted from the xenograft tumor to enrich for human cells using Mouse Cell Depletion Kit (Miltenyi Biotec, 130-104-694) as previously described24 ...
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bioRxiv - Cancer Biology 2024Quote: Primary human monocytes were isolated from PBMCs from three donors using a negative selection classical monocyte isolation kit (Miltenyi Biotec 130-117-337) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD34+ HPCs were isolated using a CD34 MicroBead kit according to manufacturer’s instructions (magnetically activated cell sorting or MACS; Miltenyi Biotec, San Diego, CA). Pure populations of CD34+ HPCs were infected with TB40/E-WT or TB40/E-ΔUL138STOP HCMV (MOI = 2 ...
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bioRxiv - Immunology 2024Quote: ... Primary monocytes and neutrophils were isolated from mouse bone marrow using negative isolation kits (Miltenyi Biotec, 130-100-629 and 130-097-658). Cells were cultured in RPMI 1640 media (monocytes ...
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bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in MACS-buffer und T cells were MACS-sorted from suspension using a Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were mechanically and enzymatically dissociated with fire-polished Pasteur pipettes and the neural tissue dissociation kit with papain (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Lamina propria fractions were prepared by enzymatic digestion and dissociation with the Lamina Propria Dissociation Kit and GentleMacs Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Dissociated cells were collected for use in qPCR analysis ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the separation of neurons from non-neuronal cells (astrocytes, oligodendrocytes, microglia, endothelial cells, and fibroblasts) using the Neuron Isolation Kit (Miltenyi Biotec, 130-115-389) following the kitś instructions ...