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Citations for Miltenyi Biotec :
1551 - 1600 of 1685 citations for Mouse Anti Cytomegalovirus Phosphoprotein 65 Antibody 0885 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The analysis was carried out by SDS-PAGE and western blots using Anti-GFP HRP-conjugated (MACS Miltenyi Biotec), anti-FLAG®M2-Peroxidase (HRP ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5 ml of extract was incubated with 50 µl of either Anti-Myc (Miltenyi Biotec, ref. 130-091-123) or Anti-HA (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Cells expanded following patient biopsy collection were subsequently enriched for myoblasts using anti-CD56-coated microbeads (Miltenyi Biotec GmbH). Primary vials were sourced ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads (Miltenyi Biotec, Ref#130-090-855) with auto MACS pro (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: Astrocytes were isolated by magnetic assisted cell sorting (MACS) approach using the anti-ACSA2 microbeads (Miltenyi Biotec Inc., Germany). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed to remove unbound tetramer and were incubated with anti-PE or -APC microbeads (Miltenyi Biotec, Germany) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were purified by incubation with anti-CD4 microbeads and passing through a magnetic column (Miltenyi Biotec). The purity of cells was higher than 95% as determined by flow cytometry ...
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bioRxiv - Genomics 2020Quote: ... 3×108 cells were resuspended in 3 ml of FACS buffer (PBS with 10% FBS) and stained with 0.9 µg anti-ter119-PE (130-102-338; Miltenyi Biotec). Stained cells were conjugated to anti-PE microbeads (130-048-801 ...
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bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Biochemistry 2021Quote: ... with an incubation time of 3h at room temperature and using secondary labels 0.6 µL anti-c-myc-FITC (Miltenyi Biotec) and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82) ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and monocytes were positively selected using anti-CD14 microbeads (MACS Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The HER2 expression level was quantified by flow cytometry using anti-ErbB-2 (CD340) Vio® Bright FITC (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing NPCs was counted and Kupffer cells (KC) were removed using anti-F4/80 Ultrapure microbeads (Miltenyi Biotec) following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Microbiology 2021Quote: ... Non-T-cells containing monocytes and B-cells were prepared by T-cell depletion using anti-biotin MicroBeads (Miltenyi Biotec), biotin-labeled anti-CD4 mAb (OKT4 ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Germinal center B cells were enriched by staining cells with GL7-PE followed with anti-PE magnetic beads (0.5 uL/107 cells, Miltenyi Biotec). Positive enrichment of GL7-expressing cells was performed using MACS LS columns (Miltenyi Biotec).
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Surface- labelled plasma membrane (PM) microsomes were isolated by magnetic immunoaffinity using anti-FITC mAb-coated magnetic beads (Miltenyi Biotec) and concentrated by ultracentrifugation in thickwall polycarbonate tubes (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
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bioRxiv - Immunology 2021Quote: ... Lineage depletion for CD4/TCRβ/TCRγδ/CD11c/Gr1/NK1.1/Ter119/Igκ was performed using MACS anti- PE Micr-beads (Miltenyi Biotec). CD19+Igλ+CD45.1+CD45.2− (WT ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Plant Biology 2022Quote: ... Co-immunoprecipitation analyses were carried out as described recently using anti-GFP MicroBeads (Miltenyi Biotec, Germany; (Henrichs et al., 2012)) except that bands of interest were size-selected by Flamingo fluorescent gel stain (BioRad ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were resuspended in 8 ml cold PBE buffer (PBS with 0.5% BSA and 2 mM EDTA) and 2 ml anti-PE microbeads (Miltenyi Biotec) per 108 cells and incubated for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ expressing thymocytes were depleted using anti-CD4 and CD8 magnetic-activated cell-sorting (MACS) beads (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Immunology 2024Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... B cells were enriched by incubating cells with anti-CD43-biotin (S7) and magnetic enrichment with LS columns (Miltenyi Biotec). For class switching to IgG1 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended in 100 µl cold D-PBS and stained with fluorochrome-conjugated antibodies recognizing human CD11b and GFAP (Miltenyi Biotec, 130-113-810 and 130-123-846) as described above ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Cancer Biology 2021Quote: ... and cocultures were separated with the optimized amount of Anti-Fibroblast MicroBeads (40 μL, for up to three 100 mm Petri Dishes, Miltenyi Biotec) according to the manufacturer’s protocol to reach minimal contamination in each compartment (Fig ...
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bioRxiv - Immunology 2020Quote: CD4+ cells from the spleen of mice were purified using LS positive selection magnetic-activated columns (MACs) and anti-CD4 (L3T4) beads (Miltenyi Biotec). CD4+ MACs sorted cells were further purified by fluorescence activated cell sorting using a FACS Aria (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: The immunomagnetic isolation of oligodendrocytes from Stag2f/y and Stag2f/y;Cre P12-P14 pups was conducted using anti-O4 microbeads (Miltenyi Biotec) according to a published protocol (Flores-Obando et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Monocytes were isolated from PBMCs using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (130-050-201; Miltenyi Biotec). CD14-positive monocytes were differentiated in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 20 min at RT with 250 μL of the Inside Fix solution then washed and stained in 100 μL of the Inside Perm solution containing PE anti-TNF (clone cA2, Miltenyi Biotec) and APC anti-IL-1β (clone REA1172 ...
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bioRxiv - Developmental Biology 2021Quote: ... Stem cell marker expression was validated in isolated cell lines by flow cytometry using anti-SSEA4-FITC (Cat. # 130-098-371, Miltenyi Biotec).
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
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bioRxiv - Cancer Biology 2019Quote: ... Fresh BM aspirates from MM patients were routinely depleted from malignant plasma cells using anti-CD138-coated magnetic beads (Cat. #130051301 Miltenyi Biotec) and used for RNA sequencing and functional assays.
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bioRxiv - Immunology 2021Quote: ... were obtained by density gradient centrifugation and monocytes were subsequently purified by immunomagnetic separation using anti CD14-conjugated magnetic microbeads (Miltenyi Biotec) according to the manufacture’s protocol and as previously published (23) ...