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Citations for Miltenyi Biotec :
1551 - 1600 of 1703 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Cell samples were fluorescently stained with Anti-ACSA-2-PE (clone: IH3-18A3) (130-102-365, Miltenyi Biotec), examined by a BD FACSCanto II flow cytometer ...
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bioRxiv - Bioengineering 2023Quote: ... cells were resuspended in 300 µL of sorting buffer per 107 cells and anti-PE MicroBeads (Miltenyi Biotec) for 20 min at 4°C with constant agitation ...
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bioRxiv - Immunology 2024Quote: ... Murine neutrophils were isolated using an Anti-Ly-6G Microbeads kit (Miltenyi Biotec Cat. No. 130-120-337) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cells were again split with Accutase and MACS sorted using anti-CD144 microbeads (Miltenyi Biotec, #130-097-857) onto another Geltrex-coated plate ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were suspended in 0.5-1.0mL staining media and incubated with 2μl/bone anti-CD117 (cKit) magnetic beads (Miltenyi Biotec) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: Human Tregs were enriched from peripheral blood mononuclear cells with anti-CD25 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany), and were sorted via FACSAria as nTreg (CD4+CD25highCD127−CD45RA+ ...
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bioRxiv - Cell Biology 2021Quote: ... The hiPSC-CMs were incubated with the conjugated antibodies cTnT-Vioblue or cTnT-FITC (1:50, Miltenyi Biotec, #130-120-402 or #130-119-575). All antibodies were diluted in permeabilization medium (medium B ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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bioRxiv - Immunology 2020Quote: Peritoneal B1a cells were obtained through negative magnetic-activated cell separation with a cocktail of antibodies depleting other than B1a cells achieving more than 90% purity in isolation (Miltenyi Biotec B.V., Utrecht, The Netherlands). B1a cells were subsequently counted and plated in IMDM medium (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were then magnetically sorted to enrich CD14+ cells using microbeads conjugated to a monoclonal human CD14 antibody (Miltenyi Biotec; Cat No. 130-050-201). Cryopreserved Beagle canine CD14+ ...
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bioRxiv - Cancer Biology 2023Quote: ... holoclones and non-holoclones were divided and sorted by flow cytometry for stem-like cells following CD44 staining with CD44 antibody (Miltenyi Biotec Cat no 130-095-180). Sorted CD44high holoclones were used to isolate RNA to quantify stem cell marker expression.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed in the sorting buffer and resuspended in the same buffer with anti-FITC Microbeads (Miltenyi Biotec), and incubated on a rotor for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... CD133 and HER2 expression on the tumor cell lines were detected using anti-CD133 (130-098-826, Miltenyi Biotec) and anti-HER2 (BMS120Fl ...
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bioRxiv - Neuroscience 2020Quote: ... The same method was adjusted to obtain oligodendrocyte cultures using anti-O4-MicroBeads (Miltenyi Biotec, Cat# 130-096-670). The immortalized mouse astrocyte line C8D30 (ATCC ...
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bioRxiv - Cancer Biology 2020Quote: ... CD11b myeloid cells were purified from PBMCs by positive selection using the anti-CD11b microbeads and columns (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2020Quote: ... and TCRβ+CD138+ and TCRβ+CD138− cells were further separated with anti-PE magnetic MicroBeads (Miltenyi Biotec, Auburn, CA). After three washes with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the cell pellet re-suspended in 20 μl of anti-PE magnetic-activated cell sorting (MACS) microbeads (Miltenyi Biotec) with 80 μL separation buffer and incubated in the dark at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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bioRxiv - Cell Biology 2021Quote: ... CD11b myeloid cells were purified from PBMCs by positive selection using the anti-CD11b microbeads and columns (Miltenyi Biotec). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting samples were analysed by SDS-PAGE and immunoblotted using anti-GFP-HPR (Miltenyi Biotec, Bergisch Gladbach, Germany) and anti-HA-peroxidase (Roche Applied Science ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5 ml of extract was incubated with 50 µl of either Anti-Myc (Miltenyi Biotec, ref. 130-091-123) or Anti-HA (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed to remove unbound tetramer and were incubated with anti-PE or -APC microbeads (Miltenyi Biotec, Germany) for 15 minutes on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Cells expanded following patient biopsy collection were subsequently enriched for myoblasts using anti-CD56-coated microbeads (Miltenyi Biotec GmbH). Primary vials were sourced ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads (Miltenyi Biotec, Ref#130-090-855) with auto MACS pro (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: Astrocytes were isolated by magnetic assisted cell sorting (MACS) approach using the anti-ACSA2 microbeads (Miltenyi Biotec Inc., Germany). Briefly ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were purified by incubation with anti-CD4 microbeads and passing through a magnetic column (Miltenyi Biotec). The purity of cells was higher than 95% as determined by flow cytometry ...
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bioRxiv - Neuroscience 2024Quote: ... then astrocytes were isolated using the anti-ACSA-2 magnetic cell sorting kit and protocol (catalog #130097678, Miltenyi Biotec). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Genomics 2020Quote: ... 3×108 cells were resuspended in 3 ml of FACS buffer (PBS with 10% FBS) and stained with 0.9 µg anti-ter119-PE (130-102-338; Miltenyi Biotec). Stained cells were conjugated to anti-PE microbeads (130-048-801 ...
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bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and monocytes were positively selected using anti-CD14 microbeads (MACS Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The HER2 expression level was quantified by flow cytometry using anti-ErbB-2 (CD340) Vio® Bright FITC (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing NPCs was counted and Kupffer cells (KC) were removed using anti-F4/80 Ultrapure microbeads (Miltenyi Biotec) following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Non-T-cells containing monocytes and B-cells were prepared by T-cell depletion using anti-biotin MicroBeads (Miltenyi Biotec), biotin-labeled anti-CD4 mAb (OKT4 ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Germinal center B cells were enriched by staining cells with GL7-PE followed with anti-PE magnetic beads (0.5 uL/107 cells, Miltenyi Biotec). Positive enrichment of GL7-expressing cells was performed using MACS LS columns (Miltenyi Biotec).
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Surface- labelled plasma membrane (PM) microsomes were isolated by magnetic immunoaffinity using anti-FITC mAb-coated magnetic beads (Miltenyi Biotec) and concentrated by ultracentrifugation in thickwall polycarbonate tubes (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
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bioRxiv - Immunology 2021Quote: ... Lineage depletion for CD4/TCRβ/TCRγδ/CD11c/Gr1/NK1.1/Ter119/Igκ was performed using MACS anti- PE Micr-beads (Miltenyi Biotec). CD19+Igλ+CD45.1+CD45.2− (WT ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were resuspended in 8 ml cold PBE buffer (PBS with 0.5% BSA and 2 mM EDTA) and 2 ml anti-PE microbeads (Miltenyi Biotec) per 108 cells and incubated for 15 minutes at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Co-immunoprecipitation analyses were carried out as described recently using anti-GFP MicroBeads (Miltenyi Biotec, Germany; (Henrichs et al., 2012)) except that bands of interest were size-selected by Flamingo fluorescent gel stain (BioRad ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ expressing thymocytes were depleted using anti-CD4 and CD8 magnetic-activated cell-sorting (MACS) beads (Miltenyi Biotec), according to the manufacturer’s instructions ...