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Citations for Miltenyi Biotec :
1501 - 1550 of 1919 citations for Mouse Protein DEK DEK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... macrodissected HCC nodules and control liver samples were dissociated as single-cell suspensions using the Liver Dissociation kit (Miltenyi Biotec) and the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were enriched from bone marrow using a negative selection immuno-magnetic isolation kit according to the manufacture’s protocol (Miltenyi Biotec). Enriched neutrophils were suspended in PBS with 1% FBS and incubated with mouse FC block at a concentration of 1 µg/million cells for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells from the spleen of Foxp3-mRFP mice were enriched using a CD4 negative selection kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: CD34+ magnetic cell isolation was performed on the PBMCs according to the manufacturer’s protocol for the Direct CD34+ progenitor cell isolation kit (Miltenyi Biotec), to enrich for haematopoietic progenitor cells ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from PBMC using Human CD4+ T Cell Isolation Kits (Miltenyi Biotec, Inc., Bergisch Gladbach, Germany). Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec) ...
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bioRxiv - Genomics 2024Quote: Adrenal gland neoplasm samples were dissociated with 25-30 ul of enzyme D Multi Tissue Dissociation Kit (MTDK) (Miltenyi Biotec) or enzyme A Neural Tissue Dissociation Kit P (NTDK ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Perfused right cortex and hippocampus were dissociated together using the Adult Brain dissociation kit (Miltenyi Biotec cat. #130-107-677) and gentleMACS Octo Dissociator (Miltenyi Biotec cat ...
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bioRxiv - Immunology 2024Quote: IFN-γ production of CD8+ T cells was measured by adapting a commercially available IFN-γsecretion assay kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... mice were transcardially perfused with PBS and tissue dissociated with the Neural Tissue Dissociation kit (P) (Miltenyi Biotec, 130-092-628) in C-tubes (Miltenyi ...
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Immunology 2021Quote: ... for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS) T-cell subset Isolation Kits (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD4+ and CD8+ cells according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were minced with a scalpel and then enzymatically digested with a Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929, Germany) on a gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... naïve B cells were isolated from the spleens of mice using the B cell isolation kit (Miltenyi Biotec: 130-090-862) and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final ...
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bioRxiv - Immunology 2021Quote: ... dead cells were removed from our 24h activation sample (∼50% viability) using a MACS® Dead Cell Removal Kit (Miltenyi Biotec). In brief ...
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bioRxiv - Genomics 2020Quote: ... Brain slices were dissociated mechanically using a gentleMACS™ Octo Dissociator and enzymatically using the Adult Brain Dissociation kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... for no longer than 1 h before their enzymatical and mechanical dissociation with the Whole Skin Dissociation kit for human material (Miltenyi Biotec) and the Gentle MACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell suspensions were then filtered through 70-µm cell strainers (Falcon) and depleted of apoptotic and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... Para-tumour lung tissue samples from metastatic cancer or fibrosis patients were processed using a tumour dissociation kit (MACS Miltenyi Biotec) to isolate immune and epithelial cells ...
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bioRxiv - Pathology 2019Quote: ... Increased ratios of MDSCs and nonMDSCs were co-cultured with splenic T cells isolated with Pan T Cell Isolation Kit II (Miltenyi Biotec) and stained with 1 μM Carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... The monocytic cell fraction was isolated from the hPBMC fraction via negative selection using the MACS pan-monocyte isolation kit (Miltenyi Biotec) according to the supplier’s instructions ...
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bioRxiv - Immunology 2019Quote: B220+ B cells were recovered from bone marrow mononuclear cells from WT or CCR7−/− mice by negative selection using a MACS microbeads isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Autologous or allogenic CD3+ T cells used in T cell co-culture experiments were isolated using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instruction.
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... cells were purified with magnetic cell sorting (MACS) from healthy donor PBMCs using the CD1c (BDCA1) DC isolation kit (Miltenyi Biotec). To obtain a CD1c+CD14- population ...
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bioRxiv - Immunology 2021Quote: ... Untouched peripheral blood cDC1 and cDC2 (cDCs) and pDCs were obtained from PBMC after negative immunomagnetic separation with the Myeloid Dendritic Cell Isolation kit (Miltenyi Biotec) and the Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2020Quote: ... the population of IFNγ secreting cells was identified using the IFNγ Secretion assay detection kit (PE) (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral neutrophils were isolated by negatively removing all contaminating cells using the MACSxpress Neutrophil Isolation Kit (Miltenyi Biotec, 130-104-434) and BD Pharm Lyse buffer (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumor-bearing hemispheres and corresponding hemispheres from naïve animals were dissociated enzymatically to obtain a single-cell suspension with a Neural Tissue Dissociation Kit with papain (Miltenyi Biotec) or 0.5 mg/mL DNase I (DN25 ...
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bioRxiv - Neuroscience 2020Quote: ... P1 and P2 were re-suspended in PBS and myelin was depleted from both fractions according to the instructions of a commercial myelin-removal kit (Miltenyi Biotec), before sorting MF-CA3 synaptosomes from myelin-depleted P1.
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bioRxiv - Neuroscience 2019Quote: ... or left untransduced for 4 days prior to lysis in mitochondria isolation kit lysis buffer (1×107 cells/mL; Miltenyi Biotec) with protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Micro-dissected dentate gyri were cut into pieces with a scalpel blade and enzymatically dissociated using the Neural Tissue Dissociation Kit P (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were processed as previously described 54 and CD4+ T cells were enriched with the CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Mouse CD8a+ isolation kit (Cat no.-130-104-075) and Mini Separator Columns (Cat no.-130-042-201) were purchased from Miltenyi Biotec. H-2Kb OVA Tetramer-SIINFEKL-PE (Cat no.-TS-5001-1C ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Monocyte-and lymphocyte-enriched PBMC suspensions were sorted using Isolation kits and Depletion Column Type LS (Miltenyi Biotec Inc., Auburn, CA) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells were isolated by ficoll density gradient centrifugation (Dakewe) and T cells were purified using the Pan T Cell Isolation Kit (Miltenyi Biotec), and stimulated with CD3/CD28 T cell Activator Dynabeads (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells were resuspended with 100 µl of Inside Perm Buffer (Inside Stain Kit, Miltenyi Biotec). Eight µl of anti-GFAP and 2.5 µl of anti-Iba1 were added to the cell suspensions and mixed by gently pipetting ...
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bioRxiv - Cell Biology 2022Quote: ... The CD8+ T cells were purified from PBMCs using negative selection bead binding (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec). The following compounds were used at the indicated concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... The piece of cerebellum was dissociated into a single cell suspension by a gentleMACS Octo Dissociator within a gentleMACS C Tube in combination with the Brain Tumor Dissociation Kit (P) (Miltenyi Biotec) following all associated manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... autologous T cells were isolated from patient NSCLC lesions or from healthy donor PBMCs using the Pan T-cell Isolation Kit (Miltenyi Biotec). Autologous T cells were co-cultured with either ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue of single mice was digested with trypsin and DNase according to the guidelines of the Neural Tissue Dissociation Kit (T) (Miltenyi Biotec) using a Gentle MACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533 ...