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Citations for Miltenyi Biotec :
1451 - 1500 of 3110 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... thymocytes were isolated from adult mice by negative magnetic selection using biotinylated anti-CD4 and anti-CD8 antibodies and streptavidin (SAV) microbeads (Miltenyi Biotec). To isolate fetal progenitor cells ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Neuroscience 2021Quote: ... and stained with fluorophore conjugated antibodies (CD11b-AF700, CD45-FITC, BD Pharmingen 557690 and 553080, C5aR-PE, CD68-PE and CD107a-PE, Miltenyi Biotec 130-106-174 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... slides were incubated for 1 h at room temperature in the dark with a cardiac troponin-T antibody conjugated to APC (130-120-543; Miltenyi Biotec) prepared at a 1:100 dilution in PBS ...
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bioRxiv - Plant Biology 2022Quote: ... The blots were then incubated at room temperature with the monoclonal α-GFP antibody (1:5000) (Miltenyi Biotec, 130-091-833), α-RFP antibody (1:5000 ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec, and employed at concentrations recommended by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA) and stained with 1 μl of an APC-conjugated CD21 specific antibody (clone: HB5; 130-101-739, Miltenyi Biotec). The cells were incubated at 4 °C in the dark for 20 min and washed with and resuspended in 200 μl FACS staining buffer ...
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bioRxiv - Immunology 2020Quote: ... For CECs or mock depletion samples were stained using anti-CD71 or isotype control biotin-conjugated antibody and fractioned using streptavidin linked magnetic beads (Miltenyi Biotec) according to our previous reports 24–26,31,34 ...
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bioRxiv - Genomics 2021Quote: ... with fluorescent APC-conjugated anti-CD14 and PE-conjugated anti-CD16 antibodies (Cat#130-091-243 and Cat #130-091-245, respectively, Miltenyi Biotec). Samples were then analyzed on a MACSQuant Analyzer 10 benchtop flow cytometer (Miltenyi Biotec).
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bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for 30 min on ice by fluorochrome-conjugated monoclonal antibodies (mAbs) for either the 8-color MACSQuant Analyzer (Miltenyi Biotec), or the 14-color LSRFortessa™ X-20 flow-cytometers (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
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bioRxiv - Immunology 2023Quote: ... at a final concentration of 1 µg/mL in the presence of a blocking CD40 antibody (1 µg/mL; Miltenyi Biotec) and CD28 antibody (1 µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... Samples were divided into equal parts (250 μL each) and incubated with 50 μL of magnetic microparticles coated with monoclonal HA or MYC antibodies (MACS purification system, Miltenyi Biotec) at 4°C for 1 hour under rotation (10 rpm) ...
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bioRxiv - Immunology 2024Quote: ... CD4+CD25- Tcon were isolated from the CD25- fraction obtained from the same samples by negative selection using FITC-conjugated antibodies and anti-FITC Microbeads (Miltenyi Biotec) as described above (T cell isolation) ...
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bioRxiv - Cell Biology 2022Quote: ... Magnetic enrichment was carried out on an AutoMACS using microbeads conjugated to antibodies recognizing biotin or mouse CD117 (all from Miltenyi Biotec). HSCs (CD45.2+ ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were enriched by MACS using an in-house biotinylated anti-EGFR antibody (Cetuximab, Eli Lilly and Company, Indianapolis, IN, USA) and anti-Biotin Microbeads (Miltenyi Biotec). Purity of enriched CAR modified T cells was analyzed as described above ...
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bioRxiv - Immunology 2023Quote: ... Cells were depleted of CD4+ T cells by staining with CD4 PE-conjugated antibody (RM4-4) for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec.) as described above ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2024Quote: CAR+ T-cells were purified using a biotin-conjugated AffiniPure Goat Anti-Mouse IgG F(ab’)₂ fragment specific antibody and anti-biotin microbeads (Miltenyi Biotec). In the case of dual knock-in TET2-TRAC CAR T-cells ...
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bioRxiv - Cancer Biology 2023Quote: ... human T-ALL cells were purified after labeling with anti-huCD45 PE conjugated antibody followed by immuno magnetic selection with anti-Phycoerythrin (PE) microbeads (130-048-801, Miltenyi Biotec).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Negative controls were incubated with fluorochrome-labeled irrelevant isotype control antibody (REA Control APC, mouse cod. 130-113-446 Miltenyi Biotec).
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bioRxiv - Cell Biology 2024Quote: ... 100 μL of each sample was removed and set aside as representative “crude mitochondrial fraction.” The remaining 300 μL was diluted to 2 mL total with ICB and combined with 60 μL of superparamagnetic microbeads coated with mouse IgG1 antibodies against GFP (#130-091-125; Miltenyi Biotec). These samples were then placed on a rotating wheel at +4°C for 1 hr ...
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bioRxiv - Immunology 2024Quote: ... a 24-well plate was pre-coated with anti-CD3 (OKT3) and anti-CD28 antibodies (15E8) (Miltenyi Biotec, Macquarie Park, Australia) at 1µg mL-1 on day 0 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein purity was verified by protein gel electrophoresis followed by coomassie staining and western blotting using anti-His (HRP) antibody (Miltenyi Biotec). Signals were generated by chemiluminescence using Clarity ECL Western Blotting Substrate (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Developmental Biology 2021Quote: ... Satellite cells were purified with LD columns (Miltenyi Biotec, 130-042-901) by negative selection with antibodies against CD31-PE ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Anti-TRA-1-60 MicroBeads (Miltenyi Biotec, Germany) before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec), then stained with the I-A/E-APC antibody (MHCII ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... then washed twice with magnetic-activated cell sorter (MACS) buffer (Miltenyi Biotec). Cells were incubated with FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... and filtered through a 30μm cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were reconstituted in calcium- and magnesium-free 1X PBS containing 400μg/mL non-acetylated BSA at concentration of ~1000 cells/μL in 2mL LoBind Eppendorf tubes (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Adult astrocytes were isolated using Magnetic-Activated Cell Sorting (MACS; Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and CD45+ cell count on a MACSQuant VYB Flow Cytometer (Miltenyi Biotec). Mice were treated with 12mg/kg MTMox32E or vehicle (5% Kolliphor EL ...
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bioRxiv - Genomics 2020Quote: ... Cells were activated with soluble human anti-CD3 (clone OKT3, Miltenyi Biotec) and human anti-CD28 (clone 15E8 ...