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Citations for Miltenyi Biotec :
101 - 150 of 2657 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... transferred in gentleMACS C-tubes containing RPMI/DMEM media and kit enzymes (tumor dissociation kit, Miltenyi Biotec, Gladbach, Germany) and were broken down using the gentleMACS ...
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bioRxiv - Genomics 2021Quote: ... we used NS medium containing media hormone mix (MHM) medium (KOHJIN-Bio) supplemented with 2% MACS NeuroBrew-21 (Miltenyi Biotec), 20 ng/ml basic FGF (PEPROTECH) ...
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bioRxiv - Developmental Biology 2020Quote: Neonatal primary cardiac cultures were isolated from P0 pups with a neonatal dissociation kit (Miltenyi Biotec, 130-098-373) and homogenizer (gentleMACS) ...
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bioRxiv - Neuroscience 2020Quote: ... The same method was adjusted to obtain oligodendrocyte cultures using anti-O4-MicroBeads (Miltenyi Biotec, Cat# 130-096-670). The immortalized mouse astrocyte line C8D30 (ATCC ...
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bioRxiv - Synthetic Biology 2023Quote: ... Overnight cultures were diluted in 1:100 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells11 ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were maintained in a feeder-free format on Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec) with daily feeding ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Cell Biology 2024Quote: ... where NM is a mixture of 50% [v/v] DMEM:F12 + 50% [v/v] MACS Neuro Media (Miltenyi Biotec 130-093-570) + 0.5x GlutaMax (Thermo 35050061 ...
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bioRxiv - Cell Biology 2020Quote: ... The iPSC lines were cultured on tissue culture plastic coated with vitronectin (0.5 µg/cm2) in StemMACS iPS-Brew XF (Miltenyi Biotec). Pluripotency was validated by three lineage differentiation potential and Epi-Pluri-Score analysis ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Late-stage Dd2 parasites in RBCs were magnetically purified from 5% sorbitol-synchronized cultures using MACS LD columns (Miltenyi Biotec) and seeded at 0.8-million RBCs/well in a Seahorse miniplate which was precoated with Cell-Tak cell and tissue adhesive (Corning) ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Genetics 2023Quote: ... HSCs were harvest from the culture supernatant and were purified by MACS using CD34 MicroBeads (Miltenyi Biotec, 130-046-702).
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bioRxiv - Microbiology 2023Quote: An asynchronous parasite culture was washed in RPMI medium and then passed through a MACS LD magnet column (Miltenyi Biotec). NF54attB parasites complete a replication cycle in 44 to 48h under our culture condition and older parasites (>28h old ...
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bioRxiv - Neuroscience 2021Quote: hiPSCs were cultured and maintained undifferentiated in a chemically defined growth media (StemMACS™ iPS-Brew XF medium; Miltenyi Biotec, Paris, France) onto growth-factor-reduced Matrigel™ (Corning)-coated plates (8,6µg/cm2) ...
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bioRxiv - Cancer Biology 2020Quote: ... Following incubation with the enzymes, the sample was resuspended in media (80% RPMI 1640, 20% FBS) and filtered through MACS® SmartStrainers (70 µM; Miltenyi Biotec). The resulting single cell suspension was centrifuged at 300 × g for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: Human AML and CB cells were seeded for 10-12 days in methylcellulose-based semi-solid medium (StemMACS HSC-CFU Media complete with EPO, Miltenyi Biotec, Germany) for assessment of the frequency of colony formation ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were washed with 2% FACs buffer (PBS with 2% FBS) prior to resuspension in TexMACS complete media (130-097-196, Miltenyi Biotec, USA), supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... cultures of the two parasite lines for comparison were (i) synchronized to 80% late schizonts using MACS purification columns (Miltenyi Biotec) (ii ...
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bioRxiv - Immunology 2022Quote: ... in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). UCB or BM CD34+ HSPC subsets were directly seeded by FACS in the corresponding plates and cultured for 10 (bulk cultures ...
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bioRxiv - Immunology 2022Quote: ... in 96-well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). CD34+ HSPCs isolated from the BM of xenografted mice sacrificed between weeks 1-5 after grafting were directly seeded by FACS in the 96-well plates and cultured for 7 or 14 days before quantification of lineage outputs ...
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bioRxiv - Cancer Biology 2022Quote: ... excised tumors (200 mg) were dissociated in RPMI media containing enzyme cocktail mix from the mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA) and placed into gentleMACS dissociators per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Immunology 2024Quote: ... B cells and T cells (Miltenyi Biotec) were used ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured regularly on tissue culture plastic (TCP) coated with vitronectin (0.5 µg/cm2) in StemMACS iPS-Brew (Miltenyi Biotec, Bergish Gladbach, Germany) and passaged regularly using intermittent Ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were filtered with a 70 μm cell strainer and dead cell removal was performed on single cell suspensions (Miltenyi Biotec) and live tumor cells were frozen in 90% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Immunology 2022Quote: ... Alveolar-like MDMs were generated by differentiating CD4+ monocytes on tissue culture plates into macrophages in the presence of 50 ng/ml of human M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days ...
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bioRxiv - Neuroscience 2022Quote: ... the culture medium was replaced with a neural maintenance medium (NMM) supplemented with 1 µM StemMACS™ LDN-193189 (Miltenyi Biotec, #130-106-540) and 10 µM SB431542 (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were isolated by magnetic activated cell sorting (CD14+ Cell Isolation Kit; Miltenyi Biotec). 2×10⁵ monocytes were then differentiated into M1-type macrophages cells by incubation with 1000 U/ml GM-CSF (granulocyte macrophage-colony stimulating factor ...
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bioRxiv - Cancer Biology 2022Quote: ... Red cell lysis and dead cell removal (Miltenyi Biotec) was carried out prior to generation of single cell droplets with 10X Genomics Chromium Controller ...
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bioRxiv - Genomics 2020Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec), and cells were counted again to determine final viable cell numbers.
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bioRxiv - Genomics 2019Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec) and cells were counted again using an automatic cell counter.
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bioRxiv - Cancer Biology 2024Quote: ... the cell suspension was enriched for viable cells using the dead cell removal kit (Miltenyi Biotec) following the manufacturers protocol ...
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bioRxiv - Genetics 2020Quote: ... NK cell populations using cell-type specific kits (Miltenyi Biotec). For NK cells ...
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bioRxiv - Immunology 2022Quote: ... Cells were then magnetically enriched for total CD4+ T cells (Stemcell EasySep CD4+ T cell Enrichment kit or Miltenyi Biotec CD4+ T cell Isolation Kit). Enriched CD4+ T cells were stained with surface antibodies for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Magnetic cell sorting of CD45+ cells was performed using the MACS cell separation microbeads and reagents (Miltenyi Biotec). Total RNA was extracted with the RNeasy mini kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec) to lyse blood cells ...
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bioRxiv - Immunology 2022Quote: ... Total aortic CD3+ T-cells and CD3- cells were isolated using the Pan T cell kit (Miltenyi Biotec).
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bioRxiv - Microbiology 2021Quote: ... dead cells were removed from the 1 mL cell suspension using a dead cell removal kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Autologous naïve T cells and NK cells were purified by magnetic-activated cell sorting (MACS®; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... single cell suspension was enriched for live cells using Dead Cell Removal kit (130-090-101; Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec) according to the manufacturer’ s protocol before staining for FACS sorting was performed.
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bioRxiv - Immunology 2022Quote: ... dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec). Live cells were resuspended in Advanced DMEM/F-12 medium containing 17.51mM D-glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse cells were removed with Mouse Cell Depletion kit (Miltenyi Biotec). Tumor cells were seeded 1000 cells/well in organoid medium in 384-well plates (PrimeSurface® ...