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Citations for Miltenyi Biotec :
101 - 150 of 157 citations for Recombinant Rat Il1r1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended and incubated in MACS buffer containing Fc blocker and CD11c MicroBeads (Miltenyi Biotec) according to manufacturer instruction ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-EXO70B2 and free-GFP as a control were isolated using the μMACS GFP-tagged protein isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted cells were treated with 50 ng/mL recombinant stem cell factor (SCF; Miltenyi Biotec), 5 μM imatinib mesylate (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...
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bioRxiv - Cell Biology 2024Quote: ... The enzyme mix from Neonatal Heart Dissociation Kit mouse and rat (Miltenyi Biotec) was added to the tube and placed into the gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Cell Biology 2021Quote: ... either MNCs or CD34 enriched cells were thawed and washed and stained with FC block (Miltenyi Biotec) for 10 min and washed again ...
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bioRxiv - Immunology 2023Quote: ... Livers sections were washed again and blocked using 1% Fc Block (MACS, Miltenyi Biotec, Auburn, CA, USA) and 5% BSA in HBSS during 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Immunology 2023Quote: ... before dissociating using an Adipose Tissue Dissociation Kit for Mouse and Rat (Miltenyi Biotec), using a gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... 50 ng/ml recombinant human granulocyte macrophage colony–stimulating factor (rhGM-CSF) (130-093-865, Miltenyi Biotec), and 50 ng/ml rh interleukin (IL)-4 (130-093-921 ...
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bioRxiv - Immunology 2020Quote: ... in combination with or without recombinant interleukin-4 (IL-4, Miltenyi Biotec, catalogue number: 130-094-061) for 24h ...
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bioRxiv - Immunology 2023Quote: iMSC-like cells were acquired by stimulating ADSCs with recombinant human IL-1β (0.5ng/ml, Miltenyi Biotec) overnight ...
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bioRxiv - Immunology 2023Quote: ... and rested overnight with TexMACS medium supplemented with 100 IU/ml of recombinant human IL-2 (Miltenyi Biotec).
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bioRxiv - Neuroscience 2022Quote: ... Microglia and myelin debris were subsequently removed through a mouse anti-CD45 (Invitrogen)-coated dish and myelin removal beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was performed on 500µl blood samples from healthy donors with or without Fc fragment receptor blockers (Miltenyi Biotec). Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Immunology 2021Quote: ... Human recombinant interleukin-7 (IL-7) and interleukin-15 (IL-15) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Retronectin (Recombinant Human Fibronectin ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were subsequently stained with IL-17-FITC (1:20, rat, Miltenyi Biotec, 130-102-262), CD-3 APC/cy7 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Immunology 2019Quote: ... (at 1:20-1:100 dilutions depending on the antibody) in the presence of human Fc receptor blocking reagent (1:10) (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and rinsed once with PBS and blocked in blocking buffer (PBS with 50% FCS, FcR Blocking Reagent 1:150 (Miltenyi Biotec) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Neuroscience 2023Quote: Primary astrocytes were isolated from P0/P1 rat pup cerebral cortices using vendor established protocols (Miltenyi Biotec) and seeded on coated plates (30,000 cells/well in 96 well or 1 million cells/well in 6-well) ...
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bioRxiv - Neuroscience 2023Quote: ... Neural cells were incubated with CD11b+ magnetic microbeads (Miltenyi Biotec, CD11b/c Microbeads, rat, 130-105-634) and CD11b+ cells positively selected through a magnetic column to enrich for microglial cells ...
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bioRxiv - Biophysics 2023Quote: ... buffer (2 mM EDTA and 0.5% fetal calf serum (FCS)) and microglia were isolated by positive selection using CD11b microbeads (Miltenyi Biotec, Gladbach, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated to human monocyte-derived macrophages (hMDMs) by incubation with 100 ng/ml of recombinant human macrophage colony-stimulating factor (rhMCSF, Miltenyi Biotec) for 6 days at 37°C with 5% CO2 in a humidified atmosphere ...
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bioRxiv - Neuroscience 2022Quote: ... and 1:10 50nm-magnetic-bead antibody solution against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523). Nerve fibroblasts express the marker CD90.1 ...
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bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μl of 50nm-magnetic-bead antibodies against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523) and incubated for 15 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and monocytes were magnetically sorted with magnetic microbeads coupled with antibodies directed against CD14 (Miltenyi Biotec #130-050-201). Monocytes were then seeded on glass coverslips at 1.5×106 cells/well in six-well plates in RPMI 1640 (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...
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bioRxiv - Systems Biology 2019Quote: Cells were washed once with PBS and stained with CD11b-APC or isotype control (Rat IgG2b-APC, Miltenyi Biotec) antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Developmental Biology 2020Quote: ... The separation of STO cells from rat spermatogonial stem cells was carried using the Magnetic activated cell sorting (MACS) from Miltenyi Biotec.