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Citations for Miltenyi Biotec :
101 - 150 of 163 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in 180 μl artificial seawater containing 0.05% BSA and we added 20 μl of CD4 antibody (Miltenyi Biotec) conjugated with magnetic beads ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... and filtered resuspended nuclei with 20 um strainer (pre-separation filter; catalog # 130-101-812; Miltenyi Biotec; Bergisch Gladbach, Germany). We fluorescently labeled nuclei with propidium iodide and then quantified them with a DeNovix CellDrop automated cell counter ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Immunology 2024Quote: ... media with 3% heat-inactivated human serum (Seralab, Sussex, UK) and 20 ng/mL human recombinant IL-2 (Miltenyi Biotec). BE-CAR7 products and CART19 control cells were made as previously described using pCCL-CAR7 or pTT-CAR19 vector ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of 0.2% Tween-20/1X PBS containing 1 μL of PE-Foxp3 (Miltenyi Biotec cat# 130-111-678) was added and cells were incubated for 30 min in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... and stimulated for 18 h with IL-2 (20 ng/ml; National Cancer Institute Preclinical Repository) and/or IL-12 (10 ng/ml; Miltenyi Biotec) cytokines ...
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bioRxiv - Cell Biology 2020Quote: ... was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution, Miltenyi Biotec). Beads bound to EVs were applied onto a magnetic column and Thy1-negative EVs that passed through the column were collected.
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bioRxiv - Bioengineering 2021Quote: ... we suspended pelleted PBMCs in the sorting buffer (80 μL for 107 cells) with CD3 magnetic microbeads (20 μL for 107 cells) from Miltenyi Biotec for 15 min at 4 °C according to the capacity of the microbeads ...
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bioRxiv - Immunology 2020Quote: ... Cells were then washed three times and cultured with 1 ng/mL rhIL-15 or 20 IU/mL rhIL-2 (Miltenyi Biotec). After 3 days ...
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bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Microbiology 2022Quote: ... Stained and MACS bead-bound bacteria unbound to magnet plate were collected (20∼40 μl) and passed through MACS molecular columns (Miltenyi Biotec) (one sample/column ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei pellets were washed tree times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μl of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were successively filtered through nylon filters (100 µm and 20 µm) followed by a percoll gradient and MACS sorting with CD45 Microbeads (Miltenyi Biotec). Cells were stained with APC anti-human EpCAM antibody (Biolegend ...
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bioRxiv - Physiology 2021Quote: ... The muscle was removed from the -80 °C freezer and transferred to -20 °C where it was minced into pieces of 1-2 mm before being transferred to gentleMACS M-tubes (Miltenyi Biotec) tubes kept on ice ...
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended and incubated with 80 µL of MACS buffer and 20 µL of anti-IgM microbeads (Miltenyi Biotec) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... adjusted at 5×105 cells/ml and cultured 2-5 additional days in cell culture medium supplemented with 20 ng/ml IL-2 (Miltenyi Biotec) before being used in the experiments.
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bioRxiv - Immunology 2022Quote: ... cells were washed with 2% FBS containing PBS and incubated with 1:20 FcR blocking reagent (Cat. #130-059-901, Miltenyi Biotec) in 2% PBS for 10 min at RT ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Microbiology 2023Quote: ... 0.01% BSA and 40 U/ml RNase inhibitor) and filter through 20 µm pre-separation filters (Miltenyi Biotec, 130-041-407) and were centrifuged with a swing bucket at 500xg ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei pellets were washed three times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μL of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Immunology 2023Quote: ... Cells were depleted of CD4+ T cells by staining with CD4 PE-conjugated antibody (RM4-4) for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec.) as described above ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Stained and MACS bead-bound bacteria unbound to the magnet were collected (20∼40 μl) and passed through MACS molecular columns (Miltenyi Biotec) (one sample/column) ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng ml−1 mouse recombinant vascular endothelial growth factor and 20 ng ml−1 human recombinant fibroblast growth factor-2 (all from Miltenyi Biotec) as described 85 and plated on pre-coated (Collagen I (10 µg ml−1 Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... approximately 1×107 cells/80 µL of Magnetic Bead Buffer were incubated with anti-PE-magnetic-activated cell sorting MicroBeads (20 µL/1×107 cells; Miltenyi Biotec, UK) for another 20 min ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Following incubation with the enzymes, the sample was resuspended in media (80% RPMI 1640, 20% FBS) and filtered through MACS® SmartStrainers (70 µM; Miltenyi Biotec). The resulting single cell suspension was centrifuged at 300 × g for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the cell pellet were re-suspended in 90μl HBSS (+20% FBS) and incubate with 10μl CD31 microbeads (130-097-418, Miltenyi Biotec, Bergisch GladbacH, Germany) in a FACS tube for 15 mins on ice ...
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bioRxiv - Neuroscience 2020Quote: ... and homogenized mechanically into respective Gentle MACS C tubes containing 50 µL enzyme P + 1900 µL buffer Z and 20 µL of Buffer Y + 10 µL of Enzyme A (Miltenyi Biotec, Auburn, CA). Samples were passed through a 70 µm cell strainer (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... were processed and re-suspended in 80 µl MACS buffer (2% FBS, 2 mM EDTA in PBS) and 20 µl CD117 MicroBeads (MACS Miltenyi Biotec, Surrey, UK) to enrich LSK cells as per the manufacturers protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Anti-Biotin MicroBeads (Miltenyi Biotec, # 130-115-389) and incubated for 10 minutes at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Non-Neuronal Cells Biotin-Antibody Cocktail (Miltenyi Biotec, # 130-115-389) and incubated for 5 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The suspension was homogenized in a Dounce (loose pestle, 20 strokes) homogenizers and filtered through 100 µm MACS SmartStrainers (Miltenyi Biotec, United States). After incubation on ice for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 µl of the nuclei-containing interphase was collected and passed through a 20 µm filter (#130-101-812, Miltenyi Biotec, Bergisch Gladbach, Germany). Partially ...
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bioRxiv - Developmental Biology 2021Quote: ... Differentiation was induced when cells reached 80-90% confluency using BPEL medium (Ng et al, 2008) supplemented with 20 ng/mL Activin-A (Miltenyi Biotec, #130-115-012), 20 ng/mL BMP4 (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
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bioRxiv - Microbiology 2023Quote: ... Conidia suspensions were prepared by rinsing the plates with sterile distilled water and passed through a 20-µm cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany) to eliminate residual mycelium ...
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bioRxiv - Bioengineering 2024Quote: ... The cell pellet was resuspended in 80 µL of FACS buffer and 20 µL of the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268). The cells were incubated for 15 min at 4 °C ...