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Citations for Miltenyi Biotec :
101 - 150 of 601 citations for 6 Boc 1 oxa 6 azaspiro 3.3 heptan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Molecular Biology 2020Quote: ... 3% human serum (Seralab) +20 ng/ml human recombinant IL-2 (Miltenyi Biotec) and activated with TransAct reagent (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... 100 ng/ml of CD3-specific antibody (OKT-3; Miltenyi Biotec, Gladbach, Germany), and cultured in tissue culture flasks (TPP ...
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bioRxiv - Immunology 2023Quote: ... injected with 3 μg of anti-mouse CD45-FITC antibodies (Miltenyi Biotec, Germany) retro-orbitally and left for 3 min prior to euthanasia by cardiac puncture ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with MACSima™ Running Buffer (Miltenyi Biotec, 130-121-565) before nuclear staining was performed with DAPI (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... After 3 washes with Auto-MACS rinsing solution (Miltenyi Biotec #130-091-222) containing 0.5% BSA (Miltenyi Biotech #130-091-376) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were blocked with 3% Bovine serum albumin (BSA, Miltenyi Biotec #130-091-376) in PBS for 1 hour ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotec) or the AttuneNxT acoustic focusing cytometer (4 lasers ...
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bioRxiv - Immunology 2021Quote: ... pDCs were cultured in completed RPMI medium with 20 ng/ml IL-3 (Miltenyi Biotec). RAW264.7 cells were purchased from American Type Culture Collection and cultured in DMEM complemented with 10% FBS.
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bioRxiv - Cell Biology 2022Quote: ... and with MACS Multi-tissue dissociation kit 3 (Miltenyi Biotec Ca No. 130–110-204) at day 30 and 50 ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded 2 or 3 days before transfection experiment by MACSfectin reagent (Miltenyi Biotec), to achieve cell density recommended by the supplier.
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bioRxiv - Cell Biology 2021Quote: ... 3-5% parasitemia) were purified using magnetic activated cell sorting (VarioMACS™ Separator, Miltenyi Biotec). Importantly ...
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bioRxiv - Cancer Biology 2022Quote: ... and stimulated for activation for 3 days supplemented with 20ng/μL IL-2 (Miltenyi Biotec) and CD3/CD28 Dynabeads Human T-Activator (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... and then activated with 0.5 mg/mL of anti-CD3 (clone: OKT-3; Miltenyi Biotec) and anti-CD28 antibodies (clone ...
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bioRxiv - Molecular Biology 2022Quote: Healthy and CLL splenic B cells were isolated from one-year-old WT and Eμ–TCL1 mice by positive B-cell selection with CD19 magnetic beads (CD19 MicroBeads, Miltenyi Biotec, cat. 130-121-301). Briefly ...
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bioRxiv - Microbiology 2023Quote: MEFs were seeded at 5.104 cells per well in 24-well plates one day before stimulation and treated with 300 IU/mL IFNα (Miltenyi Biotec Cat#130-093-131) and incubated for up to 24 hours.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng/ml hFlt3-Ligand and 20 ng/ml hIL-3 (all obtained from Miltenyi Biotec, 130-094-013 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone: 15E8, Miltenyi Biotec), 25 U/mL human IL-7 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 3×105 T cells were plated per condition in 150 μl of TexMACS medium (Miltenyi Biotec) without cytokines ...
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bioRxiv - Neuroscience 2020Quote: ... To specify the differentiating cells into sensory neurons 3 µM CHIR 99021 (Miltenyi Biotec, Bergisch Gladbach, DE), 10 µM SU5402 (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... cDCs were positively selected from PBMCs with CD141 (BDCA-3) MicroBead Kit (130-090-512, Miltenyi Biotec) by MACS technology (Figure Supplementary 6C) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in presence of 3 μM CHIR-99021 and 20 ng/mL HGF (Miltenyi Biotec) for two days to obtain the GStemHep ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Immunology 2023Quote: ... and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec) and irradiated donor-mismatched PBMCs (all CAR-T cells ...
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bioRxiv - Neuroscience 2020Quote: ... single cell iPSCs were seeded at 3×105 cells/cm2 in StemMAC iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, DE) in presence of 10 µM Rock-Inhibitor Y-27632 (Cell Guidance Systems ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Immunology 2020Quote: ... A 1:1 mixture of CD3-depleted (Miltenyi Biotec) Tollip-/- (CD45.2+ ...
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bioRxiv - Cell Biology 2023Quote: ... insulin-like growth factor 1 (IGF-1, Miltenyi Biotec), FGF-2 and LDN193189 ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were spun at 400 g for 3 minutes at 4°C and washed in MACS Buffer (Miltenyi Biotec, 130-091-222) supplemented with 4% FBS (FBS ...
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bioRxiv - Immunology 2021Quote: ... CD3 (17A2; 1:400 or REA641; Miltenyi Biotec; 1:150); CD122 (TM-β1 ...
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Immunology 2019Quote: ... For genomic and transcriptomic analyses of activated LCs fresh migrated LCs from 3 donors were stimulated with TNF-α (25 ng/ml, Miltenyi Biotec, UK) for 2 ...