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Citations for Miltenyi Biotec :
101 - 150 of 318 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from splenocytes of OT-I mice on C57BL/6 background using anti-CD8a microbeads (Miltenyi Biotec), and then fluorescently labeled with carboxyfluorescein succinimidyl ester (CFSE) ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were maintained in a feeder-free format on Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec) with daily feeding ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 6 mL MACS+ buffer and evenly distributed between two LS columns (Miltenyi Biotec 130-042-401) in the presence of a magnetic field (generated by the QuadroMACS magnetic separator ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from fully grown (Day15) melanomas of C57BL/6 mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). The samples were fixed in a 2.5% glutaraldehyde solution/ 0.1M Sodium Cacodylate Buffer (SCB ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: NK cells were enriched from the spleens of C57BL/6 Kaede mice using NK Cell Isolation Kit (#130-115- 818, Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The omental tumors harvested from C57Bl/6 mice were minced and dissociated using the Human Tumor Dissociation Kit from Miltenyi Biotec, following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... naive CD4+ T cells (CD4+CD25−CD62L+) were sorted from the spleen of WT C57Bl/6 mice using a commercially available kit (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2024Quote: Control or DON-treated tumors (n = 1 per group) were harvested after 6 days of treatment and positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) before submission ...
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bioRxiv - Cell Biology 2024Quote: ... cells are dissociated with TrypLE for 6-8 minutes and non-endothelial cells were separated through MAC sorting with CD144 MicroBeads (Miltenyi Biotec). Purified endothelial cells (passage 0 ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Cell Biology 2024Quote: ... 5ml of fresh ACME solution (15% methanol/10% glacial acetic acid/10% glycerol in Milli-Q water) were added to labelled polyps in gentleMACS™ C-tubes (Miltenyi Biotec). During the ACME incubation ...
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bioRxiv - Neuroscience 2020Quote: ... for 5 minutes and Anti-Biotin MicroBeads (Miltenyi Biotec, 130-090-485) for 10 minutes at 2-8°C ...
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bioRxiv - Genomics 2021Quote: Libraries were prepared from 5 fmol miRXplore Universal Reference Pool (Miltenyi Biotec) and 200 ng total cellular RNA using fixed NEBNext-adapters or 5N-adapters ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended in the presence of anti-biotin MicroBeads (1:5; Miltenyi Biotec) and incubated for 15 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Spleen cells were incubated at 4°C with anti-CD4 (L3T4)-MicroBeads (Miltenyi Biotec) after 10 min CD4 (RM4-5)-PB ...
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bioRxiv - Developmental Biology 2023Quote: ... for 20 min at 4 °C and passed through a LS column (Miltenyi Biotec). The flow-through was collected and used for cell surface staining ...
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bioRxiv - Developmental Biology 2023Quote: ... for 20 min at 4°C and passed through a LS column (Miltenyi Biotec). The flow-through was collected and used for cell surface staining ...
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bioRxiv - Developmental Biology 2023Quote: ... for 20 min at 4°C and passed through a LS column (Miltenyi Biotec). The flow-through was collected and used for cell surface staining with the antibodies listed in Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from Day15 B16.F10 melanomas of C57BL/6 mice or α-SMAcreAtg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). 5 x 104 cells CAFs were seeded on poly-L-Lysine coated glass coverslips ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C for 30 minutes in the presence or absence of 10 ng/ml human recombinant IL-6 (Miltenyi Biotec, Germany) or IL-23 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... we added an ice-cold buffer of 5% autoMACS Rinsing Solution (Miltenyi Biotec) in 1X MACS BSA solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
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bioRxiv - Cell Biology 2024Quote: ... and SSEA-5 (+) cells were collected using an autoMACS Pro Separator (Miltenyi biotec). Genomic DNA was purified from at least 100 million cells of each sample using NucleoSpin Blood XL (Takara ...
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bioRxiv - Genomics 2023Quote: ... Up to 5×109 PBMC were incubated with anti-CD14 microbeads (Miltenyi Biotec) for 30 minutes at room temperature and magnetically enriched using the CliniMACS system (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 5 ng/mL of IL-15 (130-093-955; Miltenyi Biotec), 1X Glutamax and 100 U/mL penicillin-streptomycin.
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bioRxiv - Neuroscience 2024Quote: ... for 5 min and Anti-Biotin MicroBeads (Miltenyi Biotec, Cat # 130-090-485) for 10 min at 2-8°C ...
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bioRxiv - Immunology 2021Quote: ... and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec). Anti-CD24 antibody-bound cells were then magnetically removed ...
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bioRxiv - Bioengineering 2021Quote: ... for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec). Monocytes were cultured using Iscove’s Modified Dulbecco’s media supplemented with 10% human serum blood type AB ...
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bioRxiv - Physiology 2022Quote: ... for 20 minutes at 4°C before Auto MACS cell separation (Miltenyi Biotec, Cologne, Germany). Cells were then sorted with a BD FACSAria Fusion (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... Tumor digestion protocol #4 was performed by using the gentleMACS Octo tissue dissociator (Miltenyi Biotec) with three 10 min rounds of processing over 40 min ...
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bioRxiv - Neuroscience 2024Quote: ... M2 macrophages were generated by conditioning BMDM with IL-4 (20 ng/ml; Miltenyi Biotec) for 16-18 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... with the anti-CD304 (BDCA-4/Neuropilin-1) MicroBead kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) and identified as BDCA-2 positive ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were passaged every 5-7 days using Stem-MACS Passaging Solution (Miltenyi Biotec). Differentiation of SV20 into cardiomyocytes (iCMs ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Cancer Biology 2021Quote: ... with 5 mL of digesting enzyme included in a Tumor Dissociation Kit (Miltenyi Biotec). The tissues were then converted into single-cell suspensions using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to LS columns (Miltenyi Biotec). Subsequently ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... and the tissue was stored overnight at 4°C in MACS Tissue Storage Solution (Miltenyi Biotec). Tumor tissue was minced and dissociated using the mouse Tumor Dissociation Kit (Miltenyi Biotec ...