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Citations for Miltenyi Biotec :
101 - 150 of 696 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec) and irradiated donor-mismatched PBMCs (all CAR-T cells ...
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bioRxiv - Cell Biology 2024Quote: At specified timepoints the confluent cell cultures were enzymatically dissociated using Multi Tissue Dissociation Kit 3 (Miltenyi Biotec) and replated at 10.000 cells/cm2 in RPMI +B27 +Ins media with ROCK inhibitor Y27632 (5 µM ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs at day 40 of differentiation were dissociated with MACS Multi Tissue Dissociation Kit 3 (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor portions were minced to 3-4 mm pieces and dissociated using a Tumor Dissociation Kit (Miltenyi Biotec), followed by the clearance of red blood cells with RBC lysis buffer (eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... Cells were passaged every 5-7 days using Stem-MACS Passaging Solution (Miltenyi Biotec). Differentiation of SV20 into cardiomyocytes (iCMs ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Cancer Biology 2021Quote: ... with 5 mL of digesting enzyme included in a Tumor Dissociation Kit (Miltenyi Biotec). The tissues were then converted into single-cell suspensions using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Cell Biology 2022Quote: ... + 5 µL DNase + 0.5% heat-inactivated FCS) with 10 µL FcR blocking reagent (Miltenyi Biotec), 10 µL biotin labeled anti-CD45 antibody (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated in 5% FBS RPMI-1640 in the presence of an enzyme mixture (Miltenyi Biotec) at 37℃ for 45 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... falciparum culture (4% hematocrit, 5-10% parasitemia) was passed through an LS column (Miltenyi Biotec; previously rinsed with 2 mL culture medium ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... and matured through the addition of 5 ng/mL BDNF (Miltenyi Biotec, #130-096-286) and 10 ng/mL GDNF (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 ml of PBS and mechanically dissociated using a gentleMACS(tm) Dissociator (Miltenyi Biotec). Lysates were then plated in serial log10 dilutions onto 100×15mm Middlebrook 7H10 plates (Hardy Diagnosatics) ...
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bioRxiv - Neuroscience 2024Quote: ... The brain slices were blocked with 5% Bovine Serum Albumin (Miltenyi Biotec, 130-091-376) and 0.4% Triton X-100 (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then blocked with 5% Bovine Serum Albumin (Miltenyi Biotec, 130-091-376) and 0.4% Triton X-100 (Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Immunology 2024Quote: ... media with 3% heat-inactivated human serum (Seralab, Sussex, UK) and 20 ng/mL human recombinant IL-2 (Miltenyi Biotec). BE-CAR7 products and CART19 control cells were made as previously described using pCCL-CAR7 or pTT-CAR19 vector ...
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bioRxiv - Neuroscience 2021Quote: ... microglia proliferation was induced by adding 5 ng/ml GM-CSF (Miltenyi Biotec, 130-095-746) to the complete medium ...
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bioRxiv - Immunology 2021Quote: ... and IL-5 were enriched for live cells using a dead cell removal kit (Miltenyi Biotec) followed by CD138-APC (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... microglia proliferation was induced by adding 5 ng/ml GM-CSF (Miltenyi Biotec, 130-095-746) to the complete medium ...
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bioRxiv - Cancer Biology 2023Quote: ... We incubated with 5 µL of Annexin V-conjugated FITC (MACS Miltenyi Biotec, Lab Systems SA) at room temperature in darkness for 20 min ...
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bioRxiv - Immunology 2024Quote: ... Some of the cultures were also supplemented with 5 ng/mL of exogenous cytokines (Miltenyi Biotec) recombinant human rh-IL15 or rh-IL15+rh-IL21 ...
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bioRxiv - Genomics 2024Quote: ... we combined 300 mg of the 5 SAT biopsies into a gentleMACS C tube (Miltenyi Biotec) containing 3 ml of chilled 0.1X lysis ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... hFlt3-Ligand 100ng/mL, 130-096-479; hIL-3 60ng/mL, 130-095-069; TPO 10nM, 130-094-013, all from Miltenyi Biotec) and 1% PS during 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ cells were enriched from BM MNCs of sex-matched SF3B1-mutated MDS patients (n = 3) and healthy donors (n = 2) using CD34 Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). 4000 or 7500 cells were plated as duplicates in MethoCult (H4434 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec). All iPS lines were genotyped using an RFLP at the relevant important loci and the sequence was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... we stained batches of 5 million cells with 20 µL FOXA2-APC (Miltenyi Biotec #130-107-774) and 4 µL SOX17-PE (Miltenyi Biotec #130-111-032) ...
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bioRxiv - Genomics 2022Quote: ... cells were dissociated using TrypLE for 5 min and sorted using CD144-conjugated magnetic microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14, Miltenyi Biotec). After 3 hrs of specific peptide stimulation ...