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Citations for Miltenyi Biotec :
101 - 150 of 659 citations for 5 2 4 Dimethylphenyl 3 methyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and homogenized in STKM buffer (250 mM Sucrose, 50 mM Tris-HCl, 25 mM KCl, 5 mM MgCl2) using the GentleMACS (Miltenyi Biotec) program Protein_01 ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in PBS and cultured for 5 days in serum-free DMEM containing 50 ng/ml BDNF (Miltenyi Biotec Cat# 130-096-285 ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... perfused murine lungs were placed in a gentle MACS C tube and mechanically homogenized in 5 ml of PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec). Lung cell suspensions were lysed of RBCs ...
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bioRxiv - Immunology 2022Quote: All cell culture was performed in a humidified incubator with 5% CO2 at 37°C using cRPMI (see above for ingredients) supplemented with 150ng/mL αCD40 (clone FGK-45; Miltenyi Biotec) and 25ng/mL recombinant murine IL-4 (Peprotech ...
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bioRxiv - Molecular Biology 2024Quote: ... and both the sample and collection tubes were placed in a cold MACS Chill 5 Rack (130-092-951, Miltenyi Biotec). Both positive and negative fractions were collected using the separator ...
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bioRxiv - Molecular Biology 2023Quote: ... following which the cells were cryopreserved at a concentration of 5×106-1×107 cells/mL in CryoMACS 50 or 250 Bags (Miltenyi Biotec) for the gene edited drug product generation step.
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bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... After another incubation of 30 minutes at 37°C with 5% CO2 the samples are analysed by flow cytometry (MACS Quant, Miltenyi Biotec) and FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were stained with 5 μg/ml of propidium iodide in PBS and analyzed using a MACSquant analyzer (Miltenyi Biotec). Results were analyzed using Flowjo (https://www.flowjo.com).
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were cultured for six days at 37°C/5%CO2 in the presence of either M-CSF (50 ng/mL; Miltenyi Biotec) or GM-CSF (5 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were subcultivated by incubating the cells for 5 minutes at room temperature with StemMACS Passaging Solution XF (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Immunology 2020Quote: ... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... or 30 ng/mL interleukin-4 (IL-4; Miltenyi Biotec) (M2 ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Bioengineering 2023Quote: ... [4] (Miltenyi Biotec), and the lineage-negative NSG HSPCs were collected after MACS depletion of the lineage-positive marrow cells ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement without retinoic acid (Miltenyi Biotec) supplemented with 10 µM SB-431542 ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS™ C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS™ Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CD4 (VIT-4, PerCp, Miltenyi Biotec, VIT-4, VioBlue, Miltenyi Biotec), CD8 (RPA-T8 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CD4 (VIT-4, PerCp, Miltenyi Biotec, VIT-4, VioBlue, Miltenyi Biotec), CD8 (RPA-T8 ...
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bioRxiv - Developmental Biology 2022Quote: ... 3 μM GSK3i (Miltenyi Biotec) and 10 μM of ROCKi (Y-27632 ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were centrifuged and resuspended in a 5% fetal bovine serum in PBS (FBS/PBS) with CD133-PE (Miltenyi Biotec 130-080-801), CD44-FITC (BD Pharmingen 555478 ...
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bioRxiv - Neuroscience 2024Quote: E15.5 embryo brains were extracted and cortices were dissociated into single-cell suspension using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628). Progenitors were magnetically selected and isolated using Anti-Prominin1 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Developmental Biology 2020Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec), together abbreviated as 2i ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were added to the medium ...
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bioRxiv - Developmental Biology 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec). Cells were maintained at 37°C with 6% CO2.
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bioRxiv - Cell Biology 2020Quote: ... CHIR99021 (CH, 3 μM, Miltenyi Biotec) and leukemia inhibitory factor (LIF ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM CHIR 99021 (Miltenyi Biotec) and 150 μM LAAP (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were also added to the medium ...