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Citations for Miltenyi Biotec :
101 - 150 of 771 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... freshly harvested tissues were incubated with appropriate enzymes (Miltenyi Biotec, Table 3) followed by mechanical dissociation (gentleMACS ...
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bioRxiv - Molecular Biology 2021Quote: ... and 4 µL SOX17-PE (Miltenyi Biotec #130-111-032). We used FACS to sort populations into undifferentiated (FOXA2/SOX17 double negative ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) for B cells ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
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bioRxiv - Microbiology 2022Quote: PBMCs isolated from healthy donors (n=6) were used for CD4+T cell isolation kit (Miltenyi Biotec). CD4+T cells were treated with recombinant IL-2 ...
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bioRxiv - Microbiology 2022Quote: ... mature parasite cultures (6-8% parasitemia) were magnetically separated using magnetic columns (MACS LD columns, Miltenyi Biotec) and diluted to 1% parasitemia containing 0.5 mL fresh erythrocytes88 ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were positively sorted with beads from the spleens of C57BL/6 mice (Miltenyi Biotec). The T cells were labelled with CFSE (Invitrogen Molecular Probes) ...
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bioRxiv - Immunology 2024Quote: ... DCs were purified (>90%) from the spleens of C57BL/6 mice using MACS CD11c microbeads (Miltenyi Biotec). Freshly isolated CD8+ T cells were then activated either with anti-CD3 and anti-CD28 antibody-coated microbeads at a 1:1 bead:cell ratio (Miltenyi Biotec ...
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bioRxiv - Genomics 2024Quote: ... Each fraction was washed with BSA rinsing buffer and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-allophycocyanin MACS® MicroBeads (Miltenyi Biotec, #130-090-855, lot #5190809239). Each fraction was loaded onto an MS column (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... 3% human serum (Seralab) +20 ng/ml human recombinant IL-2 (Miltenyi Biotec) and activated with TransAct reagent (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... 100 ng/ml of CD3-specific antibody (OKT-3; Miltenyi Biotec, Gladbach, Germany), and cultured in tissue culture flasks (TPP ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with MACSima™ Running Buffer (Miltenyi Biotec, 130-121-565) before nuclear staining was performed with DAPI (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... After 3 washes with Auto-MACS rinsing solution (Miltenyi Biotec #130-091-222) containing 0.5% BSA (Miltenyi Biotech #130-091-376) ...
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bioRxiv - Immunology 2023Quote: ... injected with 3 μg of anti-mouse CD45-FITC antibodies (Miltenyi Biotec, Germany) retro-orbitally and left for 3 min prior to euthanasia by cardiac puncture ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... After 3 washes with Auto-MACS rinsing solution (Miltenyi Biotec #130-091-222) containing 0.5% BSA (Miltenyi Biotech #130-091-376) ...
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bioRxiv - Neuroscience 2021Quote: ... or 4) Oligodendrocytic (O4-APC (REA576, #130-119-982, Miltenyi Biotec)) fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2021Quote: ... and 20 ng/ml IL-4 (Miltenyi Biotec, Bergisch Gladbach, Germany) for M2-polarization ...
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bioRxiv - Immunology 2020Quote: ... at RT and permeabilized with InsidePerm at 4°C (Miltenyi Biotec) followed by incubation with anti-IL-10-PE-conjugated antibody (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 40 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) for dendritic cells ...
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bioRxiv - Bioengineering 2024Quote: ... at 4 °C for 30 min followed by MACS (Miltenyi Biotec) following manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: ... IL-4 (#130093917) and IL-13 (#130112409) were from Miltenyi Biotec, Sydney ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained for 30 min at 4°C with the following anti-human antibodies: PerCP-Vio700-conjugated REAfinity recombinant anti-CD31 (Miltenyi Biotec, clone REA730, #130-110-673, 1/20), BV421-conjugated anti-Tissue Factor/CD142 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... The EGFR-Hofbauer/fibroblast fraction was washed and incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ phycoerythrin (PE)-conjugated anti-CD10 antibody (anti-CD10-PE, Miltenyi Biotec, #130-114-5-2, lot #5190109168). The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Plates were blocked with 3% Bovine serum albumin (BSA, Miltenyi Biotec #130-091-376) in PBS for 1 hour ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotec) or the AttuneNxT acoustic focusing cytometer (4 lasers ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were sorted from pooled spleens of WT C57BL/6 mice by using mouse CD11c MicroBeads (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2020Quote: Neutrophils from bone marrow were purified from C57Bl/6 mice by negative selection using neutrophil isolation kit (Miltenyi Biotec). Purified neutrophils were cultured at 1.0-5.0 x 107 total cells and were infected or not at different ratios with RFP LmRyn metacyclic promastigotes ...
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bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with 10 ng/ml hIL-4 (Miltenyi Biotec, Germany) and treated with anti-hIL4 scFv (Creative Biolabs ...
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bioRxiv - Immunology 2024Quote: ... M-CSF + IL-4 (40 ng/ml, # 130-093-917, Miltenyi Biotec), M-CSF + IL-6 (50 ng/ml ...
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bioRxiv - Microbiology 2024Quote: ... MDDC medium was supplemented with IL-4 and GM-CSF (Miltenyi Biotec, 130-093-922 and 130-093-866 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD19 and anti-BDCA-4 microbeads (Miltenyi Biotec, Bergisch-Gladbach, Germany). Monocytes were enriched in conjunction with PDC with Fc block ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
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bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Immunology 2022Quote: ... cells were prepared from CD45.1 Thy1.2 C57Bl/6 mice by first crushing bones followed by T cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). CD45.2 Thy1.2 FoxP3/GFP+ CD4+ Treg were FACS sorted from CD8/CD19-depleted single cell suspensions from spleens and lymph nodes from CD45.2 Thy1.2 FoxP3GFP+ C57Bl/6 using a BD FACS Aria II ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Renal primary cells were obtained from 6 C57/Bl6 mice after mechanical dissociation performed using the gentleMACS dissociator (Miltenyi Biotec). About 80,000 cells were therefore seeded just after isolation into XF96 cell culture microplates (Agilent ...
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bioRxiv - Immunology 2021Quote: ... pDCs were cultured in completed RPMI medium with 20 ng/ml IL-3 (Miltenyi Biotec). RAW264.7 cells were purchased from American Type Culture Collection and cultured in DMEM complemented with 10% FBS.
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bioRxiv - Cell Biology 2022Quote: ... and with MACS Multi-tissue dissociation kit 3 (Miltenyi Biotec Ca No. 130–110-204) at day 30 and 50 ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded 2 or 3 days before transfection experiment by MACSfectin reagent (Miltenyi Biotec), to achieve cell density recommended by the supplier.
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bioRxiv - Cell Biology 2021Quote: ... 3-5% parasitemia) were purified using magnetic activated cell sorting (VarioMACS™ Separator, Miltenyi Biotec). Importantly ...
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bioRxiv - Cancer Biology 2022Quote: ... and stimulated for activation for 3 days supplemented with 20ng/μL IL-2 (Miltenyi Biotec) and CD3/CD28 Dynabeads Human T-Activator (Gibco ...
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bioRxiv - Immunology 2022Quote: ... 4×105 neutrophils were stained with AnnexinV-FITC in Binding Buffer (Miltenyi Biotec). For mitochondria analyses ...
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bioRxiv - Microbiology 2020Quote: ... and 50 ng/ml rh interleukin (IL)-4 (130-093-921, Miltenyi Biotec)] and cultured at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 4 ng/mL of recombinant basic fibroblast growth factor (Miltenyi Biotec, Germany). Cells were cultured for 2 passages at a seeding density of 5×10 cells/cm.
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bioRxiv - Cancer Biology 2023Quote: ... at 4°C and distributed at 5 mL per LS column (Miltenyi Biotec), or 5×106 BM MNC were thawed ...
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bioRxiv - Systems Biology 2023Quote: ... cells were eluted with 4 mL Whole Blood Column Elution Buffer (Miltenyi Biotec) and centrifuged at 500 g for 10 minutes at 4°C ...