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Citations for Miltenyi Biotec :
101 - 150 of 700 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet from each BM sample was resuspended in 392µl PBS/3% FBS and 8µl CD117 (cKit) MicroBeads (Miltenyi Biotec, catalog number 130-091-224) and incubated on ice for 30 mins ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended in the presence of anti-biotin MicroBeads (1:5; Miltenyi Biotec) and incubated for 15 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Cancer Biology 2024Quote: ... TYR or Mart-1/MLANA PepTivator peptide pools (15-mer peptide sequences with 11 amino acids overlap, Miltenyi Biotec) for 60 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Different lineages of in vitro cultured endMSCs (n = 3) were primed using a combination of recombinant pro-inflammatory cytokines and Toll-like receptors (TLR) ligands: IFNγ (Miltenyi Biotec Inc, San Diego, CA, USA) at 1 ng/ml and 100 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were stained using CD117-APC (Miltenyi Biotec, 130-098-207, 1:50) and CD133-PE (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Immunology 2020Quote: ... IL-5-Vio515 (Miltenyi Biotec), IL-4-PE Dazzle594 and IL-13-BV421 (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... following which the cells were cryopreserved at a concentration of 5×106-1×107 cells/mL in CryoMACS 50 or 250 Bags (Miltenyi Biotec) for the gene edited drug product generation step.
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bioRxiv - Cell Biology 2020Quote: ... and 5 ng/mL EGF (Miltenyi Biotec). Half of the medium was changed every day until the first colonies appeared ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 5 ng/ml human IL-7 (Miltenyi Biotec), and 5 ng/ml human FLT3L (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... and 5 ng/ml human FLT3L (Miltenyi Biotec) as described previously 50.
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng/mL GM-CSF (Miltenyi Biotec) at 37 °C in a humidified atmosphere with 5% CO2.
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bioRxiv - Immunology 2024Quote: ... or GM-CSF (5 ng/mL; Miltenyi Biotec) for macrophage differentiation ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were enriched by positive selection for c-Kit expression using anti-CD117 immunomagnetic beads (130-091-224, Miltenyi Biotec Inc. 1:5 dilution). Second ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Genomics 2024Quote: ... The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec, #130-105-639). The Hofbauer/fibroblast fraction was loaded onto an MS column for magnetic separation ...
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bioRxiv - Neuroscience 2022Quote: ... MACS NeuroBrew-21 with retinoic acid with final concentrations noted (10 mL, MACS Miltenyi Biotec, 130-093-566), PD0332991 (20 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml human FGF-2 (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2021Quote: ... and PepTivator® SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec, 15 mers with 11 amino acid overlap) covering the entire SARS-CoV-2 S protein ...
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bioRxiv - Microbiology 2020Quote: ... 5 million units per kg recombinant mouse IFNα (Miltenyi Biotec) were injected intravenously ...
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bioRxiv - Genomics 2024Quote: ... Each fraction was washed with BSA rinsing buffer and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-allophycocyanin MACS® MicroBeads (Miltenyi Biotec, #130-090-855, lot #5190809239). Each fraction was loaded onto an MS column (Miltenyi Biotec ...
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bioRxiv - Genomics 2024Quote: ... The EGFR-Hofbauer/fibroblast fraction was washed and incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ phycoerythrin (PE)-conjugated anti-CD10 antibody (anti-CD10-PE, Miltenyi Biotec, #130-114-5-2, lot #5190109168). The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Cell Biology 2024Quote: ... 5ml of fresh ACME solution (15% methanol/10% glacial acetic acid/10% glycerol in Milli-Q water) were added to labelled polyps in gentleMACS™ C-tubes (Miltenyi Biotec). During the ACME incubation ...
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bioRxiv - Neuroscience 2020Quote: ... for 5 minutes and Anti-Biotin MicroBeads (Miltenyi Biotec, 130-090-485) for 10 minutes at 2-8°C ...
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bioRxiv - Genomics 2021Quote: Libraries were prepared from 5 fmol miRXplore Universal Reference Pool (Miltenyi Biotec) and 200 ng total cellular RNA using fixed NEBNext-adapters or 5N-adapters ...
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bioRxiv - Neuroscience 2020Quote: ... we added an ice-cold buffer of 5% autoMACS Rinsing Solution (Miltenyi Biotec) in 1X MACS BSA solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
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bioRxiv - Cancer Biology 2023Quote: ... at 4°C and distributed at 5 mL per LS column (Miltenyi Biotec), or 5×106 BM MNC were thawed ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 5 ng/mL of IL-15 (130-093-955; Miltenyi Biotec), 1X Glutamax and 100 U/mL penicillin-streptomycin.
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bioRxiv - Cell Biology 2024Quote: ... and SSEA-5 (+) cells were collected using an autoMACS Pro Separator (Miltenyi biotec). Genomic DNA was purified from at least 100 million cells of each sample using NucleoSpin Blood XL (Takara ...
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bioRxiv - Genomics 2023Quote: ... Up to 5×109 PBMC were incubated with anti-CD14 microbeads (Miltenyi Biotec) for 30 minutes at room temperature and magnetically enriched using the CliniMACS system (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 min and Anti-Biotin MicroBeads (Miltenyi Biotec, Cat # 130-090-485) for 10 min at 2-8°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were passaged every 5-7 days using Stem-MACS Passaging Solution (Miltenyi Biotec). Differentiation of SV20 into cardiomyocytes (iCMs ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Cancer Biology 2021Quote: ... with 5 mL of digesting enzyme included in a Tumor Dissociation Kit (Miltenyi Biotec). The tissues were then converted into single-cell suspensions using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...