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Citations for Miltenyi Biotec :
1401 - 1450 of 1723 citations for Rat Malonyl Coenzyme A Decarboxylase MLYCD ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) or using the EasySepTM Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Bioengineering 2023Quote: CD4+ and CD8+ T cells were then isolated from PBMCs by magnetic associated cell sorting (MACS) using the CD4+ or CD8+ human T cell isolation kit (130-096-533 or 130-096-495; Miltenyi Biotec), respectively ...
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bioRxiv - Microbiology 2023Quote: ... NK cells were isolated from PBMCs by negative selection using the human NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). NK cells were cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Immunology 2023Quote: ... or Kdm6aflox/flox LckCre (UTXTCD) 8- to 12-week-old female donor spleens using the CD4+ T cell Isolation Kit II (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PDOX-derived cells were FACS-sorted (P8, nude control brain)23 or MACS-purified (remaining PDOXs) with a Mouse Cell Depletion kit (Miltenyi Biotec)21 ...
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bioRxiv - Immunology 2023Quote: ... The livers were harvested and dissociated into single-cell suspensions using the Liver Dissociation Kit on a GentleMACS Octo Dissociator (Miltenyi Biotec). The cell suspension was filtered through a 70 μm strainer ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Tumor and bladder tissue were harvested and processed into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed using RBC lysis buffer before being magnetically sorted using the CD8+ T cell isolation kit (Catalog # 130-096-543, Miltenyi Biotec). Neutrophil preparation ...
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bioRxiv - Cancer Biology 2022Quote: ... Both CD45 nondepleted and CD45-depleted single-cell suspensions were depleted of dead cells using a Dead Cell Removal kit (catalog no. 130-090-101; Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Part of the single-cell suspension was depleted of CD45+ cells to enrich for tumour cells using a CD45 MicroBeads kit (catalog no. 130-045-801; Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Bioengineering 2023Quote: ... Lung progenitor cultures at day 14 of differentiation were detached as described above and fixed and permeabilized using the transcription factor staining buffer kit (Miltenyi Biotec). After washing with flow buffer (PBS w/o containing 1.0% BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Genetics 2023Quote: PBS-perfused brains were cut into small pieces and dissociated using the Adult Brain Dissociation Kit and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Cancer Biology 2023Quote: Primary BM mononuclear cells isolated from MM patients or healthy donors were enriched in CD138+ PCs using magnetic sorting with the CD138 Microbead Kit (Miltenyi Biotec). Cells were plated in 48-well plates previously seeded with human BM-derived mesenchymal cells.
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bioRxiv - Synthetic Biology 2023Quote: ... were isolated using Ficoll gradient centrifugation and human T cells were obtained by magnetic sorting using the eHuman Pan T cell isolation kit (Miltenyi Biotec). Once isolated ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells from the sample were purified by magnetic bead separation using the CD4+CD62L+ isolation kit (Miltenyi Biotec). Cells were plated in 96-well plates coated with anti-CD3 antibody (5μg/ml ...
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bioRxiv - Immunology 2023Quote: Autologous CD8 T cells were obtained from frozen PBMCs from the same donor using the CD8+ Microbeads human T cell isolation Kit (Miltenyi Biotec). To monitor cell proliferation ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC were differentiated into cells of all three germ layers using the StemMACS Trilineage Differentiation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly ...
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bioRxiv - Genetics 2023Quote: ... gentle and rapid generation of single-cell suspensions was achieved by using a modified version of the procedure of a mouse Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Systems Biology 2024Quote: ... we first processed a portion of the cell harvest solution using the dead cell removal kit (Miltenyi Biotec 130-090-101) to remove any cell debris to avoid any free RNA from lysed cells from getting mixed in with mRNA to be extracted from live cells ...
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bioRxiv - Synthetic Biology 2024Quote: The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Brain tissue was dissociated into a single-cell suspension by enzymatic digestion using the Adult Brain Dissociation Kit P (# 130-107-677, Miltenyi Biotec) and the gentleMACS™ Octo Dissociator (# 614 130-096-427 ...
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bioRxiv - Neuroscience 2024Quote: ... according to the manufacturer’s instructions including a red blood cell removal and a debris removal step (Adult Brain Dissociation Kit P, # 130-107-677, Miltenyi Biotec). Isolated cells were washed twice with D-PBS (# 14040-133 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was immediately dissociated to single-cell suspension by enzymatic degradation and myelin removal using the adult brain dissociation kit and GentleMACS dissociator (Miltenyi Biotec.). The isolated tissues were placed in C-tubes ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were obtained from the lymph nodes (LNs) and spleens of OT-1 mice using a negative selection kit (130-104-075, Miltenyi Biotec). For CTL differentiation ...
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bioRxiv - Immunology 2023Quote: ... naive CD4+ T cells (CD4+CD25−CD62L+) were sorted from the spleen of WT C57Bl/6 mice using a commercially available kit (Miltenyi Biotec). Next ...
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bioRxiv - Neuroscience 2023Quote: ... a single cell suspension from the hippocampus and cortex was prepared using Neural Tissue Dissociation Kit (papain-based) (Miltenyi Biotec GmbH). After blocking with CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The single cell suspension was then depleted of dead cells and debris using magnetic-activated cell sorting (MACS, Dead Cell Depletion Kit, Miltenyi Biotec). Cells were then distributed drop-wise onto a Seq-Well microwell array preloaded with mRNA capture beads and processed as described previously5 ...
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bioRxiv - Cancer Biology 2023Quote: Primary tumour epithelial cells were obtained using the gentle MACS Dissociator and Mouse Tumor dissociation kit (Miltenyi Biotec, Bisley, Surrey, UK) following the protocol recommended for ‘Dissociation of Tough Tumors’ ...
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bioRxiv - Immunology 2023Quote: ... IEC/IEL single cell suspensions were prepared as above and dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec) using LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were then purified by negative magnetic cell sorting using mouse CD8α+ T-cell isolation kit (130-104-075; Miltenyi Biotec) according to the manufacture’s protocols ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 1×106 vehicle or ACCi-conditioned OT-1 T cells were infused into B16-OVA tumor bearing mice 10 days after tumor challenge and tumors were harvested 7 days later using the Mouse Tumor Dissociation Kit and gentleMACS Octo dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: ... cerebellum of WT and KIKO mice was dissociated to single-cell suspension using adult brain dissociation kit from MACS Technology and using GentleMACS (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Skeletal muscle myoblasts derived from WT and KO ADAMTSL1 mice were isolated using the Satellite Cell Isolation Kit MACS protocol (Miltenyi Biotec), as described previously34.
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bioRxiv - Immunology 2023Quote: ... B cells were first enriched from the splenocyte suspension by MACS (magnetic activated cell sorting) using the Pan B cell Isolation Kit (Miltenyi Biotec), followed by staining for FACS (fluorescence activated cell sorting) ...
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bioRxiv - Cell Biology 2023Quote: ... One ovary from each mouse was dissociated using a Multi Tissue Dissociation Kit 1 (cat# 130110201, Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions to create a single-cell suspension in D-PBS (cat# 14287080 ...
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bioRxiv - Physiology 2024Quote: ... Brains without the cerebellum and olfactory bulbs were collected and dissociated by using an Adult Brain Dissociation Kit and a gentleMACS Octo Dissociator with Heater (Miltenyi Biotec). Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage ...
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bioRxiv - Genetics 2024Quote: ... were harvested and T cells were isolated by magnetic separation with the human Pan T Cell isolation kit and stimulated using TransAct (both Miltenyi Biotec). Cells were then cultured in TexMacs medium (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... The lung was perfused with 1X PBS and processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). Blood was treated with RBC lysis buffer for five minutes ...
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bioRxiv - Cell Biology 2024Quote: ... CB mononucleated cells were enriched for CD34+ cells using the human CD34 MicroBeads Kit following manufacturer instructions (130-046-703, Miltenyi Biotec), assessed for purity (≥70% CD34+ cells) ...