Labshake search
Citations for Miltenyi Biotec :
1351 - 1400 of 1491 citations for Mouse anti Legionella pneumophila LPS F029 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
-
bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
-
bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
-
bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
-
bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... Fresh BM aspirates from MM patients were routinely depleted from malignant plasma cells using anti-CD138-coated magnetic beads (Cat. #130051301 Miltenyi Biotec) and used for RNA sequencing and functional assays.
-
bioRxiv - Immunology 2021Quote: ... were obtained by density gradient centrifugation and monocytes were subsequently purified by immunomagnetic separation using anti CD14-conjugated magnetic microbeads (Miltenyi Biotec) according to the manufacture’s protocol and as previously published (23) ...
-
bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
-
bioRxiv - Microbiology 2020Quote: ... BMDCs were enriched by depletion of BMMs using biotinylated α-F4/80 antibody (eBioscience, RRID: AB_466657) and anti-biotin conjugated magnetic beads (Miltenyi Biotec). The BMDCs in the flow through were positively selected using α-CD11c magnetic beads according to the manufacturer’s protocol (Mil405 tenyi Biotec).
-
bioRxiv - Immunology 2019Quote: ... or sorted using a FACSAria II (Becton Dickinson) flow cytometer after antibody staining and lineage selection or depletion using anti-biotin beads and LS columns (Miltenyi Biotec). Data was analysed using FlowJo software (Version 8.8.7 ...
-
bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
-
bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and incubated in 2% FBS in PBS with anti-ROR1-APC antibody (1:25, #130-119-860, Miltenyi Biotec) on ice for 30 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
-
bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... loaded with biotinylated TCR and then for 1-2 additional rounds with tetramers of TCR and anti-AlexaFluor647 microbeads (Miltenyi Biotec). TCR tetramers were created by adding TCR to tetrameric AlexaFluor647-conjugated streptavidin (produced in-house ...
-
bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
-
bioRxiv - Immunology 2022Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec) 85 ...
-
bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The NCAM+ Merkel cells in our cell suspension were bound by anti-PSA-NCAM microbeads (Miltenyi Biotec, Cat. 130-097-859) and extracted with the MACS® Column system ...
-
bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from splenocytes of OT-I mice on C57BL/6 background using anti-CD8a microbeads (Miltenyi Biotec), and then fluorescently labeled with carboxyfluorescein succinimidyl ester (CFSE) ...
-
Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
-
bioRxiv - Immunology 2020Quote: ... For CECs or mock depletion samples were stained using anti-CD71 or isotype control biotin-conjugated antibody and fractioned using streptavidin linked magnetic beads (Miltenyi Biotec) according to our previous reports 24–26,31,34 ...
-
bioRxiv - Immunology 2019Quote: Human monocytes were purified from healthy donor peripheral blood mononuclear cells (PBMCs) by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). moDCs were obtained by culturing monocytes for 5 days with IL-4 and GM-CSF (Peprotech) ...
-
bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
-
bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
-
bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
-
bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
-
bioRxiv - Immunology 2022Quote: B cells were purified from the spleens of mice (aged 2–7 months of age) through negative selection with anti-CD43 magnetic beads (Miltenyi Biotec) and subsequently cultured in RPMI 1640 media (Life Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... The pelleted MVs were incubated with 10 μl of Biotin-conjugated anti-CD105 (Miltenyi Biotec cat# 130-094-916, RRID: AB_10828348) followed by adding 10 μl of anti-Biotin microbeads (MiltenyiBiotec cat# 130-094-256 ...
-
bioRxiv - Cancer Biology 2024Quote: ... The purity of the NK cell population was validated by flow cytometry using PE-Vio 770 anti-human CD56 (1:50; REA196 clone; Miltenyi Biotec) and FITC anti-human CD3 (1:50 ...
-
bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
-
bioRxiv - Immunology 2022Quote: ... Cultures were activated with anti-CD2/CD3/CD28 coated beads at a bead to cell of ratio of 1:1 (Miltenyi Biotec). Cocultures were stained for viability with the LIVE/DEAD Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2024Quote: ... T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec, San Jose, CA). Next ...
-
bioRxiv - Molecular Biology 2024Quote: ... the cell pellets were subjected to a 15-minute staining procedure in the dark using Anti-CD11b Magnetic Microbeads (130–093-634, Miltenyi Biotec) on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cell suspension was sorted for CD31+ endothelial cells using anti-CD31 MicroBeads (Miltenyi Biotec, 130-097-418; San Diego, CA) and a magnetic cell isolation system (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
-
bioRxiv - Immunology 2024Quote: ... staining and single-cell sorting of PvAMA1-specific IgG+ MBCs were performed as follows: B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec) and stained with mouse anti-human CD20 (PE-Cy5.5 ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
-
bioRxiv - Immunology 2024Quote: ... CD4+CD25- Tcon were isolated from the CD25- fraction obtained from the same samples by negative selection using FITC-conjugated antibodies and anti-FITC Microbeads (Miltenyi Biotec) as described above (T cell isolation) ...
-
bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec)53 ...
-
bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
-
bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched using human anti-CD8 MicroBeads and the automated magnetic activated cell sorting (autoMACS) system (both Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... spleen or bone marrow cells were stained for 30 min on ice with Anti-CD23 Magnetic Microbeads (130-098-784, Miltenyi Biotec) or Anti-F4/80 Magnetic Microbeads (130-110-443 ...
-
bioRxiv - Immunology 2022Quote: ... to 1×108/ml and Ly6G+ cells were isolated according to the anti-Ly6G MicroBead Kit (#130-092-332, Miltenyi Biotec). The cells were labeled with biotinylated anti-Ly6G antibodies (anti-Ly6G-biotin ...
-
bioRxiv - Immunology 2023Quote: ... T cells were stimulated by the addition of 100 IU/mL IL-2 (Proleukin) and anti-CD3/CD28 stimulation using loaded MACSiBead particles (Miltenyi Biotec) in a proportion of 1:1 of beads to cell ...
-
bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...