Labshake search
Citations for Miltenyi Biotec :
1351 - 1400 of 1924 citations for Mouse Anti Canine Parvovirus 2 Antibody 2A10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... T cells were magnetically separated from other splenocytes using the Mouse Pan-T Cell Isolation Kit II (Miltenyi Biotec, Cat# 130-095-130), labeled with CellTrace™ CFSE (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2022Quote: ... excised tumors (200 mg) were dissociated in RPMI media containing enzyme cocktail mix from the mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA) and placed into gentleMACS dissociators per manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
-
bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
-
bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: MDSCs were isolated from the spleens of 4T1 tumor-bearing mice using a Mouse MDSC Isolation Kit (Miltenyi Biotec, Cat# 130-094-538) and plated in RPMI1640 supplemented with 10% FBS and antibiotics ...
-
bioRxiv - Immunology 2024Quote: ... Primary monocytes and neutrophils were isolated from mouse bone marrow using negative isolation kits (Miltenyi Biotec, 130-100-629 and 130-097-658). Cells were cultured in RPMI 1640 media (monocytes ...
-
bioRxiv - Cancer Biology 2019Quote: ... The live cell fraction was then incubated with CD45 magnetically-labeled antibody (Miltenyi Biotec, 130-052-301) and passed through a LS magnetic column (Miltenyi Biotec ...
-
bioRxiv - Immunology 2019Quote: ... The diluted blood was labeled with antibodies directed against Ter119 (FITC, TER-119 1/30 Miltenyi Biotec), CD71 (PE ...
-
bioRxiv - Immunology 2019Quote: ... CD56+ cell fractions were collected using antibody-coated magnetic beads (Dynabeads,Thermo Fisher Scientific; CD56, Miltenyi Biotec). Antibody purified fractions were then extracted for DNA and RNA using the AllPrep DNA/RNA/miRNA Universal Kit according to the manufacturer’s instructions (QIAGEN).
-
bioRxiv - Immunology 2022Quote: Monocytes were isolated from freshly collected PBMCs using CD14 antibody-conjugated microbeads and magnetic columns (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... and positively selected using 10 uL PDGFRα antibody-coated microbeads (1:10; Miltenyi Biotec #130-101-547). PDGFRα+ stoma was placed into magnetic separation MS columns (Miltenyi Biotec #130-042-021 ...
-
bioRxiv - Cell Biology 2020Quote: ... and primary antibodies against CD45-FITC (12μl/sample, Cat nb. 130-114-567, Clone 5B1, Miltenyi Biotec), CD31-FITC (4μl/sample ...
-
bioRxiv - Bioengineering 2020Quote: ... CD3 and CD28 monoclonal antibodies (beads T cell activation/Expansion Kit, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) were co-cultured with WJ-MSCs (A/ WJ hMSCs ...
-
bioRxiv - Cancer Biology 2023Quote: ... To detect CD4, an antibody already labelled with FITC (130-113-775, clone REA623) from Miltenyi biotec was used at a concentration of 1:100 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Antibodies were diluted with Running Buffer in a MACSwell™ Deepwell Plates (Miltenyi Biotec, 130-126-865) in a total volume of 1050 µl ...
-
bioRxiv - Molecular Biology 2023Quote: ... Antibodies against CD14 (#130-110-583) and HLA-DR (#130-111-943) were obtained from Miltenyi Biotec. Dapi (#422801 ...
-
bioRxiv - Cancer Biology 2024Quote: ... immunomagnetic microbeads decorated with recombinantly engineered antibody fragments for CD11b (Miltenyi Biotec, Cat. No. 130-049-601) were used with the MiniMACS Separation Unit (Miltenyi Biotec ...
-
bioRxiv - Molecular Biology 2023Quote: ... µMACS DYKDDDK Isolation Kits were used for anti-FLAG IPs and HA Isolation Kits for anti-HA immunopurification (Miltenyi Biotec #130-101-591 and #130-091-122). Next ...
-
bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
-
bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
-
bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
-
bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
-
bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
-
bioRxiv - Immunology 2021Quote: ... 2 mM EDTA in PBS) before positive selection of CD14+ monocytes by incubation with magnetic CD14 microbeads (Miltenyi Biotec, 130-050-201) (15 min ...
-
bioRxiv - Cell Biology 2024Quote: ... + 0.5x GlutaMax (Thermo 35050061) + 1x NEAA (Thermo 11140050) + 1x N-2 (Thermo 17502048) + 1x MACS NeuroBrew-21 (Miltenyi Biotec 130-093-566). On D3 ...
-
bioRxiv - Cell Biology 2023Quote: ... tissues were cleansed in ice-cold RPMI1640 and dissociated using the Multi-tissue dissociation kit 2 (Catalog No.130-110-203, Miltenyi Biotec, USA) following the manufacturer instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
-
bioRxiv - Immunology 2024Quote: ... both CD45.2 DKO and WT polyclonal donor marrow was depleted of mature T cells by magnetic column-based negative selection (Miltenyi Biotec MACS) using an anti-mouse CD5 biotin-conjugated antibody (Clone 53-7.3 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
-
bioRxiv - Cancer Biology 2019Quote: ... the live cell fraction was subjected to a similar MACS based isolation by application of mouse MDSC isolation kit (Miltenyi Biotec, 130-094-538). Isolated cells were washed twice with cold MACS buffer and counted with a hemocytometer and diluted in cold PBS with 0.1% BSA and 2 mM EDTA at desired densities for Drop-seq.
-
bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
-
bioRxiv - Neuroscience 2021Quote: ... and the brains were extracted and dissociated using Miltenyi Adult mouse and rat Brain Dissociation Kit and the gentleMACS Octo Dissociator (Miltenyi Biotec 130-107-677). The cells were then resuspended in PBS + 0.5% BSA and stained with DAPI to determine viability ...
-
bioRxiv - Immunology 2022Quote: Lungs were isolated and processed into single cell suspension using gentleMACS tissue dissociator and mouse lung dissociation kit (Miltenyi Biotec Ref. 130-095-927) followed by 40 μm filtration and red blood cells lysis with ACK lysis buffer ...
-
bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were purified from CD4+ T cells from pooled spleens and lymph nodes of Cd226−/− 2D2 TCR Tg mice by using mouse CD4 MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany), followed by flow cytometric sorting for CD25-CD44LoCD62LHiCD4+ cells ...
-
bioRxiv - Bioengineering 2022Quote: ... ≈1 gram of muscle tissues was dissected from the hindlimb muscles of a humanely euthanized mouse and placed into a GentleMACS dissociation tube (Miltenyi Biotec, #130-096-334). 7 mL of DMEM (Gibco ...
-
bioRxiv - Microbiology 2021Quote: Total spleen T cells were purified by negative selection with the mouse naïve CD4+ T Cells Isolation Kit (Miltenyi Biotec, Cat# 130-104-453) and LS separation columns (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in fresh media containing either 100 nM CCK or 100 nM saline vehicle control up to 72 hours with concurrent treatment with a mouse cytokine cocktail containing 50-ng/mL TNFα (Miltenyi Biotec, #139-101-687), 10-ng/mL IL-1β (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2022Quote: ... and mechanically and chemically digested into single-cell suspensions using the gentleMACS Dissociator and the mouse tumor dissociation kit by Miltenyi Biotec (130-096-730) respectively ...
-
bioRxiv - Cancer Biology 2022Quote: Thyroid lobes (8 lobes/condition/experiment) were cut in 1mm3 pieces and processed into single cells suspensions with the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) diluted in Ca2+ free DMEM (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
-
bioRxiv - Molecular Biology 2023Quote: B cells were purified from red blood cell-lysed single-cell suspensions of mouse spleens by magnetic negative selection using a B Cell Isolation Kit (Miltenyi Biotec, 130-090-862). B cells (3x105 per well in a 12-well plate ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lin- cells isolated from the bone marrow of donor mice (8–12 weeks old) via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA, USA). Lin- cells were transduced overnight at a viral multiplicity of infection (MOI ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
-
bioRxiv - Immunology 2023Quote: ... cells were spun down at 500g for 10 minutes and resuspended in MACS buffer to purify PMNs with a magnetic purification kit according to manufacturer’s instruction (Miltenyi Biotec; Neutrophil Isolation Kit, Mouse).
-
bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mice were sacrificed at predetermined timepoints and human CD45+ cells were isolated from bone marrow and spleen using the mouse cell depletion kit (Miltenyi Biotec, #130-104-694) to achieve >90% purity of human cells.