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Citations for Miltenyi Biotec :
1301 - 1350 of 1584 citations for Leptin SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... One ovary from each mouse was dissociated using a Multi Tissue Dissociation Kit 1 (cat# 130110201, Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions to create a single-cell suspension in D-PBS (cat# 14287080 ...
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bioRxiv - Physiology 2024Quote: ... Brains without the cerebellum and olfactory bulbs were collected and dissociated by using an Adult Brain Dissociation Kit and a gentleMACS Octo Dissociator with Heater (Miltenyi Biotec). Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage ...
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bioRxiv - Cancer Biology 2023Quote: The omental tumors harvested from C57Bl/6 mice were minced and dissociated using the Human Tumor Dissociation Kit from Miltenyi Biotec, following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was sectioned into pieces and a portion of tumour or normal brain was dissociated using the MACS Brain Tumor Dissociation Kit (Miltenyi Biotec). Labelled engrafted GFP+ cells were isolated from dissociated cell population using a BD FACSAria Fusion flow cytometer machine at the McGill University Health Centre Research Institute platform ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: CD4 T cells were isolated from the spleens of donor mice and purified using the mouse CD4 T Cell Isolation Kit (Miltenyi Biotec). The purity and cell count of donor CD4 T cells were assessed using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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A framework for defining mesenchymal cell types associated with murine periosteal and endosteal bonebioRxiv - Genomics 2023Quote: ... cells were depleted of hematopoietic cells and endothelial cells using a lineage depletion kit (Miltenyi Biotec, cat. no. 130-090-858) followed by CD45 ...
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bioRxiv - Microbiology 2024Quote: ... Norway) centrifugation and used for CD4+ T cell isolation by negative selection with a commercial kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2024Quote: We isolated single cells from the dissected mPFC sections using a neural tissue dissociation kit (Cat# 130-092-628, Miltenyi Biotec). After cell concentration quantified ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... human (130-050-501) combinations based on the Mini & MidiMACS™ Starting Kit (130-091-632) (all from Miltenyi Biotec, Germany) in accordance with the manufacturer’s protocols.
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bioRxiv - Developmental Biology 2024Quote: ... containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec, Cat-Nr: 130-098-373). Two mouse hearts were processed in separate C-tubes at the same time for all experiments presented in this manuscript ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was cut into small pieces and processed using Neural Tissue Dissociation kit (P)-papain (Miltenyi Biotec 130-092-628) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... supernatants were discarded and cell pellets were processed according to the manufacturers protocol for MACS of astroglial cells using the ACSA2 isolation kit (Miltenyi Biotec). Afterwards ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... human CD3+ positive selection kit (# 130-050-101), and murine CD3+ and CD8+ negative selection kits (# 130-095-130, # 130-104-075) were purchased from Miltenyi Biotec. SiR-Actin (# CY-SC001 ...
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bioRxiv - Cell Biology 2024Quote: ... Autologous CD4+ T cells were isolated from PBMCs with a negative CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec).
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bioRxiv - Genomics 2024Quote: All tumours were labelled with Annexin specific MACS beads using the Dead Cell Removal Kit (Miltenyi Biotec Cat# 130-090-101) following the manufacturers’ instructions and dead cells were removed by passing the labelled cells through the autoMACS® Pro (Miltenyi ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from fully grown (Day15) melanomas of C57BL/6 mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). The samples were fixed in a 2.5% glutaraldehyde solution/ 0.1M Sodium Cacodylate Buffer (SCB ...
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bioRxiv - Cancer Biology 2024Quote: ... 3.5 µL 1 mg/mL Hoechst 33342 dye and 399 µL of permeabilization buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec).
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were detached from the mice and approximately 150 mg of tumor tissue was enzymatically and mechanically digested using the Mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single cell suspension ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were stained with APC-eFluor780-conjugated anti-c-Kit (clone: 2B8) and fractionated using anti-APC magnetic beads and LS columns (both Miltenyi Biotec). c-Kit+ cells were then stained with combinations of antibodies as described in Supplemental Table 1 ...
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bioRxiv - Immunology 2024Quote: Single cells suspension from tumour-bearing lungs described previously were used for CD8+ T cells sorting using immunomagnetic sorting using CD8α+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells were isolated from peripheral blood mononuclear cells obtained from healthy donors using Human CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manual ...
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bioRxiv - Immunology 2024Quote: ... OT-I CD8 T (OT-I) cells were enriched by negative selection using a CD8a+ T cell isolation kit (Miltenyi Biotec), commonly reaching a purity of 90-98% as determined by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the minced auricular lymph nodes using the CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: YFP+ Treg cells were isolated by sorting lymph node samples pre-enriched for CD25+ cells using a mouse CD25 Microbead kit (Miltenyi Biotec) on a FACSAria (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... was used to purify NK cells and the Human Naïve Pan T Cell Isolation kit (Miltenyi Biotec; CAT#130-097-095) was used to purify T cells ...
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bioRxiv - Immunology 2023Quote: ... were isolated from the spleen and lymph nodes of FoxP3-aPIK3CD mice using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec). Genomic DNA was isolated from these cells using the AllPrep DNA/RNA Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Immunology 2023Quote: Splenocytes or small intestinal IELs isolated from QFLTg or OT-1 mice were depleted of non-T cells using Pan T isolation Kit II (Miltenyi Biotec). The negatively selected T cells were then labeled with 5µM Cell Tracing Violet (CTV ...
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bioRxiv - Cell Biology 2020Quote: EpCAM-positive cells were isolated from the differentiated endodermal cells using a magnetic cell sorting kit (MACS; Miltenyi Biotec K.K. Cologne, Germany) with the CD326 (EpCAM ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions containing the IEC fraction were diluted to 108 cells/mL in MACs buffer (0.5% BSA 2mM EDTA) before being processed with the Miltenyi Dead Cell Removal Kit (Miltenyi Biotec, Auburn, CA) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein complexes with TTL3-GFP and free GFP as a control were isolated using the μMACS and MultiMACS GFP Isolation Kits (Miltenyi Biotec, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: NK cells were purified from PBMCs from healthy donors using an NK cell isolation kit (Miltenyi Biotec, Inc., San Diego, CA, USA). NK cells were stimulated with irradiated (100 Gy ...
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bioRxiv - Biochemistry 2021Quote: Memory B cells were isolated from Peripheral blood mononuclear cells (PBMCs) derived from blood samples from COVID-19 convalescent patients using a Human Memory B cell isolation kit (Miltenyi Biotec, GE). Small pools of purified Memory B cells were seeded into 384-well plates on irradiated CD40L-expressing feeder cells for differentiation into plasma cells as described previously (41) ...
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bioRxiv - Neuroscience 2020Quote: ... The brain tissues were cut into small pieces and dissociated into single cells using a modified version of the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec, US). Myelin debris was removed using the Myelin Removal kit II (130-096-733 ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Bone marrow aspirates from healthy donor and pathological patients were enriched for plasma cells by magnetic cell separation using a human CD138 positive selection and Macs Separator kits (Miltenyi Biotec, Germany).
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bioRxiv - Microbiology 2021Quote: Primary human neutrophils were isolated via negative depletion method from human blood with MACSxpress® Whole Blood Neutrophil Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood lymphocytes (PBLs) from a healthy donor were stimulated using the T Cell Activation/ Expansion Kit (MACS,Miltenyi Biotec, USA) according to manufacturer instructions ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Harvested spleens from intramuscularly vaccinated mice were processed into single cell suspensions with a gentleMACS™ Octo Dissociator and Spleen Dissociation Kit (Miltenyi Biotec) with slight modifications to the manufacturer’s protocol ...