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Citations for Miltenyi Biotec :
1301 - 1350 of 1702 citations for Homovanillic Acid HVA CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... mouse bone marrow neutrophils were sorted from the bone marrow cell suspension using a mouse neutrophil isolation kit that enriches for CD11b+Ly6G+ cells (Miltenyi Biotec; negative selection using a cocktail that contains anti-CD5 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then immunoisolated with GFP-LC3B using 50 μL of GFP microbeads from a GFP isolation kit (Miltenyi Biotec; 130091125) added to each sample ...
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bioRxiv - Immunology 2022Quote: ... OT-1 T cells were isolated from the spleens OT-1 mice using negative selection with CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were Fc blocked with α-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... tumors from unimmunized or mice immunized with WTL+LPS+PGPC were harvested then dissociated using the tumor Dissociation Kit and the gentleMACS dissociator (Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells obtained using Ficoll density gradient centrifugation (Lymphoprep, ELITechGroup) followed by CD8+ T cell isolation (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec) on buffy coats from HLA-A2.1+ human donors after written informed consent in accordance with the Declaration of Helsinki and in agreement with institutional guidelines ...
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bioRxiv - Immunology 2022Quote: ... ratio were purified by magnetic-activated cell sorting (MACS®) according to the manufacturer’s instructions (NK cell Isolation kit; Miltenyi Biotec). Total RNA from the samples was isolated using TRIzol® reagent following the manufacturer’s isolation protocol (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: Isolated Tregs from WT or NAC1-/- mice were activated and expanded with kits (# 130-104-454 and #130-095-925; Miltenyi Biotec), then treated with cycloheximide (150 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... Further disaggregation of iCCA tissue into a single-cell solution for sequencing was completed using the MACS tumor dissociation kit (Miltenyi Biotec) with the standard tough tumor protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the mixture of unlabeled RNA and biotinylated RNA was incubated with streptavidin beads from uMACS Streptavidin kits (Miltenyi Biotec) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Primary neurons were derived from the cerebral cortex of p0-p1 mice following standard operational procedure using the neural tissue dissociation kit-postnatal neurons (Cat. 130-094-802, Miltenyi Biotec) as previously described 30 ...
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bioRxiv - Neuroscience 2022Quote: ... P10 and P20 were extracted from brains and dissociated using Adult Brain Dissociation Kit and OctoMACS Separator (both from Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were manually dissociated with a razor blade on ice and placed in the gentleMACSTM Octo Dissociator and Adult Brain Dissociation Kit (Cat# 130-107-677) (Miltenyi Biotec), supplemented with the inhibitor cocktail ...
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bioRxiv - Molecular Biology 2022Quote: Dissociation of fresh Fallopian tube epithelium was done using the Milltenyi gentleMACS Octo Dissociator with heaters and the human Tumor Dissociation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2022Quote: Treg and Tconv cells were enriched from PBMCs of patients with IBD and healthy donors using MACS CD4+CD25+ Regulatory T Cell Isolation Kit human (Miltenyi Biotec) for the co-culture experiments ...
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bioRxiv - Synthetic Biology 2024Quote: The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... we first processed a portion of the cell harvest solution using the dead cell removal kit (Miltenyi Biotec 130-090-101) to remove any cell debris to avoid any free RNA from lysed cells from getting mixed in with mRNA to be extracted from live cells ...
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bioRxiv - Neuroscience 2024Quote: ... Brain tissue was dissociated into a single-cell suspension by enzymatic digestion using the Adult Brain Dissociation Kit P (# 130-107-677, Miltenyi Biotec) and the gentleMACS™ Octo Dissociator (# 614 130-096-427 ...
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bioRxiv - Neuroscience 2024Quote: ... according to the manufacturer’s instructions including a red blood cell removal and a debris removal step (Adult Brain Dissociation Kit P, # 130-107-677, Miltenyi Biotec). Isolated cells were washed twice with D-PBS (# 14040-133 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was immediately dissociated to single-cell suspension by enzymatic degradation and myelin removal using the adult brain dissociation kit and GentleMACS dissociator (Miltenyi Biotec.). The isolated tissues were placed in C-tubes ...
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bioRxiv - Physiology 2024Quote: ... Brains without the cerebellum and olfactory bulbs were collected and dissociated by using an Adult Brain Dissociation Kit and a gentleMACS Octo Dissociator with Heater (Miltenyi Biotec). Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2024Quote: ... Norway) centrifugation and used for CD4+ T cell isolation by negative selection with a commercial kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec, Cat-Nr: 130-098-373). Two mouse hearts were processed in separate C-tubes at the same time for all experiments presented in this manuscript ...
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bioRxiv - Neuroscience 2024Quote: We isolated single cells from the dissected mPFC sections using a neural tissue dissociation kit (Cat# 130-092-628, Miltenyi Biotec). After cell concentration quantified ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... human (130-050-501) combinations based on the Mini & MidiMACS™ Starting Kit (130-091-632) (all from Miltenyi Biotec, Germany) in accordance with the manufacturer’s protocols.
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bioRxiv - Neuroscience 2024Quote: ... supernatants were discarded and cell pellets were processed according to the manufacturers protocol for MACS of astroglial cells using the ACSA2 isolation kit (Miltenyi Biotec). Afterwards ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was cut into small pieces and processed using Neural Tissue Dissociation kit (P)-papain (Miltenyi Biotec 130-092-628) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Two hundred thousand T cells per well were cultured in 96-well plates in the presence of T Cell Activation/Expansion Kit (Miltenyi Biotec) for 2 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Immunology 2023Quote: ... were isolated from the spleen and lymph nodes of FoxP3-aPIK3CD mice using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec). Genomic DNA was isolated from these cells using the AllPrep DNA/RNA Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Immunology 2023Quote: Splenocytes or small intestinal IELs isolated from QFLTg or OT-1 mice were depleted of non-T cells using Pan T isolation Kit II (Miltenyi Biotec). The negatively selected T cells were then labeled with 5µM Cell Tracing Violet (CTV ...
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bioRxiv - Genomics 2024Quote: All tumours were labelled with Annexin specific MACS beads using the Dead Cell Removal Kit (Miltenyi Biotec Cat# 130-090-101) following the manufacturers’ instructions and dead cells were removed by passing the labelled cells through the autoMACS® Pro (Miltenyi ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were stained with APC-eFluor780-conjugated anti-c-Kit (clone: 2B8) and fractionated using anti-APC magnetic beads and LS columns (both Miltenyi Biotec). c-Kit+ cells were then stained with combinations of antibodies as described in Supplemental Table 1 ...
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bioRxiv - Immunology 2024Quote: Single cells suspension from tumour-bearing lungs described previously were used for CD8+ T cells sorting using immunomagnetic sorting using CD8α+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells were isolated from peripheral blood mononuclear cells obtained from healthy donors using Human CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manual ...
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... OT-I CD8 T (OT-I) cells were enriched by negative selection using a CD8a+ T cell isolation kit (Miltenyi Biotec), commonly reaching a purity of 90-98% as determined by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the minced auricular lymph nodes using the CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: YFP+ Treg cells were isolated by sorting lymph node samples pre-enriched for CD25+ cells using a mouse CD25 Microbead kit (Miltenyi Biotec) on a FACSAria (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... was used to purify NK cells and the Human Naïve Pan T Cell Isolation kit (Miltenyi Biotec; CAT#130-097-095) was used to purify T cells ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... human CD3+ positive selection kit (# 130-050-101), and murine CD3+ and CD8+ negative selection kits (# 130-095-130, # 130-104-075) were purchased from Miltenyi Biotec. SiR-Actin (# CY-SC001 ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Cancer Biology 2024Quote: ... 3.5 µL 1 mg/mL Hoechst 33342 dye and 399 µL of permeabilization buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec).
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bioRxiv - Cell Biology 2024Quote: ... Autologous CD4+ T cells were isolated from PBMCs with a negative CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec).