Labshake search
Citations for Miltenyi Biotec :
1251 - 1300 of 1866 citations for RapidClean Protein Removal kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... the biopsies were cut into 2-3mm pieces and dissociated using a human Tumour Dissociation Kit (Miltenyi Biotec, Surrey, UK) according to the manufacturer’s protocol with some modifications ...
-
bioRxiv - Immunology 2021Quote: ... Allogenic naïve CD4+ T cells and CD8+ T cells were isolated from buffycoats using the naïve CD4+ T cell Isolation kit II (Miltenyi Biotec) and CD8+ T cell Isolation kit (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2020Quote: ... Single cell suspensions were generated using the Neural Tissue Dissociation Kit – Postnatal Neurons (Miltenyi Biotec. Cat no. 130-094-802), following manufacturer’s instructions for manual dissociation ...
-
bioRxiv - Neuroscience 2020Quote: ... Cell suspension was obtained by automated dissociation using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat#130-092-628) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2020Quote: EV preparation by immunobead isolation was performed using the Pan (mixture of CD9-, CD63- and CD81)-Exosome Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec). 2 ml of Amicon Ultra-15 (100 kDa cutoff ...
-
bioRxiv - Cancer Biology 2020Quote: ... polyA+ RNA was purified using the 96-well MultiMACS mRNA isolation kit on the MultiMACS 96 separator (Miltenyi Biotec, Germany) from total RNA with on column DNaseI-treatment as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... the brain tissues (cortices and hippocampi) were enzymatically dissociated with Neural Tissue Dissociation Kit-P (#130-092-628; Miltenyi Biotec). Myelin was removed by MACS Myelin Removal Beads II (#130-096-733 ...
-
bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... In vivo infections were performed by intranasal injection of 5×10^5 bacteria per mouse Lungs were collected after 20h from infection and digested using the Lung Dissociaton Kit according to the manufacturer’s instructions (Miltenyi Biotec) plus added Dispase II at 0,1U/ml (Roche) ...
-
bioRxiv - Cancer Biology 2020Quote: ... tumors (0.5 g) were mechanically disrupted by chopping and then chemically digested using Tumor Dissociation Kit (130-096-730, Miltenyi Biotec) and gentleMACS Dissociator (130-096-427 ...
-
bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells were purified via negative selection using the Naïve CD4+ T cell Isolation mouse Kit (Miltenyi Biotec) according to the manufacturer’s recommendations.
-
bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
-
bioRxiv - Molecular Biology 2022Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
-
bioRxiv - Immunology 2022Quote: ... total CD4+ T cells were directly isolated from buffy coats using the StraightFrom Buffy Coat CD4 MicroBead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
-
bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
-
bioRxiv - Immunology 2022Quote: Neutrophils were purified from freshly isolated mouse bone marrow cell suspensions using an autoMACS pro-selector cell separator with a MACS neutrophil isolation kit for negative selection in accordance with the manufacturer’s instructions (Miltenyi Biotec). Neutrophils were aged overnight in serum-free medium at 3 × 106 cells/ml at 37 °C and 5 % CO2 ...
-
bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
-
bioRxiv - Neuroscience 2020Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
-
bioRxiv - Microbiology 2020Quote: ... For the isolation of pDCs from PBMCs the Plasmacytoid Dendritic Cell Isolation Kit II from Miltenyi Biotec (# 130-097-415) was used ...
-
bioRxiv - Developmental Biology 2022Quote: ... spheres were dissociated using the MACS EB Dissociation Kit (order no. 130-096-348) according to manufacturer’s instruction (Miltenyi Biotec). The cell pellet was resuspended using Noci-3 medium (see Fig ...
-
bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were dissociated using TrypLE Express and enriched for CD34+ endothelial progenitor cells using the CD34 MicroBead Kit (Miltenyi Biotec). Purified cells resuspended in EGM-2 media (Lonza ...
-
bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
-
bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
-
bioRxiv - Immunology 2020Quote: ... T cells were isolated untouched using the magnetic human Pan-T-Cell Isolation Kit according to the manufacturer’s protocol (MACS, Miltenyi Biotec).
-
bioRxiv - Cancer Biology 2019Quote: ... Tumor tissues were mechanically and enzymatically dissociated using a human tumor dissociation kit (Miltenyi Biotec, Cat. No. 130-095-929), following the manufacturers recommendations ...
-
bioRxiv - Immunology 2019Quote: ... Pan T cells were isolated from peripheral blood leukocytes using the Pan T cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec). Isolated human T cells were cultured in X-VIVO-15 medium supplemented with 2 % human serum.
-
bioRxiv - Cell Biology 2020Quote: ... prior to separation into CD34+ and flowthrough (CD34−) cells using magnetic beads (CD34 Microbead Kit: Miltenyi Biotec, 130-046-702).
-
bioRxiv - Systems Biology 2021Quote: ... these mice were euthanized and the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (130-104-075, Miltenyi Biotec), resuspended in a concentration of 106/ml ...
-
bioRxiv - Microbiology 2021Quote: ... CD4+ T-cells were first isolated from HLA-DP4+ asymptomatic carriers using the Pan T-cell Isolation Kit (Miltenyi Biotec) and biotin-labeled anti-CD8 mAb (RPA-T8 ...
-
bioRxiv - Genomics 2021Quote: ... BMMNC were either cryopreserved in FBS with 10% of DMSO or previously enriched for CD34+ (CD34 MicroBead Kit, Miltenyi Biotec) before cryopreservation.
-
bioRxiv - Immunology 2021Quote: ... Naïve murine CD8+ T cells were isolated using negative selection (Naïve CD8 T Cell Isolation Kit, Cat no. 130-096-543, Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... we modified the Pan T cell negative Isolation magnetic-activated cell sorting (MACSR) kit (Miltenyi Biotec, Cat #130-096-535) by adding anti-CD45RA biotin (Miltenyi Biotec ...
-
bioRxiv - Immunology 2019Quote: CAR-and ΔLNGFR-positive T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Monocyte-derived dendritic cells (MDDCs) were generated from plastic adherent or directly isolated monocytes (Monocyte Isolation Kit II, Miltenyi Biotec) through culture for 6 days in Roswell Park Memorial Institute medium (RPMI-1640 ...
-
bioRxiv - Immunology 2019Quote: ... and T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Naïve antigen presenting cells were isolated from an unimmunized (naïve ...
-
bioRxiv - Immunology 2019Quote: ... WT mouse by depleting T cells from the spleen using the CD3ε microbead kit according to the manufacturer’s instructions (Miltenyi Biotec). T cells were stained with CellTrace Violet as described in the above section and plated in 96-well flat-bottom plates at 1.5×105 T cells per well with/without 2×104 antigen presenting cells isolated from naïve WT spleen ...
-
bioRxiv - Immunology 2021Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
-
bioRxiv - Immunology 2021Quote: ... OT-1 cells were purified from OT-1 transgenic mice using negative selection with CD8a+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec).
-
bioRxiv - Immunology 2021Quote: ... naïve splenic CD43− B cells were magnetically isolated using the B cell isolation kit (Miltenyi Biotec, Inc.; 130-090-862) and LS columns (Miltenyi Biotec ...
-
bioRxiv - Immunology 2021Quote: NK cell effectors were enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion Kit (Miltenyi Biotec). Target and effector cells were plated at E:T ratio of 2:1 in a 96 well round bottom plate ...
-
bioRxiv - Cell Biology 2020Quote: ... Lineage positive cells were depleted using a mouse lineage cell depletion kit (Miltenyi Biotec, 130-090-858, 130-110-470) and the following antibodies from BioLegend CD150 (Cat#115913 ...
-
bioRxiv - Developmental Biology 2021Quote: ... cells were washed with magnetic cell-sorting (MACS) buffer (PBS supplemented with 0.5% BSA and 5 mM EDTA) and incubated with CD117 (KIT) MicroBeads (Miltenyi Biotec) on ice for 20 min ...
-
bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
-
bioRxiv - Immunology 2021Quote: ... NK cells were isolated from spleen of P2Y6fl/fl and P2Y6ΔNcr1 mice fed a normal chow diet using the mouse NK cell isolation kit from Miltenyi Biotec, (Bergisch Gladbach ...
-
bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
-
bioRxiv - Immunology 2020Quote: ... Primary murine monocytes were isolated from C57BL/6J bone marrow using the Monocyte Isolation Kit (Miltenyi Biotec, 130-100-629) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...