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Citations for Miltenyi Biotec :
1201 - 1250 of 1745 citations for Mouse anti Plasmodium falciparum MSP1 PEM 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... They were activated by incubation with anti-biotin MACSiBead particles (Miltenyi Biotec) loaded with biotinylated anti-CD2 ...
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bioRxiv - Immunology 2021Quote: ... followed by positive immunomagnetic selection using anti-human CD34 microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and second with anti-CD11b MicroBeads (Miltenyi Biotec, Cat# 130-049-601) to collect the flowthrough fraction ...
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bioRxiv - Immunology 2021Quote: ... the isolated with anti-biotin Microbeads (Miltenyi Biotec, Cat# 130-090-485) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... The cells were incubated with purified anti-CD40 monoclonal antibody (Miltenyi Biotec) for 10 minutes before being stimulated (21) ...
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bioRxiv - Cell Biology 2022Quote: ... and then with anti-Rabbit IgG MicroBeads (Miltenyi Biotec, #130-048-602). The post-nuclear fractions were loaded on magnetic columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... CD45+ cells were positively selected using anti-CD45 TILs microbeads (Miltenyi Biotec). The frequency of Tregs infiltrating in the tumor microenvironment was defined as FOXP3+CD25+CD4+ among CD45+ live cells and assessed by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... and monocytes were then enriched using anti-Human CD14 beads (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Anti-biotin-PE antibody was purchased from Miltenyi Biotec (130-113-291) for biotin–LPETG SrtA substrate staining ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended in the presence of anti-biotin MicroBeads (1:5; Miltenyi Biotec) and incubated for 15 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... followed by positive immunomagnetic selection with anti-human CD34 microbeads (Miltenyi Biotec). Purity (>90% CD34+ cells ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were first labeled with PE-conjugated anti-CD154 (5C8, Miltenyi Biotec) and CD154+ cells were then enriched using anti-PE magnetic beads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... or anti-PE MultiSort microbeads as per the manufacturer’s instructions (Miltenyi Biotec). Purity was assessed by flow staining against knock-in markers tEGFR and tNGFR ...
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bioRxiv - Physiology 2023Quote: ... The cell suspension was incubated with anti-CD31 MicroBeads (Miltenyi Biotec, Germany) to pre-select PECAM-1high cells using MACS LS columns and a magnetic separator ...
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bioRxiv - Immunology 2024Quote: ... and human Treg expansion kit anti-CD3/CD28 beads (Miltenyi Biotec/Invitrogen), using x-vivo 15 cell medium (Lonza ...
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bioRxiv - Immunology 2022Quote: ... anti-CD158b (KIR2DL2/DL3) APC vio 770 (clone REA 1006, Miltenyi Biotec), CD85j (ILT2 or CD94 ...
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bioRxiv - Immunology 2023Quote: ... washed and incubated with anti-PE magnetic beads (Miltenyi Biotec, Cologne, Germany) followed by magnetic separation using an AutoMACSpro (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... SiglecF-positive cells were enriched using anti-PE MultiSort Kit (Miltenyi Biotec). 1x105 flow through and AM enriched fractions were co-cultured with 1x105 hCD1Tg BMDCs and 3x105 DN1 T cells for 48 hours and cells were stained for the expression of activation markers.
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bioRxiv - Genetics 2023Quote: ... and incubated with anti-SSEA1 micro beads (Miltenyi Biotec, 130-094-530) at 4°C for 15 minutes ...
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bioRxiv - Genomics 2023Quote: SUM159PT_KRAB cells were stained with anti-TM4SF1-APC (clone: REA851 Miltenyi Biotec) antibody for 10’ at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked with 0.5μg/ml of anti-CD40 mAb (Miltenyi Biotec) for 15min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... combined with an anti-PE or -AF647 magnetic separation technique (Miltenyi Biotec) to enrich for CCR8+ iTregs ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by washing and incubation with anti-FITC MACS microbeads (Miltenyi Biotec) for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... followed by positive immunomagnetic selection using anti-human CD34 microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: Anti-CD11c-APCvio770 (2µl/sample, cat. no.: 130-113-585, Miltenyi Biotec)
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bioRxiv - Developmental Biology 2020Quote: ... cell suspension with PBS supplemented with 0.5% BSA and 2 mM EDTA was applied to an MS column (Miltenyi Biotec, 130-042-201). After negative cells flow through the column ...
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bioRxiv - Microbiology 2023Quote: Approximately half of the lung and the left hemisphere of each brain were weighed and homogenized in 1 mL of DMEM supplemented with 2% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Neuroscience 2023Quote: ... this tissue was transitioned to a 2 ml Dounce homogenizer containing 1.5 ml of nuclei extraction buffer (130-128-024, Miltenyi Biotec, Bergisch Gladbach, Germany) along with 0.2 U/ml Ribolock RNase inhibitor ...
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bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were isolated from mouse lung tissue (without the trachea) using the QuadroMACS™ Separator and antibody-bound magnetic beads (Miltenyi Biotec, Leiden, The Netherlands). EpCAM+ cells and fibroblasts were mixed 1:1 (20,000 cells each ...
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bioRxiv - Immunology 2023Quote: Spleens from four- to six-week-old mice were processed to single cell suspensions and CD4+ T cells were enriched using mouse CD4 (L3T4) Microbeads kit (Miltenyi Biotec Cat # 130-117-043) according to manufacturer instructions ...
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bioRxiv - Physiology 2023Quote: ... Hearts were cut in small pieces and dissociated at 37°C with the commercially available enzyme mix (Neonatal Heart Dissociation Kit, mouse and rat, Miltenyi Biotec GmbH; # 130-098-373) followed by tissue homogenization with the genteMACS™ Dissociator (Miltenyi Biotec GmbH ...
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bioRxiv - Cell Biology 2023Quote: Murine nCMs were isolated from the ventricles of 1-2 days old wildtype C57BL/6 and Lmnaflox/flox mouse pups using MACS neonatal heart dissociation kit according to the manufacturer (Miltenyi Biotec cat# 130-098-373). Following digestion ...
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bioRxiv - Bioengineering 2022Quote: ... and lungs were processed into single-cell suspensions with a gentle MACS™ Octo Dissociator and Mouse Lung Dissociation Kit (Miltenyi Biotec, #130-095-297) according to manufacturer protocols.
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with anti-human CD8-FITC (BW135/80, Miltenyi biotec) and anti-human CD4-PE-Cy7 (SK3 ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were conjugated to anti-PE microbeads (130-048-801; Miltenyi Biotec) and passed through 3 LS Columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was incubated with 50 mL magnetic µMACS Anti-DYKDDDDK MicroBeads (Miltenyi Biotec) for 30 minutes on ice ...