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Citations for Miltenyi Biotec :
1151 - 1200 of 1703 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: MDSCs were isolated from the spleens of 4T1 tumor-bearing mice using a Mouse MDSC Isolation Kit (Miltenyi Biotec, Cat# 130-094-538) and plated in RPMI1640 supplemented with 10% FBS and antibiotics ...
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bioRxiv - Immunology 2024Quote: ... Primary monocytes and neutrophils were isolated from mouse bone marrow using negative isolation kits (Miltenyi Biotec, 130-100-629 and 130-097-658). Cells were cultured in RPMI 1640 media (monocytes ...
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bioRxiv - Immunology 2024Quote: ... naïve CD8+ T cells were purified by magnetic bead separation using mouse naïve CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-096-543) following vendor instructions ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched for using the Miltenyi Mouse Naïve CD4+ T cell Purification Kit (Miltenyi Biotec, Cat. # 130-104-453) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Tumours were dissected at endpoint and were processed into single-cell suspensions using a Mouse Tumour Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Monocytes were isolated from freshly collected PBMCs using CD14 antibody-conjugated microbeads and magnetic columns (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and positively selected using 10 uL PDGFRα antibody-coated microbeads (1:10; Miltenyi Biotec #130-101-547). PDGFRα+ stoma was placed into magnetic separation MS columns (Miltenyi Biotec #130-042-021 ...
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bioRxiv - Cell Biology 2020Quote: ... and primary antibodies against CD45-FITC (12μl/sample, Cat nb. 130-114-567, Clone 5B1, Miltenyi Biotec), CD31-FITC (4μl/sample ...
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bioRxiv - Bioengineering 2020Quote: ... CD3 and CD28 monoclonal antibodies (beads T cell activation/Expansion Kit, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) were co-cultured with WJ-MSCs (A/ WJ hMSCs ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies were diluted with Running Buffer in a MACSwell™ Deepwell Plates (Miltenyi Biotec, 130-126-865) in a total volume of 1050 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against CD14 (#130-110-583) and HLA-DR (#130-111-943) were obtained from Miltenyi Biotec. Dapi (#422801 ...
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bioRxiv - Cancer Biology 2024Quote: ... immunomagnetic microbeads decorated with recombinantly engineered antibody fragments for CD11b (Miltenyi Biotec, Cat. No. 130-049-601) were used with the MiniMACS Separation Unit (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2023Quote: ... µMACS DYKDDDK Isolation Kits were used for anti-FLAG IPs and HA Isolation Kits for anti-HA immunopurification (Miltenyi Biotec #130-101-591 and #130-091-122). Next ...
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bioRxiv - Immunology 2024Quote: ... 0.5% BSA in PBS) and then incubated with 20 μl of anti-APC and anti-PE microbeads (Miltenyi Biotec, Cat #: 130-090-855 and 130-048-801) in 100 μl of MACS buffer at 4°C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Neuroscience 2021Quote: ... and the brains were extracted and dissociated using Miltenyi Adult mouse and rat Brain Dissociation Kit and the gentleMACS Octo Dissociator (Miltenyi Biotec 130-107-677). The cells were then resuspended in PBS + 0.5% BSA and stained with DAPI to determine viability ...
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bioRxiv - Immunology 2022Quote: Lungs were isolated and processed into single cell suspension using gentleMACS tissue dissociator and mouse lung dissociation kit (Miltenyi Biotec Ref. 130-095-927) followed by 40 μm filtration and red blood cells lysis with ACK lysis buffer ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were purified from CD4+ T cells from pooled spleens and lymph nodes of Cd226−/− 2D2 TCR Tg mice by using mouse CD4 MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany), followed by flow cytometric sorting for CD25-CD44LoCD62LHiCD4+ cells ...
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bioRxiv - Bioengineering 2022Quote: ... ≈1 gram of muscle tissues was dissected from the hindlimb muscles of a humanely euthanized mouse and placed into a GentleMACS dissociation tube (Miltenyi Biotec, #130-096-334). 7 mL of DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: Total spleen T cells were purified by negative selection with the mouse naïve CD4+ T Cells Isolation Kit (Miltenyi Biotec, Cat# 130-104-453) and LS separation columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in fresh media containing either 100 nM CCK or 100 nM saline vehicle control up to 72 hours with concurrent treatment with a mouse cytokine cocktail containing 50-ng/mL TNFα (Miltenyi Biotec, #139-101-687), 10-ng/mL IL-1β (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
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bioRxiv - Molecular Biology 2023Quote: B cells were purified from red blood cell-lysed single-cell suspensions of mouse spleens by magnetic negative selection using a B Cell Isolation Kit (Miltenyi Biotec, 130-090-862). B cells (3x105 per well in a 12-well plate ...
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bioRxiv - Molecular Biology 2024Quote: ... Lin- cells isolated from the bone marrow of donor mice (8–12 weeks old) via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA, USA). Lin- cells were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Immunology 2023Quote: ... cells were spun down at 500g for 10 minutes and resuspended in MACS buffer to purify PMNs with a magnetic purification kit according to manufacturer’s instruction (Miltenyi Biotec; Neutrophil Isolation Kit, Mouse).
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bioRxiv - Cancer Biology 2022Quote: ... and mechanically and chemically digested into single-cell suspensions using the gentleMACS Dissociator and the mouse tumor dissociation kit by Miltenyi Biotec (130-096-730) respectively ...
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bioRxiv - Cancer Biology 2022Quote: Thyroid lobes (8 lobes/condition/experiment) were cut in 1mm3 pieces and processed into single cells suspensions with the Mouse Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730) diluted in Ca2+ free DMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched from bulk tumor cell suspensions using CD45 (TIL) mouse Microbeads kit followed by magnetic separation on LS columns (Miltenyi Biotec, Bergisch-Gladbach, Germany). After eluting CD45+ cells from the column ...
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bioRxiv - Immunology 2024Quote: ... CD4 4+ T cells and total T cells were purified from cell suspensions using Mouse CD4+ T cell and total T cell Isolation Kits from Miltenyi Biotec (Auburn, CA, USA) (Supplemental material) ...
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bioRxiv - Bioengineering 2024Quote: Harvested brain tissue was converted to single cell suspensions using the papain-based mouse adult brain dissociation kit from Miltenyi Biotec (130-107-677), with appropriate steps for myelin removal and red blood cell lysis according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were sacrificed at predetermined timepoints and human CD45+ cells were isolated from bone marrow and spleen using the mouse cell depletion kit (Miltenyi Biotec, #130-104-694) to achieve >90% purity of human cells.
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bioRxiv - Developmental Biology 2024Quote: ... forelimb and hindlimb muscles from P4 Poglut1-cKO and sibling controls were dissociated using the mouse Skeletal Muscle Dissociation Kit and the gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were suspended in a 0.5% NGS solution then incubated with anti-PDGFRα or anti-O4 coupled-beads (Miltenyi Biotec; ref 130-094-543 and 130-096-670). Unbound bead-coupled antibodies were washed away by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with Anti-TRA-1-60 MicroBeads (Miltenyi Biotec, Germany) before negative sorting with an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec), then stained with the I-A/E-APC antibody (MHCII ...
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bioRxiv - Neuroscience 2020Quote: ... with LS columns and Anti-PE MicroBeads (Miltenyi Biotec 130-048-801) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Cells were activated with soluble human anti-CD3 (clone OKT3, Miltenyi Biotec) and human anti-CD28 (clone 15E8 ...
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bioRxiv - Immunology 2021Quote: ... cell suspensions were counted and stained with anti-CD45 microbeads (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: Human and mouse DCs were stained with the following antibodies from Miltenyi Biotec or as specified: Vioblue-conjugated anti-human CD86 (clone FM95, Miltenyi Biotec), PE-conjugated anti-human CD83 (clone REA714) ...
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bioRxiv - Immunology 2021Quote: ... and stained with APC-conjugated anti-Granzyme B (clone REA226, Miltenyi Biotec). Cell viability was assessed by LIVE/DEAD staining according to the manufacturer’s instruction (Molecular Probes ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-MHC Class II (Miltenyi Biotec, 130-102-139, clone M5/114.15.2), anti-ICAM-1 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were purified using anti-CD4+ conjugated microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with anti-O4 Microbeads (130-094-543, Miltenyi Biotec) and magnetic separation was done using Multi-24 Column Blocks and the MultiMACS Cell24 Separator Plus (130-095-692 and130-098-637 ...
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bioRxiv - Physiology 2021Quote: ... PBMCs purification was performed using anti-human CD14 magnetic beads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4C with anti-human Fc Block (Miltenyi Biotec). Cells were incubated with DAPI viability dye (Thermo ...