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Citations for Miltenyi Biotec :
1101 - 1150 of 2984 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells were isolated by ficoll density gradient centrifugation (Dakewe) and T cells were purified using the Pan T Cell Isolation Kit (Miltenyi Biotec), and stimulated with CD3/CD28 T cell Activator Dynabeads (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... autologous T cells were isolated from patient NSCLC lesions or from healthy donor PBMCs using the Pan T-cell Isolation Kit (Miltenyi Biotec). Autologous T cells were co-cultured with either ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... of cytapheresis rings from healthy donors (EFS Bordeaux) and B cells were sorted with the B cell isolation kit II (Miltenyi Biotec). Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... through the New York Blood Center and CD4+ cells isolated by negative selection with a Miltenyi CD4 T cell isolation kit II (Miltenyi Biotec). Cell-free virus was produced by transfection of 293T cells in 10 cm plates using polyjet (Signagen) ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were collected from spleens and lymph nodes of naïve CD45.1+ and CD45.1.2+ P14 TCR transgenic mice and negatively enriched for CD8+ T cells using the CD8a+ T Cell Isolation Kit and LS MACS Columns (Miltenyi Biotec). 1×106 CD8+ T cells were plated per well in 48 well flat-bottom plates ...
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bioRxiv - Immunology 2020Quote: T cells were isolated from splenocytes of 8-12-week-old mice using a Pan T Cell Isolation Kit II (Miltenyi Biotec) with >90% purity ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed and CD11c+ dendritic cells were enriched using CD11c MicroBeads UltraPure (mouse) (Miltenyi Biotec, #130-125-835).
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bioRxiv - Immunology 2022Quote: ... The cell concentration and viability were determined using an automatic cell counter (Cellometer Auto 2000, Nexcelom Bioscience, Lawrence, MA, USA) and the Dead Cell Removal Kit (Miltenyi Biotec) was used to remove dead cells to obtain a cell viability of ≥80%.
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bioRxiv - Immunology 2022Quote: PCLS were dissociated as described above and dead cells were removed using Miltenyi Dead Cell Removal Kit (130-090-101, Miltenyi Biotec). For the multiplexing purpose ...
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bioRxiv - Immunology 2022Quote: ... We then purified CD4 CD25 and CD4+CD25− T cells using CD4+CD25+ Regulatory T Cell Isolation Kit (#130-091-301) and AutoMACS technology (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were enriched from PBMCs and tissue mononuclear cells using a negative selection magnetic-activated cell sorting (MACS) system as per manufacturer’s instructions (Miltenyi Biotec, Germany). Enriched CD4+ T-cells were stained with fluorescently conjugated antibodies listed in Table S2 ...
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bioRxiv - Immunology 2019Quote: ... cells were washed and lineage-negative precursor cells were sorted by negative selection using magnetic beads (130-110-470, Miltenyi Biotec). Lineage-negative cells were subsequently propagated analogous to the protocol described by Dahlin et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... The NCAM+ Merkel cells in our cell suspension were bound by anti-PSA-NCAM microbeads (Miltenyi Biotec, Cat. 130-097-859) and extracted with the MACS® Column system ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from PBMCs by a magnetic bead “negative” isolation procedure using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and LS columns (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were harvested with TrypLE express, and CD34+ cells were isolated by MACS (CD34 microbeads, (#130-046-703 Miltenyi Biotec). Cytokines were purchased from R&D otherwise indicated.
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bioRxiv - Immunology 2021Quote: B cells were isolated from spleens of WT female mice and enriched with a B cell isolation kit (Miltenyi Biotec MACS). The purity of isolated B cells was 96±0.8% ...
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bioRxiv - Immunology 2020Quote: ... CD137-expressing CD4+ memory T cells cells were positively selected by staining with biotin-conjugated CD137 antibody (clone REA765; Miltenyi Biotec) followed by anti-biotin MicroBeads and applied to a new MS column ...
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bioRxiv - Immunology 2021Quote: ... Conventional T cells were enriched from FVB/N mouse spleen single-cell suspensions using the mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2021Quote: ... B cell isolation from cryopreserved PBMCs was carried out using Memory B cells Isolation Kit (MACS/Miltenyi Biotec, Bergisch Gladbach, Germany). Next ...
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
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bioRxiv - Immunology 2020Quote: ... PolyPeptide group) were co-cultured with 105 OTII CD4+ T cells (purified with a CD4+ T cell isolation kit, Miltenyi Biotec) in RPMI medium (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using magnet activated cell sorting (MACS) using the Pan T cell Isolation Kit (negative selection) according to the manufacturer’s instructions (Miltenyi Biotec, Germany). Following separation ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions obtained from spleens were processed with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec; Cat#: 130-104-075) and transferred via the tail vein into recipient mice.
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bioRxiv - Cancer Biology 2022Quote: ... After the centrifugation they were resuspended in MACS buffer and the magnetic separation was performed using LS MACS column (maximum 108 labeled cells) for positive selection and LD MACS column (maximum 108 labeled cells) for negative selection (Miltenyi Biotec). The purity of positive subpopulation was >70% and <99% for the negative subpopulation.
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bioRxiv - Bioengineering 2022Quote: ... mouse bone marrow neutrophils were sorted from the bone marrow cell suspension using a mouse neutrophil isolation kit that enriches for CD11b+Ly6G+ cells (Miltenyi Biotec; negative selection using a cocktail that contains anti-CD5 ...
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bioRxiv - Immunology 2022Quote: ... OT-1 T cells were isolated from the spleens OT-1 mice using negative selection with CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from peripheral blood lymphocytes by negative selection using a CD4 T-cell isolation kit II and magnetic cell separation (Miltenyi Biotec). A total of 50,000 CD4+ T cells were cultured in IMDM (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... ratio were purified by magnetic-activated cell sorting (MACS®) according to the manufacturer’s instructions (NK cell Isolation kit; Miltenyi Biotec). Total RNA from the samples was isolated using TRIzol® reagent following the manufacturer’s isolation protocol (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: Previously cryopreserved PBMCs were thawed and CD8+ T cells were enriched by negative selection using a nonhuman primate CD8+ T cell isolation kit (Miltenyi Biotec). RNA was isolated from the enriched CD8+ T cells by TRIzol/phenol chloroform extraction with DNase treatment ...
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bioRxiv - Immunology 2022Quote: ... 0.1 mg ml−1 DNAase I in RPMI-1640 medium and enriched by depleting CD45+ cell and TER119+ cells using MACS microbeads (Miltenyi Biotec). Cells suspensions were incubated with anti-CD45 (30-F11 ...
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bioRxiv - Cell Biology 2023Quote: ... Three independent replicates of Epcam-positive kidney epithelial cells (control kidney, CK) were MAC-sorted from freshly isolated single cell suspensions from whole mouse kidneys using microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: PBMCs from three different donors were isolated from human buffy coat by Ficoll density gradient centrifugation and untouched B-cells were purified by negative magnetic enrichment using a B-cell isolation kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... CD11b/c positive cells (monocyte/macrophage) were sorted using magnetic-activated cell sorting (MACS) separation columns (Miltenyi Biotec, 130-105-634) and immediately lysed with TRIzol (Fischer Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from wildtype mice spleens using CD8a+ T Cell Isolation Kit (130-104-075) from Miltenyi Biotec according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: NK cells were enriched from the spleens of C57BL/6 Kaede mice using NK Cell Isolation Kit (#130-115- 818, Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: Resting B-cells were enriched from the spleen using the B-cell Isolation Kit (Miltenyi Biotec, Germany, Cat# 130-090-862). 1 mL 8 million/mL B-cells in HBSS buffer were sequentially incubated with 1 μM cholesterol strand (30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... CD34+ cells were purified from a portion of the cells using the CD34 Microbead Kit Ultrapure and LS columns (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Immunology 2023Quote: ... Cells (5×106) were infected at 5000 TCID50 / 1×106 cells at 37 °C for 2 hours on a cell-rotator (Miltenyi Biotec). After infection ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...