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Citations for Miltenyi Biotec :
1051 - 1100 of 1279 citations for Anti CKAP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... followed by 30 min staining at room temperature with PE-conjugated anti-Flag (clone REA216, Miltenyi Biotec) or pan-HLA class I-specific antibodies (clone W6/32 ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... with the exception that dissociated β-like cells were directly incubated with anti-CD19 MicroBeads (Miltenyi Biotec) instead of a primary antibody.
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bioRxiv - Neuroscience 2023Quote: ... CD11b+ cells were isolated by anti-CD11b MicroBeads (cat # 130-093-634, MACS Miltenyi Biotec, Auburn, CA). CD11b+ cells-removed cell pellets were resuspended and incubated with FcR blocking reagent for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE-Vio® 770 anti-human CD14 (clone TÜK4, dilution 1/50, Miltenyi Biotec, Paris, France) antibodies were used for macrophages phenotyping ...
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bioRxiv - Immunology 2023Quote: ... anti-FITC microbeads (130-148-701) and LS Columns (130-042-401) were purchased from Miltenyi Biotec. FITC Mouse Anti-Human CD4 (555346 ...
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bioRxiv - Immunology 2022Quote: ... and the pellet resuspended prior to the addition of 8.33 μL of anti-APC microbeads (Miltenyi Biotec). Following a 30 minute incubation on ice ...
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bioRxiv - Immunology 2024Quote: ... and subsequently with anti-FITC microbeads and enriched by magnetic isolation using LS MACS columns (Miltenyi Biotec). After CD137 enrichment ...
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bioRxiv - Neuroscience 2024Quote: ... dissociated cells were incubated with biotin conjugated anti-human CD171(L1CAM) (Miltenyi Biotec, Cat#130-124-046) in MACS buffer at 4°C for 10 min ...
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bioRxiv - Immunology 2024Quote: ... and then PE+ CD3+ T cells were enriched using anti-PE Microbeads with LS columns (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... also known as LPEC-1 and -6 in previous studies) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) 10-13 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human liver parenchymal primary ECs (EC9) lines were isolated and established in our laboratory using MACS microbead-conjugated antibodies and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany). ant-CD31 was used for sorting of ECs ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Genomics 2022Quote: ... Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... 2mM ethylenediaminetetracetic acid (EDTA) and PBS and incubated with either CD133-APC or CD133-VioBright FITC antibodies (both monoclonal mouse, Miltenyi Biotec, dilution 1:500) and FcR blocking reagent (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... single cells were obtained as described for flow cytometry from femurs and tibias of 12-week-old male mice and stained with microbead labelled antibodies to CD45 (Miltenyi Biotec, 130-052-301), CD117 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...
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bioRxiv - Physiology 2023Quote: ... the cells were mixed with CD45 antibody-coated magnetic beads (CD45 MicroBeads, 130-052-301) and then passed through a MACS LD column (Miltenyi Biotec, 130-042-901) on a MidiMACSTM separator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the PMN suspension was analyzed by flow cytometry (CD3-PerCP-/CD66b-FITC+/CD14-PElow cells > 90%, antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Microbiology 2023Quote: ... Cells were stained extracellularly with fluorescent antibodies listed in Supplementary Table S2 and the fixable ViobilityTM Live/Dead Dye (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation in the dark for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... the flow through eluent from microglia selection served either as a cell type fraction depleted of microglia (denoted as “flow through”) or was used for an additional separation between neuronal and glial fractions by using PSA-NCAM antibody (Miltenyi Biotec, 130-092-966) following the same procedure described for microglia purification.
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bioRxiv - Cancer Biology 2023Quote: CD40 stimulation medium were prepared by incubation of 40 uL of CD40 ligand and 40 uL of cross-linking antibody (Miltenyi Biotec 130-106-196) mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196) ...
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bioRxiv - Cell Biology 2020Quote: The isolation of Thy1 positive EVs is performed by positive selection using anti-CD90 MicroBeads (Miltenyi Biotec, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were purified from mouse bone marrow using anti-Ly6G MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2020Quote: ... AnnexinV-FITC, PI (MBL, code: 4700) and ANTI-PSA-NCAM conjugated-Ab (Miltenyi Biotec, cat. 130-093-273) were added according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... CD14+ cells were positively selected from PBMC using anti-CD14-coated magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Then cells were cultured in 24-well plates (5×105 cells/mL) ...
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bioRxiv - Cancer Biology 2020Quote: ... After washing with cold MACS buffer stained cells were incubated with anti-biotin-microbeads (Miltenyi Biotec, Auburn, CA) for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Bioengineering 2021Quote: ... CD14+ monocytes were collected in the resuspended cell suspension by incubating with anti-human CD14 microbeads (Miltenyi Biotec) for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2021Quote: ... EpSCs were isolated using magnetic-associated cell sorting using Anti-Sca-1 microbeads (Miltenyi Biotec, 130-106-641) and a MACS MultiStand system (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
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bioRxiv - Immunology 2021Quote: ... Naïve mature B cells were isolated from spleen cells by immunomagnetic negative selection with anti-CD43 (Miltenyi Biotec). B cells were cultured in RPMI-1640 medium (Corning ...
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bioRxiv - Cancer Biology 2020Quote: For CSR experiments spleen naive B cells were isolated by immunomagnetic depletion using anti-CD43 beads (Miltenyi Biotec). Purified naïve B cells were cultured at 1.2 x 106 cells/ml in complete RPMI medium supplemented with 10% FBS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). Purity checks and cell counts were performed using a BD Accuri C6 flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... and stained with 100 μl PE-conjugated mouse anti-human IgA (1:40; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Immunology 2022Quote: Monocyte isolation was carried out by using Anti-CD14+ Micro Beads (catalog 130-050-201, Miltenyi Biotec, Germany) from PBMCs separated from the blood samples ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from the spleen and peripheral lymph nodes using anti-CD4 microbeads (Miltenyi Biotec) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Physiology 2022Quote: ... samples were resuspended and incubated with 80 μL anti-PE microbeads per 107 cells (Miltenyi Biotec, Cologne, Germany) for 20 minutes at 4°C before Auto MACS cell separation (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked at 37 C for 15 min with 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec). A stimulation with an equimolar amount of DMSO was performed as negative control ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were blocked at 37°C for 15 min with 0.5 μg/ml anti-CD40 mAb (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2019Quote: ... followed by separation with anti-PE magnetic beads using MS columns (both from Miltenyi Biotec, Bergisch Gladbach, Germany). THP1 monocytic cells were maintained in R10.
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bioRxiv - Developmental Biology 2020Quote: ... resuspended pellets to single cells and incubated with Anti-SSEA-1 (CD15) Microbeads (Miltenyi Biotec, 130-094-530). After collected the positive cells ...
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bioRxiv - Cancer Biology 2020Quote: Cells were stained with anti-human CD34 (AC136, APC, Miltenyi Biotec, Cat No 130-113-738, 1:50) and anti-human CD38 (IB6 ...
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bioRxiv - Immunology 2020Quote: ... CD45+ hematopoietic cells were depleted using anti-CD45 magnetic beads by autoMACS with the depleteS program (Miltenyi Biotec). Total mTECs (EpCAM+UEA-1+Ly51lo) ...
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bioRxiv - Immunology 2021Quote: ... TCRβ+CD138- and TCR+CD138+ cells were further separated with anti-PE magnetic microbeads (Miltenyi Biotec, Auburn, CA). Depending on the experimental objective ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Cell samples were fluorescently stained with Anti-ACSA-2-PE (clone: IH3-18A3) (130-102-365, Miltenyi Biotec), examined by a BD FACSCanto II flow cytometer ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and labeled with CD154-Biotin followed by anti-Biotin MicroBeads (CD154 MicroBead Kit; Miltenyi Biotec) and magnetically enriched by two sequential MS columns (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2024Quote: ... and the brain homogenate pellet was incubated separately with Anti-CD11b Microbeads (130-093-634, Miltenyi Biotec, Germany) and Anti-O4 Microbeads (130-094-543 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...