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Citations for Miltenyi Biotec :
1001 - 1050 of 1749 citations for Protein Dialysis kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD8+ T cells were purified from the spleen of OT-I.Ly5.1 mice using CD8a+ T Cell Isolation Kit II (Miltenyi Biotec). OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2020Quote: Neonatal primary cardiac cultures were isolated from P0 pups with a neonatal dissociation kit (Miltenyi Biotec, 130-098-373) and homogenizer (gentleMACS) ...
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bioRxiv - Molecular Biology 2021Quote: ... endothelial (CD31+) and myeloid (CD45+) cells were sorted using the Magnetic Cell Isolation and Cell Separation kit (Miltenyi Biotec) with recommended dilutions of antibodies against CD31 (Miltenyi Biotec 130-097-418 ...
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bioRxiv - Cell Biology 2022Quote: ... and mitochondria were isolated and enriched using a Mitochondria isolation kit as the manufacturer’s introductions (Cat#130096946, Miltenyi Biotec). HeLa cells were harvested and permeabilized by 0.025% w/v digitonin (Biosynth ...
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bioRxiv - Immunology 2022Quote: CD8 T cells were purified from PBMC by negative immunomagentic sorting using CD8+ T Cell Isolation Kit (Miltenyi Biotec) and used for bilayers experiments or for further separation into the subsets ...
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bioRxiv - Immunology 2022Quote: ... Mouse hepatic parenchymal cells were digested and non-parenchymal cells were isolated by Mouse Liver Dissociation Kit (Miltenyi Biotec). Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Microglia were isolated from adult mouse brain using the multi tissue dissociation kit 1 (Miltenyi Biotec #130-110-201), followed by CD11b microglia isolation kit (Miltenyi Biotec #130-093-634 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse CAFs were separated from human pancreatic UM2 cancer cells using the Mouse Cell Depletion Kit (MACS Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2021Quote: ... Tumor specimen was dissociated with the use of a papain-based tumor dissociation kit (Miltenyi Biotec, 130-095-942) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and CD11b+ CD11c+ cells were isolated from adult murine retinas using the Miltenyi Adult Brain Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...
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bioRxiv - Immunology 2021Quote: Primary murine naïve CD4 T cells were isolated from the spleens and lymph nodes of SLE1.2.3 or control mice using the CD4+CD62L+ T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). CD62L positive selection was routinely ∼98% in purity as determined by flow cytometry ...
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bioRxiv - Molecular Biology 2022Quote: EV samples were tested with a bead-based multiplex analysis (MACSPlex exosome kit, human, Miltenyi Biotec, Bergisch Galdbach, Germany). EV samples were diluted with MACSPlex buffer to a final volume of 120 µl with 5.8×108 EVs per sample ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens by using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and the tissue was enzymatically dissociated using the Neural Tissue Dissociation Kit (Miltenyi Biotec, Germany, cat# 130-092-628) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: Tumors and 1 cm2 of jejunum were cut into small pieces using the McIlwain™ Tissue Chopper and digested on the gentleMACS™ Octo Dissociator with Heaters (program, 37C_m_TDK_1) using the mouse Tumor Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor and matched normal samples were dissociated into single cell suspension using human tumor dissociation kit (Miltenyi Biotec) and gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Neuroscience 2022Quote: ... We used our previous established isolation protocol37 using gentleMACS™ with the Neural Tissue Dissociation Kit (Papain) (Miltenyi Biotec) and a final concentration of 45 mM actinomycin D (Act-D ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: CD34+ haematopoietic progenitor cells (HPC) were isolated by magnetic-activated cell sorting using the CD34 MicroBead kit (Miltenyi Biotec) and cultured for 16 hours in preculture medium ...
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bioRxiv - Immunology 2022Quote: CD4 T cells were enriched from PBMCs by negative immunomagnetic selection using CD4+ T cell isolation kit (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed from the digested PDX using a Dead Cell Removal Kit (130-090-101, Miltenyi Biotec). Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694 ...
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bioRxiv - Molecular Biology 2019Quote: Mitochondria were isolated from WT and NSUN2 KO HEK293T cells (1 × 107) using the Mitochondria Isolation Kit (Miltenyi Biotec). Steady-state levels of subunit proteins of mitochondrial respiratory chain complexes were analyzed by immunoblotting with Total OXPHOS Rodent WB Antibody Cocktail (1:250 ...
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bioRxiv - Immunology 2019Quote: Human neutrophils were isolated by magnetic separation using MACSxpress whole blood neutrophil isolation kit (Miltenyi Biotec, 130-104-434) and were seeded into a micro-insert 4 well dish (Ibidi 80406) ...
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Germany), resuspended at 106 cells/ml ...
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bioRxiv - Physiology 2021Quote: ... Satellite cells were purified from this cell suspension using magnetic bead separation (Satellite Cell separation kit from Miltenyi Biotec). For protein and gene expression assays of freshly isolated cells ...
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bioRxiv - Immunology 2019Quote: ... An antibody cocktail designed for negative selection of purified naïve T cells (Naïve Pan T cell purification kit, Miltenyi Biotec) was used together with extra αCD15/CD14 beads ...
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bioRxiv - Cell Biology 2021Quote: T lymphocytes were isolated from healthy donor’s PBMCs via Pan T Cell Isolation Kit (Miltenyi Biotec,130-096-535) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were digested using the Neural Tissue Digest Kit-P in C-tubes as per manufactures directions (Miltenyi Biotec). Two brains were digested in each C-tube using program 37_ABDK on the GentleMACS™ OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... The remaining tissue was further minced with scissors and enzymatically dissociated using the Human Tumour Dissociation Kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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bioRxiv - Immunology 2021Quote: ... Cells were negatively enriched for CD8 cells using the Miltenyi Biotec mouse CD8+ T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Genomics 2020Quote: ... The dissected cortex was dissociated using a papain-based neural dissociation kit (Miltenyi Biotec, Cat. N: 130-092-628) according to the manufacturer protocol with minor modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... The naïve T cells were purified from human PBMC using Pan T cell isolation kit (Miltenyi Biotec, Cat#130096535) and activated and expanded by CD3/CD28 Dynabeads (GibcoTM ...
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bioRxiv - Immunology 2020Quote: Neutrophils from bone marrow were purified from C57Bl/6 mice by negative selection using neutrophil isolation kit (Miltenyi Biotec). Purified neutrophils were cultured at 1.0-5.0 x 107 total cells and were infected or not at different ratios with RFP LmRyn metacyclic promastigotes ...
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bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ml of Enzyme R and 25 ml of Enzyme A (Miltenyi Biotec, Tumor Dissociation Kit 130-095-929). Tumor samples were dissociated using the gentleMACS h_tumor_01 program for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: Mature mouse and human retinal organoids were dissociated using the embryoid body dissociation kit (Miltenyi Biotec, 130-096-348) with the gentleMACS™ Dissociator according to the manufacturer’s automated dissociation protocol with a minor modification ...
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bioRxiv - Immunology 2022Quote: ... The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit, Miltenyi Biotec). Antibody-loaded anti-biotin MACSiBead™ were added to the TIL at a cell/bead ratio of 1:1 ...
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bioRxiv - Genomics 2022Quote: ... and processed into single-cell suspensions using the Whole Skin Dissociation Kit (Miltenyi Biotec, Cat No.130-101-540) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2022Quote: CD3+/CD4+ T cells were purified by positive selection using magnetic separation with a CD4+isolation kit (Miltenyi Biotec) using PBMCs from three donors as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ZFP36L2CKO and WT OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The cerebrum was then cut into 16 pieces and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec), according to the manufacturer’s protocol ...