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Citations for Miltenyi Biotec :
1001 - 1030 of 1030 citations for Cis Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%; 1 D 97% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Monocytes were cultured for six days at 37°C/5%CO2 in the presence of either M-CSF (50 ng/mL; Miltenyi Biotec) or GM-CSF (5 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions containing the IEC fraction were diluted to 108 cells/mL in MACs buffer (0.5% BSA 2mM EDTA) before being processed with the Miltenyi Dead Cell Removal Kit (Miltenyi Biotec, Auburn, CA) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... pooled honey bees (n=30) were dissociated with 15 mL of molecular grade water using gentleMACS™ M Tubes (Miltenyi Biotec, USA) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Cells were collected for TSDR demethylation analysis and stored at −80 as cell pellets or expanded with 500-1000 U/mL human rIL2 (Miltenyi Biotec/Chiron) +/− 100 nM rapamycin (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... The harvested lungs were homogenized in 4.5 ml of distilled water with the gentleMACS™ Tissue Dissociator (Miltenyi Biotec, Bergish Gladbach, Germany) and the homogenates were plated on Middlebrook 7H10–oleic acid ADC (7H10-OADC ...
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bioRxiv - Immunology 2019Quote: ... For genomic and transcriptomic analyses of activated LCs fresh migrated LCs from 3 donors were stimulated with TNF-α (25 ng/ml, Miltenyi Biotec, UK) for 2 ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 0.5 mL DPBS with 0.5 % BSA and labeled with 50 μL anti-CD14 microbeads (Miltenyi Biotec, catalog# 130-050-201). Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS™ C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS™ Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the SVF resuspended in 10 ml room temperature plain DMEM followed by additional filtering through a 30 μm mesh filter (Miltenyi Biotec # 130-041-407) and subsequent centrifugation at 700 g for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Alveolar-like MDMs were generated by differentiating CD4+ monocytes on tissue culture plates into macrophages in the presence of 50 ng/ml of human M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days ...
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bioRxiv - Developmental Biology 2021Quote: ... Differentiation was induced when cells reached 80-90% confluency using BPEL medium (Ng et al, 2008) supplemented with 20 ng/mL Activin-A (Miltenyi Biotec, #130-115-012), 20 ng/mL BMP4 (R&D systems ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in fresh media containing either 100 nM CCK or 100 nM saline vehicle control up to 72 hours with concurrent treatment with a mouse cytokine cocktail containing 50-ng/mL TNFα (Miltenyi Biotec, #139-101-687), 10-ng/mL IL-1β (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: We stimulated the spleen and lung cells from immunized and control animals to detect nucleocapsid protein-specific CD8+ T cell responses with an N protein-peptide pool at a concentration of 1.5 μg/mL/peptide (Miltenyi Biotec; 130-126-699, Germany) at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029 ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were differentiated into a macrophage-like phenotype (MDM, monocyte derived macrophages) using M-CSF (50 ng/mL, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Bioengineering 2024Quote: ... Canada) for 48 hours or T-cell TransAct (10 uL for each mL of culture, Cat # 130-111-160, Miltenyi Biotec, Bergisch Gladbach, Germany) for 72 hours respectively.
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bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Neuroscience 2022Quote: ... At the end of the incubation the single cell suspension was washed with 500 μL wash buffer (0.02% BSA in 1X PBS with 80 U/mL Protector RNase Inhibitor) and passed through a 20 μM filter (MACS, Miltenyi Biotec cat#130-101-812) to a fresh 2 mL Protein LoBind Eppendorf ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One ...
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bioRxiv - Bioengineering 2023Quote: ... Media supplements tested were FGF-2 at a concentration of 10 ng/ml (Human FGF-2 IS premium grade, MACS Miltenyi Biotec, Germany, 130-104-922), EGF at a concentration of 10 ng/ml (Recombinant Human EGF ...
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bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
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bioRxiv - Microbiology 2023Quote: MEFs were seeded at 5.104 cells per well in 24-well plates one day before stimulation and treated with 300 IU/mL IFNα (Miltenyi Biotec Cat#130-093-131) and incubated for up to 24 hours.
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bioRxiv - Genetics 2022Quote: ... 150 µl of 1M HEPES and 0.75 µl of 10 mg/ml DNAse I) and dissociated in gentleMACS Dissociator (Miltenyi Biotec Inc., cat. no. 130-093-235) using the Multi_E_01.01 program ...