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Citations for Miltenyi Biotec :
951 - 1000 of 1011 citations for Anti NET SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
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bioRxiv - Immunology 2021Quote: ... Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec, USA). To obtain high purity Treg ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2022Quote: ... Yeast cells were washed 5 times with PBSF (PBS + 0.1% bovine serum albumin (BSA)) and 108 cells were incubated with 400 μL PBSF and 100 μL μMACS™ anti-c-Myc MicroBeads (Miltenyi Biotec) for 45 min on a rotator at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Developmental Biology 2021Quote: ... Positive selection was subsequently applied with an antibody against biotin-conjugated integrin α7-biotin and anti-biotin MicroBeads (Miltenyi Biotec, 130-090-485), followed by MS columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... Purified stromal cells were then separated from epithelial cells using anti-CD326 (EpCAM) antibody-coated microbeads (Miltenyi Biotec Inc., San Diego, CA, USA). Next ...
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bioRxiv - Neuroscience 2019Quote: ... The lysate was cleared by centrifugation incubated with anti-GFP/myc antibody coated magnetic beads (MultiMACS GFP Isolation Kit #130-094-253 or Miltenyi Biotec GmbH, Germany). The co-immunoprecipitation was done following the manufacturer’s protocol (Mitenyibiotec ...
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bioRxiv - Neuroscience 2021Quote: ... containing cell viability dye – APCH7 (cat. no. 65-0865-14, eBioscience, 1:5000) and anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250), a specific marker of astrocytes ...
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bioRxiv - Microbiology 2020Quote: ... Human myeloid CD1c+ DC were isolated from blood and kidney by positive immuno-magnetic selection using anti-CD1c/BDCA-1 microbeads according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) or on a FACS ARIA (BD Biosciences ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 150 ul of α-c-HA magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-094-255) as described above ...
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bioRxiv - Neuroscience 2020Quote: ... cells dissociated as above were immunopurified with mouse monoclonal antibody to Thy-1 (BSJ-1: French and Jeffrey, 1986) using goat anti-mouse IgG conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) (Yamagata et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 30 µl of α-c-Myc magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-091-284) and incubated for 2 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 0.5 mL DPBS with 0.5 % BSA and labeled with 50 μL anti-CD14 microbeads (Miltenyi Biotec, catalog# 130-050-201). Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2022Quote: ... Non-plasma cells were labeled with a biotin-conjugated antibody cocktail combined with anti-biotin microbeads and separated using a magnetic rack according to the manufacturer’s protocol (Miltenyi Biotec, 130-092-530). Purified plasma B cells were eluted and sequentially incubated with CD138 microbeads for an additional 15 minutes at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Anti-Biotin MicroBeads (Miltenyi Biotec, # 130-115-389) and incubated for 10 minutes at 4 °C ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... PBMC were thawed and monocytes and peripheral blood lymphocytes (PBL) were separated using human anti-CD14 Ab-coated microbeads to positively select monocytes (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were harvested and mitochondria were purified using anti-TOMM22 labelled magnetic micro-beads according to the manufacturer’s instructions (Miltenyi Biotec 130-094-532). 50 µg of purified mitochondria were subjected to Western blot analysis and 10% of total lysate was loaded as positive control.
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bioRxiv - Cell Biology 2022Quote: ... for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2, Miltenyi Biotec, 130-117-701) or corresponding IgG1-APC isotype (clone IS5-21F5 ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant containing the resuspended proteins was used for immunoprecipitation assay using anti-GFP microbeads provided in the μMACS Epitope Tag Protein Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Approximately ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec, 130-049-601) and an anti-O4 microbeads antibody were used to isolate microglia and oligodendrocytes ...
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bioRxiv - Bioengineering 2024Quote: ... were obtained after transduction of Jurkat T cells with a serial dilution of viral supernatants and transduction efficiency evaluated after 3-4 days by monitoring cell surface dNGFR expression using an anti-CD271-APC antibody (Miltenyi Biotec., Paris, France) or HLA-A*02 Dextramer-APC (Immudex ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ HSPC were enriched from cord blood mononuclear cells (MNC) by magnetic selection using anti-CD34 conjugated magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec, 130-100-453). To obtain a sufficient cell number for ImageStream and Western blot analysis ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were adjusted to 5 mg/mL protein concentration and incubated for 1 h at 4 °C with 100 μL of anti-GFP magnetic beads (Miltenyi Biotec, #130-091-125). Bound proteins were analyzed by immunoblotting with anti-GFP (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Immunology 2019Quote: ... That included labeling first with CD154-biotin and magnetically anti-biotin microbeads in a second step followed by enrichment using MS MACS columns (Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2019Quote: ... 2 × 107 PBMC/ml/well were stimulated for 6h with excretory-secretory antigen mixtures of female Ascaris suum worms (ESF, 40 µg/ml) in the presence of anti-CD40 (clone HB14, 1 µg/ml functional grade pur, Miltenyi Biotec, Bergisch Gladbach, Germany) and anti-CD28 (clone CD28.2 ...
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bioRxiv - Molecular Biology 2019Quote: For isolating the SSEA1 positive cells from reprogramming intermediates at day6 and day9 we used magnetic microbeads conjugated to anti-SSEA1 antibody (MACS Miltenyi Biotec #130-094-530) as per manufacture instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were cultured for one week and then the same procedure was followed with anti-Prominin-1 MicroBeads (Miltenyi Biotec, Cat# 130-092-564) for the negative selection of radial glia ...
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bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: B cells were purified from splenocytes by positive selection using magnetic beads-conjugated monoclonal anti-mouse B220 antibody (clone: RA3-6B2, Miltenyi Biotec, Bergisch Gladbach, Germany). Isolated B cells (5 × 105/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM EDTA in PBS) for 15 minutes and after two washes the samples were incubated with anti-biotin-APCVio770 (Miltenyi Biotec #130-113-851), diluted 1:50 in FACS buffer for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2021Quote: αβT cells transduced with γδTCR-Cl5 either alone or together with NKG2D wild type or the different NKG2D chimeras were incubated with a biotin-labeled anti-αβTCR antibody (clone BW242/412; Miltenyi Biotec, Bergisch Gladbach, Germany) and subsequently incubated with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Cancer Biology 2022Quote: ... and efficiently depleted of non-target cells by addition of magnetic microbeads conjugated to mouse anti-biotin monoclonal IgG1 antibodies and passage through LS ferromagnetic columns (Miltenyi Biotec, 130-042-401). The flow through ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with anti-Biotin mAb microbeads to deplete αβT-cells by magnetic separation using AUTOMACS under depleteS protocol (Miltenyi Biotec, Biergisch Gladbach, Germany). DOT cells were generated from αβ-depleted PBMCs cultured in either 96-well plates or G-REX platform (Wilson Wolf Manufacturing) ...
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bioRxiv - Plant Biology 2022Quote: ... isolated from transfected protoplasts were separated by SDS-PAGE and subjected to immunoblotting using an anti-GFP-HRP antibody (130-091-833, Miltenyi Biotec, dilution 1:5000).
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained for 30 min at 4 °C in the dark in PBS using the following antibodies and conjugates: qNSCs and aNSCs were discriminated by a combination of PE-conjugated anti-mGLAST (1:50, clone ACSA-1, Miltenyi Biotec, Bergisch Gladbach, Germany), APC-conjugated anti-mCD133 (1:75 ...
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bioRxiv - Neuroscience 2023Quote: ... cortical organoids were then enzymatically dissociated with AccuMax and purified by MACS using anti-PSA-NCAM MicroBeads (Miltenyi Biotec, cat. no. 130-097-859) and plated onto poly-D-ornithine and laminin-coated coverslips or laminin-coated culture plates and further maintained in Brainphys medium ...
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bioRxiv - Microbiology 2023Quote: ... CD14+ cells were isolated from the PBMCs using human CD14+ micro beads conjugated with anti-human CD14 mAbs (130-118-906, Miltenyi Biotec, Bergisch Gladbach, Germany) LC columns (130-042-401 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...